Journal List > J Nutr Health > v.52(1) > 1117285

J Nutr Health. 2019 Feb;52(1):17-25. Korean.
Published online Feb 28, 2019.  https://doi.org/10.4163/jnh.2019.52.1.17
© 2019 The Korean Nutrition Society
Effects of Lonicera caerulea extract on adipocyte differentiation and adipogenesis in 3T3-L1 cells and mouse adipose-derived stem cells (MADSCs)
Miey Park,1 Changho Lee,2 and Hae-Jeung Lee1
1Department of Food and Nutrition, Gachon University, Seongnam, Gyeonggi 13120, Korea.
2Research Group of Functional Food Materials, Korea Food Research Institute, Wanju, Jeonbuk 55365, Korea.

To whom correspondence should be addressed. tel: +82-31-750-5968, Email: skysea@gachon.ac.kr ,Email: skysea1010@gmail.com
Received Jan 09, 2019; Revised Jan 18, 2019; Accepted Feb 09, 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

Obesity is a major health problem of global significance because it is clearly associated with an increased risk of health problems, such as nonalcoholic fatty liver disease (NAFLD), diabetes, cardiovascular diseases, and cancer. Lonicera caerulea (LC) originates from high mountains or wet areas and has been used as a traditional medicine in northern Russia, China, and Japan. LC contains a range of bioactive constituents, such as vitamins, minerals, and polyphenols. This study examined the anti-obesity effects of LC during differentiation in preadipocytes.

Methods

The cell viability assay was performed after the differentiation of 3T3-L1 cells for 7 days. Oil Red O staining was used to visualize the changes in lipid droplets in 3T3-L1 cells and mouse adipose-derived stem cells (MADSCs). The mRNA expression of obesity-related genes was determined by quantitative real-time PCR.

Results

According to the results of Oil Red O staining, the lipid levels and size of lipid droplets in the adipocytes were reduced and the LC extract (LCE, 0.25–1 mg/mL) markedly inhibited adipogenesis in a dose-dependent manner. The treatment of LCE also decreased the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein-α (C/EBPα), and sterol regulatory element binding protein 1 (SREBP1) in 3T3-L1 cells. Western blot analysis showed that the PPARγ, C/EBPα, and SREBP1 protein levels in both 3T3-L1 and MADSC were reduced in a dose-dependent manner.

Conclusion

These results suggest that LCE can inhibit adipogenic differentiation through the regulation of adipogenesis-related markers.

Keywords: Lonicera caerulea; preadipocyte; anti-obesity; lipid accumulation

Figures


Fig. 1
Effect of Lonicera caerulea extract (LCE) on 3T3-L1 preadipocytes proliferation. 3T3-L1 preadipocyte cells were treated with the indicated concentrations of LCE for 24, 48, and 72hr and cell viability was estimated by a CCK-8 assay. All experiments were repeated at least three times and data represent means ± SD. ** p < 0.01, *** p < 0.001 vs. Con.
Click for larger image


Fig. 2
Lipid accumulation was determined by Oil Red O staining. LCE treated 3T3-L1 adipocytes (A) and lipid levels in 3T3-L1 adipocytes (B). Original magnification 200X. Data represent means ± SD. ** p < 0.01, *** p < 0.001 vs. Con.
Click for larger image


Fig. 3
Lipid accumulation was determined by Oil Red O staining. LCE treated mouse adipose-derived stem cell (MADSC) adipocytes (A) and lipid levels in MADSC adipocytes (B). Original magnification 200X. Data represent means ± SD. *** p < 0.001 vs. Con.
Click for larger image


Fig. 4
Effects of LCE on the expression of genes associated with adipogenesis in 3T3-L1 cells. The expression of Pparγ (A), C/ebpα (B), and Srebp1 (C) were quantified by real-time PCR and normalized by β-actin as an internal control. (D) Western blot analysis of PPARγ, C/EBPα, and SREBP1 protein. Data represent means ± SD. * p < 0.05, ** p < 0.01 vs. Con.
Click for larger image


Fig. 5
Effects of LCE on the expression of protein associated with adipogenesis in MADSCs. The protein expression of PPARγ (A), C/EBPα (B), and SREBP1 (C) were normalized by β-actin as an internal control. (D) PPARγ, C/EBPα, and SREBP1 protein levels by Immunoblot analysis. Data represent means ± SD. * p < 0.05, ** p < 0.01 vs. Con.
Click for larger image

Tables


Table 1
Sequences of primers for quantitative real-time PCR in this research
Click for larger image

Notes

This work was supported by a grant ‘Cooperative Research Program for Agriculture Science and Technology Development funded by Rural Development Administration (Project No. PJ01227802) and Korea Food Research Institute (grant E0150302-04), Republic of Korea’.

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