Journal List > J Nutr Health > v.48(6) > 1081418

J Nutr Health. 2015 Dec;48(6):468-475. Korean.
Published online December 31, 2015.  https://doi.org/10.4163/jnh.2015.48.6.468
© 2015 The Korean Nutrition Society
Oxidative damage biomarker levels according to Mn-SOD and GST gene polymorphisms in preschool children
You-kyung Shin,1,2 Ji-Won Choi,1 Se-Young Oh,1 and Jayong Chung1
1Department of Food and Nutrition, Kyung Hee University, Seoul 02447, Korea.
2R&D center, Seoul Dairy Cooperative, Ansan, Kyunggi 15407, Korea.

To whom correspondence should be addressed. tel: +82-2-961-0977, Email: jchung@khu.ac.kr
Received September 09, 2015; Revised September 25, 2015; Accepted October 16, 2015.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

Genetic polymorphisms in antioxidant defense and detoxification genes may modulate the levels of oxidative stress biomarkers.

Methods

A total of 301 healthy preschool-aged children in the Seoul and Kyung-gi areas were recruited. DNA was extracted from blood for genotyping of manganese superoxide dismutase (Mn-SOD) Val16Ala, glutathione S-transferase (GST) P1 Ile105Val, GSTT1 present/null, and GSTM1 present/null polymorphisms by PCR-restriction fragment length polymorphism or multiplex PCR analyses. In addition to a questionnaire survey, the levels of urinary 8- hydroxyl-2-deoxiguanosine (8-OHdG) and plasma malondialdehyde (MDA) were measured by ELISA.

Results

Significantly higher urinary 8-OHdG concentrations were observed in GSTP1 Ile/Val + Val/Val genotype (p = 0.030), and tended to be higher in Mn-SOD Val/Val genotype (p = 0.065). On the other hand, exposure to environmental tobacco smoking (ETS) and interaction between ETS and gene polymorphisms did not significantly influence either urinary 8-OHdG concentrations or serum MDA.

Conclusion

Based on our findings, GSTP1 Ile/Val gene polymorphisms might modulate the levels of oxidative stress biomarkers in healthy preschool children.

Keywords: Genetic polymorphism; Mn-superoxide dismutase; glutathione S-transferase; 8-hydroyxyl-2-deoxyguanosine

Figures


Fig. 1
The levels of urinary 8-hydroxyl-2-deoxyguanosine (8-OHdG) according to exposure to environmental tobacco smoking (ETS) and gene polymorphisms. [(A) Mn-SOD, (B) GSTP1, (C) GSTT1, and (D) GSTM1] Data were analyzed using GLM models adjusted for age, sex, and nutrient supplement intake.
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Tables


Table 1
General characteristics of the study population
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Table 2
Genotype distributions and allelic frequencies for Mn- SOD, GSTP1, GSTT1, and GSTM1 gene polymorphisms in the study population
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Table 3
Concentrations of oxidative stress markers according to polymorphisms
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Notes

This work was supported by grants from the Basic Science Research Program of the National Research Foundation (NRF2013 R1A1A2007613).

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