Journal List > J Nutr Health > v.52(1) > 1117294

J Nutr Health. 2019 Feb;52(1):6-16. Korean.
Published online Feb 28, 2019.  https://doi.org/10.4163/jnh.2019.52.1.6
© 2019 The Korean Nutrition Society
Effect of Sulgidduk containing pine needle juice on lipid metabolism in high fat-cholesterol diet induced dyslipidemic rats
Yunjung Lee, Jae-Hee Park and Eunju Park
Department of Food and Nutrition, Kyungnam University, Changwon, Gyeongnam 51767, Korea.

To whom correspondence should be addressed. tel: +82-55-249-2218, Email: pej@kyungnam.ac.kr
Received Jan 03, 2019; Revised Jan 13, 2019; Accepted Jan 30, 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

Dyslipidemia is a major risk factor for cardiovascular disease. Pine needles (Pinus densiflora seib et Zucc) are a traditional medicine used to treat dyslipidemia in clinical settings. This study examined the potential effects of sulgidduk, a Korean traditional rice cake containing pine needle juice to protect against dyslipidemia induced by a high-fat/sugidduk diet in a rat model.

Methods

Twenty one male Sprague-Dawley rats were divided randomly into three groups: normal control (NC), Sulgidduk diet (SD), Sulgidduk diet containing pine needle juice (PSD). The blood lipid levels, production of lipid peroxide in the plasma and liver, total cholesterol and triglyceride in the liver and feces, antioxidant enzyme activities in plasma and erythrocytes were measured to assess the effects of PSD on dyslipidemia.

Results

A high-fat/Sulgidduk diet induced dyslipidemia, which was characterized by significantly altered lipid profiles in the plasma and liver. The food intake was similar in the three groups, but weight gain and food efficiency ratio (FER) were reduced significantly in the PSD group compared to those in the SD group. The level of total cholesterol, LDL-cholesterol and TBARS in the plasma showed tendencies to decrease in the PSD group compared to those in the SD group. The levels of high-fat/Sulgidduk diet-induced sterol regulatory element-binding protein 2 (SREBP2) gene expression were reduced significantly in the PSD group. The supplementation of PSD reduced the hepatic triglyceride and total cholesterol levels significantly, and enhanced the fecal excretion of triglyceride and hepatic antioxidant enzyme activities compared to the SD group.

Conclusion

These results suggest that the addition of 0.4% pine needle juice to Sulgidduk may be an alternative snack to control dyslipidemia.

Keywords: pine needle juice; sulgidduk; dyslipidemia; SREBP2; hepatic lipid profile

Figures


Fig. 1
Effect of Sulgidduk containing pine needle juice on liver weight (A), liver triglyceride (B), liver total cholesterol (C), and fecal triglyceride (D) in rats fed a high fat diet. Each bar represents the mean ± SE. Bars with different superscript letters are significantly different at p < 0.05 by Duncan's multiple range test. ND, normal diet; SD, Sulgidduk diet; PSD, Sulgidduk diet containing pine needle juice
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Fig. 2
Effect of Sulgidduk containing pine needle juice on hepatic mRNA levels of SREBP2 genes in rats. Each bar represents the mean ± SE. Bars with different letters are significantly different at p < 0.05 by Duncan's multiple range test. ND, normal diet; SD, Sulgidduk diet; PSD, Sulgidduk diet containing pine needle juice
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Tables


Table 1
Composition of the experimental diets (g/kg diet)
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Table 2
Effects of Sulgidduk containing pine needle juice on weight gain and food intake in rats fed a high-fat diet
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Table 3
Effects of Sulgidduk containing pine needle juice on blood biochemical parameters in rats fed a high-fat diet
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Table 4
Effects of Sulgidduk containing pine needle juice on plasma and hepatic lipid peroxide level in rats fed a high-fat diet
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Table 5
Effects of Sulgidduk containing pine needle juice on antioxidant status in plasma and liver in rats fed a high-fat diet
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Notes

This work was supported by grants from Ministry of Agriculture, Food and Rural Affairs.

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