Journal List > J Nutr Health > v.52(5) > 1136449

J Nutr Health. 2019 Oct;52(5):413-421. Korean.
Published online Oct 30, 2019.  https://doi.org/10.4163/jnh.2019.52.5.413
© 2019 The Korean Nutrition Society
Changes in the constituents and UV-photoprotective activity of Astragalus membranaceus caused by roasting
Jeong-Yong Park,1,2 Ji Yeon Lee,1,2 Hyung Don Kim,1 Gwi Yeong Jang,1 and Kyung Hye Seo1
1Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumsung, Chungbuk 27709, Korea.
2Department of Food Science and Biotechnology, Chungbuk National University, Chungbuk 28644, Korea.

To whom correspondence should be addressed. tel: +82-43-871-5785, Email: seokh@korea.kr
Received Jun 11, 2019; Revised Aug 05, 2019; Accepted Sep 03, 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

Astragalus membranaceus (AM) is an important traditional medicinal herb. Pharmacological research has indicated that AM has various physiological activities such as antioxidant, anti-inflammatory, immunoregulatory, anticancer, hypolipidemic, antihyperglycemic, and hepatoprotective activities. The bioactive substances responsible for the physiological activities in AM, including many antioxidant substances, change during the roasting process. This study investigated and compared the changes in the antioxidant constituents of AM caused by roasting.

Methods

DPPH (1,1-diphenyl-2-picryl hydrazyl) and ABTS+ (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt) radical scavenging activities and their total phenolic content (TPC) were measured. High-performance liquid chromatography (HPLC) analysis was performed to confirm any changes in the isoflavonoids of roasted AM (R-AM),. The cell viability of UVB-induced HDF (Human dermal fibroblast) cells treated with AM and R-AM extracts was investigated. The comet assay was used to examine the inhibitory effects of R-AM extracts on DNA damage caused by oxidative stress.

Results

The DPPH and ABTS+ radical scavenging activities were 564.6±20.9 and 108.2±3.1 (IC50 value) respectively, from the 2R-AM. The total phenol content was 47.80±1.40 mg GAE/g from the 1R-AM. The values of calycosin and formononetin, which are the known isoflavonoid constituents of AM, were 778.58±2.72 and 726.80±3.45 µg/g respectively, from the 2R-AM. Treatment of the HDF cells with R-AM (50 ~ 200 µg/mL) did not affect the cell viability. Furthermore, the R-AM extracts effectively protected against UVB-induced DNA damage.

Conclusion

The findings of this study indicate that R-AM increases its isoflavonoid constituents and protects against UVB-induced DNA damage in HDF cells.

Keywords: Astragalus membranaceus; UV-photoprotective activity; antioxidant; DNA damage

Figures


Fig. 1
Chromatogram of AMs using HPLC analysis. (A) Scheme for AM samples. NR-AM, none roasted-Astragalus membranaceus; 1R-AM, 1st roasted-Astragalus membranaceus; 2R-AM, 2nd roasted-Astragalus membranaceus; 3R-AM, 3rd roasted-Astragalus membranaceus; B–F, Chromatogram of AMs using HPLC analysis; 1, daidzein-7-O-β-D-glucoside; 2, calycosin-7-O-β-D-glucoside; 3, formononetin-7-O-β-D-glucoside; 4, daidzein; 5, calycosin; 6, formononetin.
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Fig. 2
Cell viability of AMs on HDF cells. HDF cells were treated R-AM samples of 50, 100 and 200 µg/mL for 24 h. Cell viability was examined using MTS assay. NR-AM, none roasted-Astragalus membranaceus; 1R-AM, 1st roasted-Astragalus membranaceus; 2R-AM, 2nd roasted-Astragalus membranaceus; 3R-AM, 3rd roasted-Astragalus membranaceus.
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Fig. 3
Inhibitory effect of AMs during repair of UVB-induced DNA damage on HDF cells. HDF cells were cultured in 96-well plates and exposed to the indicated fluorescent of UVB. Single cells were embedded in agarose immediately after UV irradiation, electrophoresed and photographed using a fluorescent microscope to determine the “Tail moment” of each Tail length * % DNA in tail (representing the extent of DNA damage). NR-AM, none roasted-Astragalus membranaceus; 1R-AM, 1st roasted-Astragalus membranaceus; 2R-AM, 2nd roasted-Astragalus membranaceus; 3R-AM, 3rd roasted-Astragalus membranaceus; (A) Representative images of wild and UVB-induced HDF cells. (B) Tail DNA intensity was quantified by each 3 arrow points in the Fig. 1A. Results are significantly different among groups by Duncan's multiple range test (p < 0.05).
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Tables


Table 1
Antioxidant activity of AMs
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Table 2
Total phenolic contetns of AMs
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Table 3
The major isoflavonoid contents of AMs
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Table 4
The correlation coefficients between antioxidant activities and AM1) contents
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Notes

This study was performed with the support of the Cooperative Research Program for Agriculture Science and Technology Development (project no. PJ01273003), the Rural Development Administration, Republic of Korea.

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