Journal List > J Nutr Health > v.50(1) > 1081484

J Nutr Health. 2017 Feb;50(1):10-24. Korean.
Published online February 28, 2017.  https://doi.org/10.4163/jnh.2017.50.1.10
© 2017 The Korean Nutrition Society
Effects of lymphocyte DNA damage levels in Korean plant food groups and Korean diet regarding to glutathione S-transferase M1 and T1 polymorphisms
Hyun-A Kim, Min-Young Lee and Myung-Hee Kang
Department of Food & Nutrition, Daedeok Valley Campus, Hannam University, Daejeon 34054, Korea.

To whom correspondence should be addressed. tel: +82-42-629-8791, Email: mhkang@hnu.kr
Received January 13, 2017; Revised February 06, 2017; Accepted February 10, 2017.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

GST (glutathione S-transferase) M1 and T1 gene polymorphisms are known to affect antioxidant levels. This study was carried out to evaluate genetic susceptibility by measuring the effect of DNA damage reduction in the Korean diet by vegetable food according to GST gene polymorphisms using the ex vivo method with human lymphocytes.

Methods

Vegetable foods in the Korean diet based the results of the KNHANES V-2 (2011) were classified into 10 food groups. A total of 84 foods, which constituted more than 1% of the total intake in each food group, were finally designated as a vegetable food in the Korean diet. The Korean diet applied in this study is the standard one-week meals for Koreans (2,000 Kcal/day) suggested by the 2010 Dietary Reference Intakes for Koreans. Ex vivo DNA damage in human lymphocytes was assessed using comet assay.

Results

In the Korean food group, the DNA damage protective effect of GSTM1 and GSTT1 was found to be greater in mutant type and wild-type, respectively. and the DNA damage protective effect according to the combined genotype of GSTM1 and GSTT1 was different depending on the food group. On the other hand, in Korean Diet, the DNA damage protective effect appeared to be larger in GSTM1 wild-type than in mutant type and was found to not be affected by GSTT1 genotype.

Conclusion

These results can be used as basic data to demonstrate the superiority of the antioxidant function of Korean dietary patterns and food groups. Furthermore, it may be a starting point to begin research on customized antioxidant nutrition according to individual genes.

Keywords: DNA damage; GST polymorphism; Korean plant food groups; Korean Diet; Comet assay

Figures


Fig. 1
Relative score of lymphocyte DNA damage of Korean plant food groups at 50 µg/ml concentration according to the GSTM1 genotype. *p < 0.05, t-test. Total: mixture of 10 Korean plant food groups.
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Fig. 2
Relative score of lymphocyte DNA damage of Korean plant food groups at 50 µg/ml concentration according to the GSTT1 genotype. * p < 0.05, **p < 0.01, ***p < 0.001, t-test. Total: mixture of 10 Korean plant food groups.
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Fig. 3
Relative score of lymphocyte DNA damage of Korean diet at 250 µg/ml concentration according to the GSTM1 and GSTT1 genotype. *p < 0.05, ***p < 0.001. NS: not significant, t-test
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Fig. 4
The effect of Korean diet pre-treatment on H2O2-induced DNA damage in human lymphocytes, classified according to GST genotype. Different letters are significantly different among GST genotypes by Duncan's multiple range test.
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Tables


