Journal List > J Nutr Health > v.52(1) > 1117286

J Nutr Health. 2019 Feb;52(1):26-35. Korean.
Published online Feb 28, 2019.  https://doi.org/10.4163/jnh.2019.52.1.26
© 2019 The Korean Nutrition Society
Antioxidant activity of ethanol extract of Lycium barbarum's leaf with removal of chlorophyll
Ji Eun Kim,1 Su Mi Bae,1 You Ree Nam,1 Eun Young Bae,1,2 and Sun Yung Ly1,2
1Department of Food and Nutrition, Chungam National University, Daejeon 34134, Korea.
2Convergence Research Center for Natural Products, Chungnam National University, Daejeon 34134, Korea.

To whom correspondence should be addressed. tel: +82-42-821-6838, Email: sunly@cnu.ac.kr
Received Nov 21, 2018; Revised Dec 17, 2018; Accepted Dec 28, 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

The aim of this study was to estimate the antioxidant activities of 50%, 70%, and 100% ethanol extracts of Lycium barbarum leaf and chlorophyll removal extract.

Methods

The antioxidant activities were estimated by measuring total polyphenol content and by assays of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfate) (ABTS) radical scavenging activities and ferric reducing antioxidant power (FRAP). In addition, reactive oxygen species (ROS) production, DNA fragmentation, and antioxidant enzyme (superoxide dismutase and catalase) activities of the extracts were measured in hydrogen peroxide (H2O2)-stressed HepG2 cells.

Results

The total polyphenol content, DPPH and ABTS radical scavenging activities, and FRAP value of the extracts increased in an ethanol concentration-dependent manner. The antioxidant activities of the chlorophyll-removal extracts were much higher than those of the chlorophyll-containing extracts. Cytotoxicity was not observed in HepG2 cells with extracts up to 1,000 µg/mL. All extracts inhibited ROS production in a concentration-dependent manner from 31.3 µg/mL and inhibited DNA damage at 250 µg/mL. The SOD and catalase activities of cell lines treated with the extracts and H2O2 were similar to those of normal cells, indicating a strong protective effect.

Conclusion

Lycium barbarum leaf extracts had high antioxidant activities and protected H2O2-stressed HepG2 cells. Since the chlorophyll-removal extract exhibited higher antioxidant activities than the chlorophyll-containing ones and the cytoprotective effect was similar, chlorophyll removal extract of Lycium barbarum leaf could be developed as ingredients of functional food and cosmetics.

Keywords: antioxidant; Lycium barbarum; HepG2 cell; chlorophyll removal

Figures


Fig. 1
Effect of the Lycium barbarum leave's ethanol extracts (LL50, LL70, LL100, LL100 Ch-) on HepG2 cell viability. LL 50, 70 and 100: Lycium barbarum's leaf extracted with 50, 70 and 100% ethanol, respectively; Group LL100 Ch-: chlorophyll removal ethanol extract. Values are mean ± standard deviation of three replicate determinations (n = 3). Different letters above the bars indicate statistically significant differences (p < 0.05).
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Fig. 2
Effects of (A) 50% ethanol extracs, (B) 70% ethanol extract, (C) 100% ethanol extract of Lycium barbarum's leaf and (D) chlorophyll removal extract with 100% ethanol from Lycium barbarum's leaf on the production of intracellular ROS level in H2O2-treated HepG2 cells. Values are mean ± SD of three replicate determinations (n = 3). Different superscripts (a–c) in a column indicate significant differences at p < 0.05 by Duncan's multiple range test (*: p < 0.05, compared to treated only H2O2).
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Tables


Table 1
Chlorophyll a and total polyphenol contents of Lycium barbarum's leaf extracts
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Table 2
Antioxidant capacities of Lycium barbarum's leaf extracts
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Table 3
Levels of DNA damage expressed as tail DNA, tail length, and tail moment in HepG2 cells with or without Lycium barbarum's leaf extracts
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Table 4
Effects of Lycium barbarum's leaf extracts on the antioxidant enzyme activities in HepG2 cells
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Notes

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2017R1D1A3B03028628).

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