Journal List > J Nutr Health > v.51(4) > 1100553

J Nutr Health. 2018 Aug;51(4):323-329. Korean.
Published online August 31, 2018.  https://doi.org/10.4163/jnh.2018.51.4.323
© 2018 The Korean Nutrition Society
Anti-inflammatory activities of Scolopendra subspinipes mutilans in RAW 264.7 cells
Jae Hyeon Park and Sun Ryung Lee
Department of Biology, Jeju National University, Jeju 63243, Korea.

To whom correspondence should be addressed. tel: +82-64-754-3522, Email: srlee@jejunu.ac.kr
Received July 24, 2018; Revised July 26, 2018; Accepted August 01, 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

The dried body of Scolopendra subspinipes mutilans has long been used as a traditional Korean medicinal food, but little is known about its mechanisms of action. In this study, we investigated the anti-inflammatory activities of Scolopendra subspinipes mutilans and possible mechanisms in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.

Methods

Cytotoxicity of Scolopendra subspinipes mutilans extract (SSME) was measured by MTT assay, anti-inflammatory activities were analyzed by nitric oxide (NO) production, the expression of inducible NO synthase (iNOS) and the mRNA level of pro-inflammatory cytokines such as interleukin-1β (IL-1β) and interleukin-6 (IL-6). Nuclear translocation of nuclear factor-kappa B (NF-κB) p65 subunit and degradation of inhibitory kappa B (IκB) were examined by western blot.

Results

SSME inhibited LPS-induced NO production and iNOS expression without cytotoxicity. Up-regulation of LPS-induced pro-inflammatory cytokines, IL-1β and IL-6 was dose dependently attenuated by SSME. Exposure of pyrrolidine dithiocarbamate, an NF-κB specific inhibitor, accelerated the inhibitory effects of SSME on NO production and iNOS expression in LPS-stimulated cells. Moreover, translocation of NF-κB from the cytosol to the nucleus and degradation of IκB were decreased by treatment with SSME in LPS-induced cells.

Conclusion

These results suggest that the SSME might have the inhibitory effects on inflammation, partly through inhibition of the NF-κB signaling pathway.

Keywords: inflammation; nitric oxide; NF-κB; cytokine; Scolopendra subspinipes mutilans

Figures


Fig. 1
Cytotoxicity of Scolopendra subspinipes mutilans extract (SSME) in RAW 264.7 cells. The cells were treated with indicated concentration of SSE for 24 hrs. The data were presented as the mean ± SD (n=3, **p < 0.01 vs control).
Click for larger image


Fig. 2
Effect of Scolopendra subspinipes mutilans extract (SSME) on production of NO (A), expression of iNOS protein (B) and RNA level of pro-inflammatory cytokines (C) in LPS-stimulated RAW 264.7 cells. Cells were pre-treated with indicated concentration of SSME for 1 hr, and then stimulated with LPS for 24 hrs (A & B) or for 12 hrs (C). The data were presented as the mean ± SD (n = 3, **p < 0.01 vs LPS-treated group).
Click for larger image


Fig. 3
Inhibitory effect of Scolopendra subspinipes mutilans extract (SSME) on the NF-κB translocation to nucleus and IκB degradation (A) and NO production (B) in LPS-stimulated RAW 264.7 cells. Cells were pre-treated with indicated concentration of SSME and/or PDTC for 1 hr, and then stimulated with LPS for 75 min (A) or for 24 hrs (B). Different letters indicate significant differences among group at p < 0.01 as determined by Duncan's multiple range test.
Click for larger image

Notes

This research was supported by the 2017 scientific promotion program funded by Jeju National University.

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