Table 1
Intake of plant foods and oils in the Korean diet (KNHANES V-2, 2011)
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Table 2
Normal dietary pattern of Korean diet (a week_2,000 kcal/day)1)
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Table 3
Primer sequences used in the PCR reactions
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Table 4
The effect of Korean plant food group pre-treatment on H2O2-induced DNA damages in human lymphocytes, classified according to GST genotype1)
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References
1. Ministry of Health and Welfare, Korea Centers for Disease Control and Prevention. Korea Health Statistics 2015: Korea National Health and Nutrition Examination Survey (KNHANES VI-3).
2. Lee HS, Cho YH, Park J, Shin HR, Sung MK. Dietary intake of phytonutrients in relation to fruit and vegetable consumption in Korea. J Acad Nutr Diet 2013;113(9):1194–1199.
3. Lee JY. In: A study on planning the policy for the globalization of Korean food [dissertation]. Seoul: Chung-Ang University; 2009.
4. Omenn GS, Goodman GE, Thornquist MD, Balmes J, Cullen MR, Glass A, Keogh JP, Meyskens FL Jr, Valanis B, Williams JH Jr, Barnhart S, Cherniack MG, Brodkin CA, Hammar S. Risk factors for lung cancer and for intervention effects in CARET, the Beta-Carotene and Retinol Efficacy Trial. J Natl Cancer Inst 1996;88(21):1550–1559.
5. Park CH, Kim KH, Yook HS. Comparison of antioxidant and antimicrobial activities of bracken (Pteridium aquilinum Kuhn) according to cooking methods. Korean J Food Nutr 2014;27(3):348–357.
6. Lee MY, Han JH, Kang MH. Protective effect of Korean diet food groups on lymphocyte DNA damage and contribution of each food group to total dietary antioxidant capacity (TDAC). J Nutr Health 2016;49(5):277–287.
7. Lee MY, Kim HA, Kang MH. Comparison of lymphocyte DNA damage levels and total antioxidant capacity in Korean and American diet. Nutr Res Pract 2017;11(1):33.
8. Park YK, Park E, Kim JS, Kang MH. Daily grape juice consumption reduces oxidative DNA damage and plasma free radical levels in healthy Koreans. Mutat Res 2003;529(1-2):77–86.
9. Pool-Zobel BL, Bub A, Müller H, Wollowski I, Rechkemmer G. Consumption of vegetables reduces genetic damage in humans: first results of a human intervention trial with carotenoid-rich foods. Carcinogenesis 1997;18(9):1847–1850.
10. Sinha R, Caporaso N. Diet, genetic susceptibility and human cancer etiology. J Nutr 1999;129 2S Suppl:556S–559S.
11. Cho MR, Han JH, Lee HJ, Park YK, Kang MH. Purple grape juice supplementation in smokers and antioxidant status according to different types of GST polymorphisms. J Clin Biochem Nutr 2015;56(1):49–56.
12. Cho HJ, Lee SY, Ki CS, Kim JW. GSTM1, GSTT1 and GSTP1 polymorphisms in the Korean population. J Korean Med Sci 2005;20(6):1089–1092.
13. Rebbeck TR. Molecular epidemiology of the human glutathione S-transferase genotypes GSTM1 and GSTT1 in cancer susceptibility. Cancer Epidemiol Biomarkers Prev 1997;6(9):733–743.
14. Saura-Calixto F, Goñi I. Antioxidant capacity of the Spanish Mediterranean diet. Food Chem 2006;94(3):442–447.
15. Han JH, Lee HJ, Cho MR, Chang N, Kim Y, Oh SY, Kang MH. Total antioxidant capacity of the Korean diet. Nutr Res Pract 2014;8(2):183–191.
16. Hofmann T, Kuhnert A, Schubert A, Gill C, Rowland IR, Pool-Zobel BL, Glei M. Modulation of detoxification enzymes by watercress: in vitro and in vivo investigations in human peripheral blood cells. Eur J Nutr 2009;48(8):483–491.
