Journal List > J Nutr Health > v.52(2) > 1122026

J Nutr Health. 2019 Apr;52(2):149-156. Korean.
Published online Apr 30, 2019.
© 2019 The Korean Nutrition Society
Dietary effect of Lactobacillus plantarum CJLP55 isolated from kimchi on skin pH and its related biomarker levels in adult subjects
Sangshin Han,1 Jihye Shin,1 Sunhee Lim,1 Hee Yoon Ahn,2 Bongjoon Kim,2 and Yunhi Cho1
1Department of Medical Nutrition, Graduate School of East-West Medical Science, Kyung Hee University, Yongin, Gyeonggi 17104, Korea.
2CJ Foods R&D Center, CJ CheilJedang Corporation, Suwon, Gyeongggi 16495, Korea.

To whom correspondence should be addressed. tel: +82-31-201-3817, Email:
Received Dec 10, 2018; Revised Feb 14, 2019; Accepted Feb 14, 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.



The skin pH is maintained by epidermal lactate, free fatty acids (FFAs), and free amino acids (FAAs). As a significant determinant of skin health, the skin pH is increased (less acidic) under abnormal and aged skin conditions. In a search for dietary alternatives that would promote an acidic skin pH, this study investigated the dietary effects of Lactobacillus plantarum CJLP55 isolated from Korean kimchi on the skin pH, and epidermal levels of lactate, FFAs, and FAAs in adult subjects.


Seventy eight subjects (mean age 24.9 ± 0.5 years, range 19 ~ 37 years) were assigned randomly to ingest CJLP55, Lactobacillus strain from kimchi, (n = 39, CJLP group) or placebo supplements (n = 39, placebo group) for 12 weeks in a double-blind, placebo-controlled trial. Skin pH and epidermal levels of lactate, FFAs and FFAs were assessed at 0, 6 and 12 weeks.


Although significant decreases in skin pH were observed in both the CJLP and placebo groups at 6 weeks, the skin pH was decreased significantly only in the CJLP group at 12 weeks. In parallel, the epidermal level of lactate in the CJLP group was also increased by 25.6% at 12 weeks. On the other hand, the epidermal level of FAAs were not altered in the CJLP and placebo groups, but the epidermal level of total FFAs, including palmitic acid and stearic acid, was lower in the CJLP group than in the placebo group over 12 weeks. The changes in the other FFAs, such as palmitoleic acid and oleic acid, were similar in the CJLP and placebo groups over 12 weeks.


Overall, a dietary supplement of CJLP55 promotes acidic skin pH with a selective increase in epidermal lactate in adult subjects.

Keywords: skin pH; Lactobacillus plantarum; lactate; free amino acid; free fatty acid


Fig. 1
Altered skin pH in groups. Skin surface pH was measured by skin pH meter. All values are means ± SEM (n = 39 / group). Differences from 0 week within CJLP or Placebo groups were evaluated by Student's paired t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Differences between CJLP and Placebo groups were evaluated by Student's unpaired t-test (#p < 0.05).
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Fig. 2
Altered free fatty acid (FFA) levels in the skin surface of groups. Individual FFA was fractionated by high-performance thin-layer chromatography, eluted, and further analyzed by gas chromatography after acid methanolysis. All values are mean ± SEM (n = 39 / group). Differences from 0 week within CJLP or Placebo groups were evaluated by paired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001). Differences between CJLP and Placebo groups were evaluated by unpaired Student's t-test (#p < 0.05).
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Table 1
Altered lactate levels in the skin surface of groups
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Table 2
Altered free amino levels in the skin surface of groups
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This study was supported by a grant from CJ Foods R&D Center, CJ CheilJedang Corporation in Suwon-si, Gyeongggi-do, Republic of Korea (KHU grant No. 20150943).

