Journal List > J Nutr Health > v.47(4) > 1081341

J Nutr Health. 2014 Aug;47(4):229-235. Korean.
Published online August 31, 2014.
© 2014 The Korean Nutrition Society
Effects of xylooligosaccharide-sugar mixture on glycemic index (GI) and blood glucose response in healthy adults
Myungok Kyung,1,** HanSaem Choe,2,** Sangwon Jung,1 Kyungsun Lee,1 SungEun Jo,1 Sheungwoo Seo,1 Keunbum Choe,1 Chang-Kun Yang,3 Sang-Ho Yoo,4 and Yuri Kim2
1R&D Center, TS Corporation, Incheon 400-201, Korea.
2Department of Nutritional Science and Food Management, Ewha Womans University, Seoul 120-750, Korea.
3TS Food & System, Seoul 135-921, Korea.
4Department of Food Science & Technology, BK21 Plus Team, and Carbohydrate Bioproduct Research Center, Sejong University, Seoul 143-747, Korea.

To whom correspondence should be addressed. tel: +82-2-3277-4485, Email:

**Equal contribution as the first author.

Received May 10, 2014; Revised June 10, 2014; Accepted August 01, 2014.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.



The objective of this study was to investigate the effects of xyloologosaccharide (XOS)-sugar mixture on glycemic index (GI) and blood glucose in human subjects.


Randomized double-blind cross-over studies were conducted to examine the effect of sucrose with 14% xyloologosaccharide powder (Xylo 14) and sucrose with 20% xylooligosaccharide powder (Xylo 20) on GI and postprandial glucose response at 15, 30, 45, 60, 90, and 120 min.


GIs of Xylo 14 and Xylo 20 were 60.0 ± 23.5 classified within medium GI range, and 54.3 ± 17.7 within low GI range, respectively. Xylo 14 and Xylo 20 showed significantly lower area under the glucose curve (AUC) for 0-15 min (p = 0.0113), 0-30 min (p = 0.0004), 0-45 min (p < 0.0001), 0-60 min (p < 0.0001), 0-90 min (p < 0.0001), and 0-120 min (p = 0.0001). In particular, compared with glucose, the blood glucose levels of Xylo 14 and Xylo 20 were significantly lower at every time point between 15 and 120 min.


The results of this study suggested that Xylo 14 and Xylo 20 had an acute suppressive effect on GI and the postprandial glucose surge.

Keywords: xylooligosaccharide (XOS); sugar; GI (glycemic index); area under the curve (AUC); blood glucose


Fig. 1
Mean blood glucose responses after administration of control food (glucose) and test food (Xylo 14 and Xylo 20). Each value is the mean ± SD. Time points with a p-value indicate a significant difference between groups. Xylo 14, sucrose with 14% xylooligosaccharide powder; Xylo 20, sucrose with 20% xylooligosaccharide powder.
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Table 1
Test food compositions in the clinical trial
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Table 2
Baseline characteristics of the subjects in the clinical trial n = 11
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Table 3
Glycemic indices of Xylo 14 and Xylo 20 n = 11
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Table 4
Blood glucose areas under curve (AUC) in subjects after ingestion of Glucose, Xylo 14, and Xylo 20 n = 11
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This research was supported by High Value-added Food Technology Development Program (Project number: 313024-03-1-CG000), Mi-nistry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

