Role of zinc for calcification inhibitor protein in vascular smooth muscle cell plaque formation

Mee-Young Shin; In-Sook Kwun

doi:10.4163/jnh.2016.49.1.59

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Shin and Kwun: Role of zinc for calcification inhibitor protein in vascular smooth muscle cell plaque formation

Note

Journal of Nutrition and Health 2016; 49(1): 59-62.

Published online: 29 February 2016

DOI: https://doi.org/10.4163/jnh.2016.49.1.59

Role of zinc for calcification inhibitor protein in vascular smooth muscle cell plaque formation

Mee-Young Shin, In-Sook Kwun

Department of Food science and Nutrition, Andong National University, Andong 36729, Korea.

To whom correspondence should be addressed. tel: +82-54-820-5917, iskwun@andong.ac.kr

Received 1 February 2016 Revised 16 February 2016 Accepted 17 February 2016

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose

Zinc, a biomineral present within and outside cells, manages various cellular mechanisms. In this study, we examined whether zinc was involved in vascular smooth muscle cell (VSMC) calcification via regulation of calcification inhibitor protein, osteopontin (OPN).

Methods

Rat aorta cell line (A7r5 cells) and primary vascular smooth muscle cells (pVSMCs) from rat aorta were cultured with phosphate (1-5 mM) and zinc (0-15 µM) as appropriate, along with osteoblasts (MC3T3-E1) as control. The cells were then stained for Ca and P deposition for calcification examination as well as osteopontin expression as calcification inhibitor protein was measured.

Results

Both Ca and phosphate deposition increased as the addition of phosphate increased. In the same manner, the expression of osteopontin was upregulated as the addition of phosphate increased in both cell types. When zinc was added, Ca and P deposition decreased in VSMCs, while it increased in osteoblasts.

Conclusion

The results imply that zinc may prevent VSMC calcification by stimulating calcification inhibitor protein OPN synthesis in VSMCs.

Keywords: vascular smooth muscle cells, calcification, osteopontin, osteoblasts

Figures and Tables

Fig. 1

The addition of phosphate induced Ca (A) and P (B) deposition in vascular smooth muscle cells (pVSMCs and A7r5 cells) as well as in osteoblasts (MC3T3-E1 cells). Cells were cultured with the designated phosphate levels for 12 days and stained for Ca (A, in red) and P (B, in black) deposition.

Fig. 2

Calcification inhibitor protein, osteopontin, upregulated as the addition of P in creased in both VSMCs (A, A7r5) and osteoblasts (B, MC3T3-E1). Cells were cultured with the designated P level for 12 days.

Fig. 3

The addition of zinc prevented Ca and P accumulation in vascular smooth muscle cell A7r5 under calcified condition (A. at 3 and 5 mM P addition), while zinc stimulated Ca and P accumulation in osteoblasts. Cells were cultured with the designated Zn and phosphate level for 12 days.

Notes

This work was supported by 2012 ANU Industry-Academic Research Grant from Andong National University.

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