Journal List > J Nutr Health > v.51(3) > 1097921

J Nutr Health. 2018 Jun;51(3):201-207. Korean.
Published online June 28, 2018.
© 2018 The Korean Nutrition Society
Anti-obesity and LDL-cholesterol lowering effects of silkworm hemolymph in C57BL/6N mice fed high fat diet
You Ree Nam, Young Eun Ko and Sun Yung Ly
Department of Food and Nutrition, Chungnam National University, Daejeon 34134, Korea.

To whom correspondence should be addressed. tel: +82-42-821-6838, Email:
Received April 16, 2018; Revised May 18, 2018; Accepted June 04, 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.



Obesity, a worldwide epidemic, is associated with insulin resistance, hyperlipidemia, hypertension, cardiovascular disease, and certain cancers. Many strategies, including natural alternative anti-obesity agents, are used widely to prevent obesity. This study examined the effects of silkworm hemolymph on the weight control of C57BL/6N mice fed with a high-fat diet.


The mice were divided into five groups: normal group (N), high-fat diet group (HFC), high-fat diet and silkworm hemolymph (at dose of 1 mL/kg BW (HFS-1), 5 mL/kg BW (HFS-5) and 10 mL/kg (HFS-10) for 12 weeks.


After 12 weeks treatment, the administration of silkworm hemolymph decreased the final body weight significantly along with a decrease in the weights of epididymal fat and total fat. The plasma LDL-cholesterol concentration was significantly lower in the HFS-1, HFS-5, and HFS-10 groups than in the HFC group. In addition, the leptin level of the HFS groups was significantly lower than those of the HFC group without a change in the plasma insulin concentration.


These findings suggest that the silkworm hemolymph may have the potential to prevent obesity.

Keywords: silkworm hemolymph; anti-obesity; LDL-cholesterol lowering effect


Table 1
Composition of experimental diets (%)
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Table 2
Changes in body weight of mice treated with high fat diet and silkworm hemolymph for 12 weeks
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Table 3
Weight gain and food efficiency ratio (FER) of mice treated with high fat diet and silkworm hemolymph for 12 weeks
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Table 4
Organ weight of mice treated with high fat diet and silkworm hemolymph for 12 weeks
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Table 5
Adipose tissue mass of mice treated with high fat diet and silkworm hemolymph for 12 weeks
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Table 6
AST and ALT activities in plasma of mice treated with high fat diet and silkworm hemolymph for 12 weeks
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Table 7
Plasma lipid profiles of mice treated with high fat diet and silkworm for 12 weeks
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Table 8
Plasma insulin and leptin levels of mice treated with high fat diet and silkworm hemolymph for 12 weeks
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