17. Lampe JW, Chen C, Li S, Prunty J, Grate MT, Meehan DE, Barale KV, Dightman DA, Feng Z, Potter JD. Modulation of human glutathione S-transferases by botanically defined vegetable diets. Cancer Epidemiol Biomarkers Prev 2000;9(8):787–793.
18. Kim SJ, Kim MG, Kim KS, Song JS, Yim SV, Chung JH. Impact of glutathione S-transferase M1 and T1 gene polymorphisms on the smoking-related coronary artery disease. J Korean Med Sci 2008;23(3):365–372.
19. Yu Y, Song Y. Three clustering patterns among metabolic syndrome risk factors and their associations with dietary factors in Korean adolescents: based on the Korea National Health and Nutrition Examination Survey of 2007-2010. Nutr Res Pract 2015;9(2):199–206.
20. Woo HD, Shin A, Kim J. Dietary patterns of Korean adults and the prevalence of metabolic syndrome: a cross-sectional study. PLoS One 2014;9(11):e111593.
21. The Korean Nutrition Society. Dietary referece intakes for Koreans. 1st rev. ed. Seoul: The Korean Nutrition Society; 2010.
22. Lee SG, Oh SC, Jang JS. Antioxidant activities of citrus unshiu extracts obtained from different solvents. Korean J Food Nutr 2015;28(3):458–464.
23. Pemble S, Schroeder KR, Spencer SR, Meyer DJ, Hallier E, Bolt HM, Ketterer B, Taylor JB. Human glutathione S-transferase theta (GSTT1): cDNA cloning and the characterization of a genetic polymorphism. Biochem J 1994;300(Pt 1):271–276.
24. Bell DA, Taylor JA, Paulson DF, Robertson CN, Mohler JL, Lucier GW. Genetic risk and carcinogen exposure: a common inherited defect of the carcinogen-metabolism gene glutathione S-transferase M1 (GSTM1) that increases susceptibility to bladder cancer. J Natl Cancer Inst 1993;85(14):1159–1164.
25. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988;175(1):184–191.
26. Riso P, Martini D, Møller P, Loft S, Bonacina G, Moro M, Porrini M. DNA damage and repair activity after broccoli intake in young healthy smokers. Mutagenesis 2010;25(6):595–602.
27. Marotta F, Weksler M, Naito Y, Yoshida C, Yoshioka M, Marandola P. Nutraceutical supplementation: effect of a fermented papaya preparation on redox status and DNA damage in healthy elderly individuals and relationship with GSTM1 genotype: a randomized, placebo-controlled, cross-over study. Ann N Y Acad Sci 2006;1067(1):400–407.
28. Gasper AV, Al-Janobi A, Smith JA, Bacon JR, Fortun P, Atherton C, Taylor MA, Hawkey CJ, Barrett DA, Mithen RF. Glutathione S-transferase M1 polymorphism and metabolism of sulforaphane from standard and high-glucosinolate broccoli. Am J Clin Nutr 2005;82(6):1283–1291.
29. Lampe JW. Interindividual differences in response to plant-based diets: implications for cancer risk. Am J Clin Nutr 2009;89(5):1553S–1557S.
30. Yuan L, Ma W, Liu J, Meng L, Liu J, Li S, Han J, Liu Q, Feng L, Wang C, Xiao R. Effects of GSTM1/GSTT1 gene polymorphism and fruit & vegetable consumption on antioxidant biomarkers and cognitive function in the elderly: a community based cross-sectional study. PLoS One 2014;9(11):e113588.
31. Hakim IA, Harris RB, Chow HH, Dean M, Brown S, Ali IU. Effect of a 4-month tea intervention on oxidative DNA damage among heavy smokers: role of glutathione S-transferase genotypes. Cancer Epidemiol Biomarkers Prev 2004;13(2):242–249.
32. Tang JJ, Wang MW, Jia EZ, Yan JJ, Wang QM, Zhu J, Yang ZJ, Lu X, Wang LS. The common variant in the GSTM1 and GSTT1 genes is related to markers of oxidative stress and inflammation in patients with coronary artery disease: a case-only study. Mol Biol Rep 2010;37(1):405–410.
