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Journal List > Korean J Lab Med > v.30(6) > 1011710

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Korean J Lab Med. 2010 Dec;30(6):668-674. Korean.
Published online December 02, 2010.  https://doi.org/10.3343/kjlm.2010.30.6.668
Copyright © 2010 The Korean Society for Laboratory Medicine
Discrepancies between Human Leukocyte Antigen Registry Typing and Confirmatory Typing Results of Unrelated Hematopoietic Stem Cell Donors
Dae-Hyun Ko, M.D., Hye Yoon Chung, M.D., Young Mi Lim, M.T., Bok Youn Han, M.T., Eun Young Song, M.D. and Myoung Hee Park, M.D.
Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.

Corresponding author: Myoung Hee Park, M.D. Department of Laboratory Medicine, Seoul National University College of Medicine, 101 Daehang-no, Jongno-gu, Seoul 110-744, Korea. Tel: +82-2-2072-3388, Fax: +82-2-3672-3337, Email: parkmhee@snu.ac.kr
Received May 31, 2010; Revised October 01, 2010; Accepted October 14, 2010.

Abstract

Background

In unrelated hematopoietic stem cell transplantation, the accuracy of HLA registry typing (RT) of donors is important for timely search and coordination of HLA-matched donors. We analyzed discrepancies between HLA RT and confirmatory typing (CT) results of stem cell donors in Korean and foreign registries.

Methods

We analyzed the HLA typing results of 834 donors for whom CT was performed at Seoul National University Hospital between April 1997 and March 2010. For CT, DNA typing was used in majority of the cases and HLA-A and HLA-B serological typing was used in some early cases. The discrepancies between the typing results were analyzed at the serological/generic level.

Results

The overall discrepancy rate (RT error rate) was 3.2%, and the rate was similar in the Korean and foreign registries. The discrepancy rates in the Korean and foreign registries were more than 10% in the 1997-2001 searches, but decreased to less than 3% in the 2002-2010 searches. Analysis of 19 cases of RT errors in the Korean registry revealed 3 cases of sample switchover errors and 16 cases of typing errors in one of the HLA-A, HLA-B, or HLA-DR loci. The RT error rate in Japan Marrow Donor Program was lower than those in other foreign registries.

Conclusions

The error rate of HLA RT results of unrelated stem cell donors in the Korean registry was similar to those in the foreign registries, and has decreased in the recent searches following the change in the typing method from serological to DNA typing.

Keywords: HLA; Hematopoietic stem cell; Transplantation; Donor registry; Error rate

Tables


 Table 1
Type of errors in HLA registry typing of unrelated stem cell donors
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Table 2
Error rate in HLA registry typing: Korean registry vs. foreign registries
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Table 3
Error rate in HLA registry typing in different foreign registries
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Table 4
HLA registry typing errors in donors searched from the Korean registry (N=19)
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Table 5
HLA registry typing errors in donors searched from foreign registries (N=8)
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http://www.efiweb.eu/index.php?id=102.
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