1. Fluhr JW, Elias PM. Stratum corneum pH: formation and function of the ‘acid mantle’. Exogenous Dermatology 2002;1(4):163–175.
2. Schmid-Wendtner MH, Korting HC. The pH of the skin surface and its impact on the barrier function. Skin Pharmacol Physiol 2006;19(6):296–302.
3. Thurmon FM, Ottenstein B. Studies on the chemistry of human perspiration with especial reference to its lactic acid content. J Invest Dermatol 1952;18(4):333–339.
4. Rawlings AV, Harding CR. Moisturization and skin barrier function. Dermatol Ther 2004;17 Suppl 1:43–48.
5. Garidel P, Fölting B, Schaller I, Kerth A. The microstructure of the stratum corneum lipid barrier: mid-infrared spectroscopic studies of hydrated ceramide:palmitic acid:cholesterol model systems. Biophys Chem 2010;150(1-3):144–156.
6. Goffin P, Lorquet F, Kleerebezem M, Hols P. Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase. J Bacteriol 2004;186(19):6661–6666.
7. Fluhr JW, Kao J, Jain M, Ahn SK, Feingold KR, Elias PM. Generation of free fatty acids from phospholipids regulates stratum corneum acidification and integrity. J Invest Dermatol 2001;117(1):44–51.
8. Suzuki N, Ishizaki J, Yokota Y, Higashino K, Ono T, Ikeda M, et al. Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A2s. J Biol Chem 2000;275(8):5785–5793.
9. Matsui T, Miyamoto K, Kubo A, Kawasaki H, Ebihara T, Hata K, et al. SASPase regulates stratum corneum hydration through profilaggrin-to-filaggrin processing. EMBO Mol Med 2011;3(6):320–333.
10. Sandilands A, Sutherland C, Irvine AD, McLean WH. Filaggrin in the frontline: role in skin barrier function and disease. J Cell Sci 2009;122(Pt 9):1285–1294.
11. Eberlein-König B, Schäfer T, Huss-Marp J, Darsow U, Möhrenschlager M, Herbert O, et al. Skin surface pH, stratum corneum hydration, trans-epidermal water loss and skin roughness related to atopic eczema and skin dryness in a population of primary school children. Acta Derm Venereol 2000;80(3):188–191.
12. Choi EH, Man MQ, Xu P, Xin S, Liu Z, Crumrine DA, et al. Stratum corneum acidification is impaired in moderately aged human and murine skin. J Invest Dermatol 2007;127(12):2847–2856.
13. Nakagawa N, Sakai S, Matsumoto M, Yamada K, Nagano M, Yuki T, et al. Relationship between NMF (lactate and potassium) content and the physical properties of the stratum corneum in healthy subjects. J Invest Dermatol 2004;122(3):755–763.
14. Béke G, Dajnoki Z, Kapitány A, Gáspár K, Medgyesi B, Póliska S, et al. Immunotopographical differences of human skin. Front Immunol 2018;9:424.
15. Bourrie BC, Willing BP, Cotter PD. The microbiota and health promoting characteristics of the fermented beverage kefir. Front Microbiol 2016;7:647.
16. Guéniche A, Philippe D, Bastien P, Blum S, Buyukpamukcu E, Castiel-Higounenc I. Probiotics for photoprotection. Dermatoendocrinol 2009;1(5):275–279.
17. Valdéz JC, Peral MC, Rachid M, Santana M, Perdigón G. Interference of Lactobacillus plantarum with Pseudomonas aeruginosa in vitro and in infected burns: the potential use of probiotics in wound treatment. Clin Microbiol Infect 2005;11(6):472–479.
18. Lee DE, Huh CS, Ra J, Choi ID, Jeong JW, Kim SH, et al. Clinical evidence of effects of Lactobacillus plantarum HY7714 on skin aging: a randomized, double blind, placebocontrolled study. J Microbiol Biotechnol 2015;25(12):2160–2168.
19. Kim H, Kim HR, Jeong BJ, Lee SS, Kim TR, Jeong JH, et al. Effects of oral intake of kimchi-derived Lactobacillus plantarum K8 lysates on skin moisturizing. J Microbiol Biotechnol 2015;25(1):74–80.
20. Giri SS, Sen SS, Saha S, Sukumaran V, Park SC. Use of a potential probiotic, Lactobacillus plantarum L7, for the preparation of a rice-based fermented beverage. Front Microbiol 2018;9:473.
