The India ink was diluted with normal saline solution and injected to note its intrinsic effect. The influence on sedimentation velocity (S.V.) of red blood cells after the injection of saline solution has been noted by many investigators. However Otani (86), Hotachi (35) found no change on the S. V. after saline injection while Ishiwari (41) observed that the injection of large doses of saline solution accelerate the S. V. of red cells.

The normal saline was injected daily for six days (5 cc per K. G. body weight intravenously once a day), [Table 1 and 2].

Thus no effect of the S. V. of red cells was noted after the injection of normal saline solution.

Variation of the S. V. of red cells during manipulation has been proved by many investigators. Thus Miwa(67) found the accelerated speed of S. V. of red cells in the artery when compared with the vein.

Otani (95) found increased velocity of sedimentation rate when blood was withrawn, while Araki (1) and Hotacni (35) noted no effect on the S. V. of red cells after withrawal of blood several times with 4 to 7 days. Takashina (112) found increased rate of sedimentation when about 7 cc blood was withrawn daily, and Takebayashi (111), Shibayama (110), Okamura (93) also found a relationship between the S. V. of red cells and quantity of blood withdrawn.

Blood was withdrawn at variable intervals and results are recorded in table 3 and 4.

There are no special changes on the S. V. of red cells, after experimental venepuncture in normal rabbits as table 3 and 4 show.

Many reports have appeared on the relationship between the S. V. of red cell and function of the thyroid gland.

Thus Trifon (115) noted the increase of S. V. when a diet containing thyroid substance was given while Bonilla and Moya (12) studied S. V. before and after thyroidectomy. Westergren (129) believes that the thyroid exerts a definite influence upon the S. V. of erythrocytes. Vasaturo (134) has investigated the sedimentation time in thyroidectomized, thyro-para- thyroidectomized animals and Wakata(130) has noted that the sedimentation velocity was accelerated by thyro-thymo-orchidectomy for at least 40 days. Tsunashima (155) reported that the S. V. of red cells decreased by thyroidectomy was later increased by administration of thyroid extract, but the rate of globulin and albumin does not stimulated. Takebayashi (111) noted no effect of S. V. of red blood cells under such conditions. Mensch (81) reported rapid S. V. in Basedow’s disease, and Sugano (107) also reported decreased volume of red cells in Basedow’s disease. Heing, Taterka, Goldmann (28) have studied rapid S. V. in Basedow’s disease. Yun (144) also studied the influence of thyroidectomy upon erythrocyte sedimentotion without any definite results.

The results are recorded in the following tables 8, 9, 10.

In my study, also there was no influence of thyroidectomy upon sedimentation of red blood cells.

The results are expressed in the following tables 11, 12, 13.

Thus thyroidectomy after blockade has no definite effect upon the erythrocyte S. V.

Thyreoglandol was given at the rate of 0.5 cc per k.g. and the results are recorded in the following tables 14, 15.

The above tables show no relation.

Thyreoglandol also was given after blockade and results are recorded in the following tables 16, 17.

Thus, there was no special influence on S. V. of red blood cells after thyroidectomy or thyroid medication.

Since Collip (14) succeded in isolating the parathyroid hormone many articles by many investigators have appeared and it has been clearly shown that there is a relationship between calcium metabolism and this substances.

Bomsker (11) reported that two different action on the blood calcium, Oshima (91) also reported on the subject. Zimmerman (143) noted absence of any effect on the calcium and coagulation time even after parathormone was injected. Bonilla and Moya (12) and Vasaturo (134) noted increased S. V. of red cells after parathyroidectomy, but Vasaturo (134) noted decreased S. V. of red cells after thyroparathyroidectomy. Yun(144) reported increased S. V. of red cells after parathyroidectomy.

Both parathyroid gland 2 out of 4 were removed from normal rabbits, and the results are recorded in the following tables 18, 19, 20.

As shown in above tables, the S. V. of red cells was increased.

The results are shown in the following tables 21, 22.

The rate of sedimentation was increased.

Parathormone was given intravenously at the rate of 1 to 5 units per k.g. and the results are noted in the following tables 23, 24, 25.

There was no special effect on the sedimentation velocity of red blood cells after the injection of parathormone.

Parathormone was given as in number 3 and the results are noted in the following tables 26, 27.

I found that there were no effect of parathormone injection on the red cell sedimentation after blockade.

The spleen has only recently been recognized as an organ of internal secretion. The relationship betweeu[n] the S. V. of erythrocytes and spleen function has been reported by many investigators. Kosawa reported increased fibrinogen content of the blood after splenectomy. Kame (154) noted increased globulin and decreased albumin in blood after splenectomy. Takashima (112) and Kitaoka (60) have reported on the blood coagulation time. Sakai (105), Shibayama (110), Tsunashima (155), Matsuhara (80), Seiffert (99) reported rapid sedimentation rate of erythrocytes after splenectomy, while no effect upon the S. V. of red cells was noted after splenectomy.

The results are recorded in the following tables 28, 29.

I found a rather more rapid sedimentation rate after splenectomy in normal rabbits.

The results are shown in the following tables 30, 31.

As the tables show the S. V. of erythrocytes was augmented.