33. Wilms LC, Boots AW, de Boer VC, Maas LM, Pachen DM, Gottschalk RW, Ketelslegers HB, Godschalk RW, Haenen GR, van Schooten FJ, Kleinjans JC. Impact of multiple genetic polymorphisms on effects of a 4-week blueberry juice intervention on ex vivo induced lymphocytic DNA damage in human volunteers. Carcinogenesis 2007;28(8):1800–1806.
34. Wilms LC, Claughton TA, de Kok TM, Kleinjans JC. GSTM1 and GSTT1 polymorphism influences protection against induced oxidative DNA damage by quercetin and ascorbic acid in human lymphocytes in vitro. Food Chem Toxicol 2007;45(12):2592–2596.
35. Valerio LG Jr, Kepa JK, Pickwell GV, Quattrochi LC. Induction of human NAD(P)H:quinone oxidoreductase (NQO1) gene expression by the flavonol quercetin. Toxicol Lett 2001;119(1):49–57.
36. Seow A, Shi CY, Chung FL, Jiao D, Hankin JH, Lee HP, Coetzee GA, Yu MC. Urinary total isothiocyanate (ITC) in a population-based sample of middle-aged and older Chinese in Singapore: relationship with dietary total ITC and glutathione S-transferase M1/T1/P1 genotypes. Cancer Epidemiol Biomarkers Prev 1998;7(9):775–781.
37. Visanji JM, Duthie SJ, Pirie L, Thompson DG, Padfield PJ. Dietary isothiocyanates inhibit Caco-2 cell proliferation and induce G2/M phase cell cycle arrest, DNA damage, and G2/M checkpoint activation. J Nutr 2004;134(11):3121–3126.
38. Pool-Zobel B, Veeriah S, Böhmer FD. Modulation of xenobiotic metabolising enzymes by anticarcinogens -- focus on glutathione S-transferases and their role as targets of dietary chemoprevention in colorectal carcinogenesis. Mutat Res 2005;591(1-2):74–92.
39. Yuan L, Zhang L, Ma W, Zhou X, Ji J, Li N, Xiao R. Glutathione S-transferase M1 and T1 gene polymorphisms with consumption of high fruit-juice and vegetable diet affect antioxidant capacity in healthy adults. Nutrition 2013;29(7-8):965–971.
40. Zhao B, Seow A, Lee EJ, Poh WT, Teh M, Eng P, Wang YT, Tan WC, Yu MC, Lee HP. Dietary isothiocyanates, glutathione S-transferase -M1, -T1 polymorphisms and lung cancer risk among Chinese women in Singapore. Cancer Epidemiol Biomarkers Prev 2001;10(10):1063–1067.
41. Schroeder N, Park YH, Kang MS, Kim Y, Ha GK, Kim HR, Yates AA, Caballero B. A randomized trial on the effects of 2010 Dietary Guidelines for Americans and Korean diet patterns on cardiovascular risk factors in overweight and obese adults. J Acad Nutr Diet 2015;115(7):1083–1092.
42. Zamora-Ros R, Serafini M, Estruch R, Lamuela-Raventós RM, Martínez-González MA, Salas-Salvadó J, Fiol M, Lapetra J, Arós F, Covas MI, Andres-Lacueva C. PREDIMED Study Investigators. Mediterranean diet and non enzymatic antioxidant capacity in the PREDIMED study: evidence for a mechanism of antioxidant tuning. Nutr Metab Cardiovasc Dis 2013;23(12):1167–1174.
43. Estruch R, Ros E, Salas-Salvadó J, Covas MI, Corella D, Arós F, Gómez-Gracia E, Ruiz-Gutiérrez V, Fiol M, Lapetra J, Lamuela-Raventos RM, Serra-Majem L, Pintó X, Basora J, Muñoz MA, Sorlí JV, Martínez JA, Martínez-González MA. PREDIMED Study Investigators. Primary prevention of cardiovascular disease with a Mediterranean diet. N Engl J Med 2013;368(14):1279–1290.
44. Chung J, Kwon SO, Ahn H, Hwang H, Hong SJ, Oh SY. Association between dietary patterns and atopic dermatitis in relation to GSTM1 and GSTT1 polymorphisms in young children. Nutrients 2015;7(11):9440–9452.