21. Lew LC, Liong MT. Bioactives from probiotics for dermal health: functions and benefits. J Appl Microbiol 2013;114(5):1241–1253.
22. Šeme H, Gjuračić K, Kos B, Fujs Š, Štempelj M, Petković H, et al. Acid resistance and response to pH-induced stress in two Lactobacillus plantarum strains with probiotic potential. Benef Microbes 2015;6(3):369–379.
23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193(1):265–275.
24. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem 1957;226(1):497–509.
25. Di Giorgio J. Determination of serum lactic dehydrogenase isoenzymes by use of the “Diagnostest” cellulose acetate electrophoresis system. Clin Chem 1971;17(4):326–331.
26. Imahashi D. Quantitation of LDH isoenzymes by the fluorimetric and colorimetric methods. Can J Med Technol 1968;30(6):235–248.
27. Uchida Y, Hara M, Nishio H, Sidransky E, Inoue S, Otsuka F, et al. Epidermal sphingomyelins are precursors for selected stratum corneum ceramides. J Lipid Res 2000;41(12):2071–2082.
28. Tang W, Ziboh VA. Reversal of epidermal hyperproliferation in essential fatty acid deficient guinea pigs is accompanied by rapid generation of inositol triphosphate. Arch Dermatol Res 1988;280(5):286–292.
29. Wesley NO, Maibach HI. Racial (ethnic) differences in skin properties: the objective data. Am J Clin Dermatol 2003;4(12):843–860.
30. Jacobi U, Gautier J, Sterry W, Lademann J. Gender-related differences in the physiology of the stratum corneum. Dermatology 2005;211(4):312–317.
31. Visscher MO, Chatterjee R, Munson KA, Pickens WL, Hoath SB. Changes in diapered and nondiapered infant skin over the first month of life. Pediatr Dermatol 2000;17(1):45–51.
32. Hoeger PH, Enzmann CC. Skin physiology of the neonate and young infant: a prospective study of functional skin parameters during early infancy. Pediatr Dermatol 2002;19(3):256–262.
33. Zlotogorski A. Distribution of skin surface pH on the forehead and cheek of adults. Arch Dermatol Res 1987;279(6):398–401.
34. Lee B, Kim J, Hwang J, Cho Y. Dietary effect of green tea extract on epidermal levels of skin pH related factors, lactate dehydrogenase protein expression and activity in UV-irradiated hairless mice. J Nutr Health 2016;49(2):63–71.
35. Sugawara T, Kikuchi K, Tagami H, Aiba S, Sakai S. Decreased lactate and potassium levels in natural moisturizing factor from the stratum corneum of mild atopic dermatitis patients are involved with the reduced hydration state. J Dermatol Sci 2012;66(2):154–159.
36. Cabanillas B, Novak N. Atopic dermatitis and filaggrin. Curr Opin Immunol 2016;42:1–8.
37. Akaza N, Akamatsu H, Numata S, Matsusue M, Mashima Y, Miyawaki M, et al. Fatty acid compositions of triglycerides and free fatty acids in sebum depend on amount of triglycerides, and do not differ in presence or absence of acne vulgaris. J Dermatol 2014;41(12):1069–1076.
38. Lewis C Jr, Schmitt M, Hershey FB. Heterogeneity of lactic dehydrogenase of human skin. J Invest Dermatol 1967;48(3):221–225.
39. Ronquist G, Andersson A, Bendsoe N, Falck B. Human epidermal energy metabolism is functionally anaerobic. Exp Dermatol 2003;12(5):572–579.
40. Boussouar F, Grataroli R, Ji J, Benahmed M. Tumor necrosis factor-alpha stimulates lactate dehydrogenase A expression in porcine cultured sertoli cells: mechanisms of action. Endocrinology 1999;140(7):3054–3062.
41. Huang D, Hubbard CJ, Jungmann RA. Lactate dehydrogenase A subunit messenger RNA stability is synergistically regulated via the protein kinase A and C signal transduction pathways. Mol Endocrinol 1995;9(8):994–1004.
42. Haza AI, Zabala A, Morales P. Protective effect and cytokine production of a Lactobacillus plantarum strain isolated from ewes' milk cheese. Int Dairy 2004;14(1):29–38.