2% spleen emulsion was injected intravenously at the rate of 1.0 cc per k.g. The results are recorded in the following tables 32, 33.

There are no special changes in the S. V. of red cells after the injection of spleen emulsion in normal rabbits.

The results are recorded in the following tables 34, 35.

The injection of spleen emulsion after blockade showed no special effect.

Since Addison reported in 1855 the syndrome known as Addison’s disease, and Takamine succeeded in 1901 in isolating adrenalin, many investigators have reported on this subject. The relationship between the S. V. of red cells and adrenalin gland hormones have been investigated by such workers as Seuderi, Morros, Sarda and Gaston who studied the sedimentation time in epilepsy, and Takebayashi (111) Ishiwari (41), Okamura (93) who studied the rapid sedimentation of erythrocytes after injection of adrenalin. Watanabe (131132) however reported no influence upon the sedimentation time.

Interenin by intravenous injection, was used at the rate of 1 cc per k.g. of body weight.

My results are given in the following tables 36, 37.

In the rabbits receiving interenin intravenously, augmentation of the velocity was noted.

The results are recorded in the following tables 38, 39.

There is no influence upon the sedimentation time due to interenin injection.

1: 1000 adrenalin solution (Sankyo) was injected intravenously at the rate of 0.5 cc per k.g. of body weight.

The following tables 40, 41 show the results.

The S. V. of red cells is decreased, but not markedly.

The following tables 42, 43 show my realts [results].

The S. V. of red cells is increased after the injection of Indian ink, but is decreased after the injection of adrenalin.

Since Banting discovered insulin in 1923, many investigations have been reported on the subject, especially on the relatiohship between insulin and blood sugar. Kanemori (52) repored decreased S. V. of red cells during convulsions due to insulin. Jindo (145) reported decrease after removal of the pancreas and increase after the injection of insulin. Pico- Francesche and Negrete (20) reported both increase of S. V. of red cells and lower blood sugar. Martens (79) recorded a relation betwren S. V. of red cells and necrosis of the pancreas.

Insulin (Lilly) was used at the rate of 1-5 units per k.g. and the resulis are recorded in the following tables 44, 45.

As the tables show, the velocity was decreased but not markedly.

Insulin was given at the rate of 1-5 units per k.g. after blockade and the results are recorded in the following tables 46, 47.

As shown in the tables the velocity first decreased but later increased.

Since Fahraeus (21) reported in 1918 that the velocity of sedimentation is increased in the pregnant woman as compared witn the nonpregnant woman, many investigators have reported a relation between sex and the sedimentation rate. Marukami (72), Kimura (51), Ryan and Johnson (97), Ogasahara (89), Takebayashi (111), Trifon(115) reported decrease of S.V. of red cells after ovariotomy and Ogasahara (89) reported increase of S. V. of red cells but it was uncertain as to whether the reaction is entirely due to luteohormone. Takebayashi (111) reported no special influence on the S. V. of red cells after ovariotomy, except a slight increase of S. V. of red cells for a few days. Uta (124)(129) reported no effect of ovarian hormone on the S. V. of red cells and the results are not the same when the urine of pregnant women was injected. Wakata (130) reported increase of sedimentation rate after orchidectomy but was uncertain as to whether the reaction was actually due to the operation and it was most reported by thyreo-thymectomy. Trifon (115) also reported increase of velocity after orchidectomy. Vasaturo (134) reported no definite effect on the sedimentation rate. Kanemori (52) reported increased velocity after orchidectomy but no change at all after the injection of the testicular hormone. Takebayashi (111) reported no influence of orchidectomy. Yun (144) reported increased velocity after orchidectomy.

Both testicles were removed. The results are recorded in the following tables 48, 49, 50.

As the tables show, the velocity was increased after castration.

Both testicles were removed after blockade, and the results are recorded in the following tables 51, 52, 53.

As shown in the tables, the velocity is also increased by castration.

The testiglandol was injected intravenously at the rate of 1 cc per k.g. and the results are shown in the following tables 54, 55.

Thus, the velocity is also decreased by injection of testiglandol.

The results are recordod [recorded] in the following tables 56, 57.

There was no influence due to testiglandol on the velocity after blockade.

The Thomas-Zeiss’ method and Sahli’s method were used. The following table gives my results [Table 66].

As shown in the table in every case the white cell count inceeased and the red cell and hemoglobin decreased after blockade.

Hedin-Koeppe’s method and Hess' Viscosimeter were used and

0. 58%-0.32% of salt solution used for testing fragility of the erythrocytes, and the series of saline solutions varied 0.02%. The results are recorded in the following table 67.

The results recorded, show that the blood volume decreased after blockade and there was no positive effect on the sedimentation velocity. There was also no noticiable effect on the fragility of the rod blood cells after blockade.

Abbes refraktometer was used for this work. The results are recorded in the following table 68.

As shown by the table, the globulin, albumin and content show no special relation with the S. V. of red cells, and fibrinogen content was increased in them after blockade.

Kramer-Tisdal’s method for calcium and Sachett’s modification of Bloor’s method for cholesterol, Hagedorn and Jensen’s method for sugar were used. The results are recorded in the following table 69.

As the table shows the serum calcium increased after blockade and the blood sugar also increased. There was no definite change in the blood cholesterol.