1. Yoo HJ, Kim Y. A study on the characteristics of nutrient intake in metabolic syndrome subjects. Korean J Nutr 2008;41(6):510–517.
2. United States Department of Agriculture. Sugar: world markets and trade. Washington, D.C.: United States Department of Agriculture; 2013.
3. Bae YJ, Bak YK, Kim B, Kim MS, Lee JH, Sung MK. Coconut-derived D-xylose affects postprandial glucose and insulin responses in healthy individuals. Nutr Res Pract 2011;5(6):533–539.
4. Ferder L, Ferder MD, Inserra F. The role of high-fructose corn syrup in metabolic syndrome and hypertension. Curr Hypertens Rep 2010;12(2):105–112.
5. Yang Q. Gain weight by "going diet?" Artificial sweeteners and the neurobiology of sugar cravings: Neuroscience 2010. Yale J Biol Med 2010;83(2):101–108.
6. Swithers SE. Artificial sweeteners produce the counterintuitive effect of inducing metabolic derangements. Trends Endocrinol Metab 2013;24(9):431–441.
7. Prudel M, Davídková E, Davídek J, Kmínek M. Kinetics of decomposition of aspartame hydrochloride (Usal) in aqueous solutions. J Food Sci 1986;51(6):1393–1397.
8. Kim WJ, Chung NY. Effect of temperature and pH on thermal stability of aspartame. Korean J Food Sci Technol 1996;28(2):311–315.
9. Jenkins DJ, Wolever TM, Taylor RH, Barker H, Fielden H, Baldwin JM, Bowling AC, Newman HC, Jenkins AL, Goff DV. Glycemic index of foods: a physiological basis for carbohydrate exchange. Am J Clin Nutr 1981;34(3):362–366.
10. Willett W, Manson J, Liu S. Glycemic index, glycemic load, and risk of type 2 diabetes. Am J Clin Nutr 2002;76(1):274S–280S.
11. Brand-Miller JC, Holt SH, Pawlak DB, McMillan J. Glycemic index and obesity. Am J Clin Nutr 2002;76(1):281S–285S.
12. Miller JC. Importance of glycemic index in diabetes. Am J Clin Nutr 1994;59 3 Suppl:747S–752S.
13. Hodge AM, English DR, O'Dea K, Giles GG. Glycemic index and dietary fiber and the risk of type 2 diabetes. Diabetes Care 2004;27(11):2701–2706.
14. Park S, Woo MH, Choue R. Dietary quality and self-management status according to the glycemic control in the elderly with type 2 diabetes. Korean J Food Nutr 2008;21(4):530–535.
15. Brouns F, Bjorck I, Frayn KN, Gibbs AL, Lang V, Slama G, Wolever TM. Glycaemic index methodology. Nutr Res Rev 2005;18(1):145–171.
16. Krog-Mikkelsen I, Hels O, Tetens I, Holst JJ, Andersen JR, Bukhave K. The effects of L-arabinose on intestinal sucrase activity: dose-response studies in vitro and in humans. Am J Clin Nutr 2011;94(2):472–478.
17. Moon S, Lee K, Kyung M, Jung S, Park Y, Yang CK. Study on the proper D-xylose concentration in sugar mixture to reduce glycemic index (GI) value in the human clinical model. Korean J Food Nutr 2012;25(4):787–792.
18. Lee K, Moon S, Jung S, Park YJ, Yoon S, Choe K, Yang C. Glycemic index of sucrose with D-xylose (XF) in humans. Curr Top Nutraceutical Res 2013;11(1/2):35–40.
19. Mäkeläinen H, Juntunen M, Hasselwander O. Prebiotic potential of xylo-oligosaccharides. In: Charalampopoulos D, Rastall RA, editors. Prebiotics and Probiotics Science and Technology. New York: Springer Verlag; 2009. pp. 245-258.
20. Imaizumi K, Nakatsu Y, Sato M, Sedarnawati Y, Sugano M. Effects of xylooligosaccharides on blood glucose, serum and liver lipids and cecum short-chain fatty acids in diabetic rats. Agric Biol Chem 1991;55(1):199–205.
21. Ministry of Food and Drug Safety (KR). Xylooligosaccharide [Internet]. Cheongwon: Ministry of Food and Drug Safety; 2009 [cited 2014 Apr 21].
22. Crittenden R, Karppinen S, Ojanen S, Tenkanen M, Fagerström R, Mättö J, Saarela M, Mattila-Sandholm T, Poutanen K. In vitro fermentation of cereal dietary fibre carbohydrates by probiotic and intestinal bacteria. J Sci Food Agric 2002;82(8):781–789.
23. Wolever TM, Vorster HH, Björck I, Brand-Miller J, Brighenti F, Mann JI, Ramdath DD, Granfeldt Y, Holt S, Perry TL, Venter C, Xiaomei Wu. Determination of the glycaemic index of foods: interlaboratory study. Eur J Clin Nutr 2003;57(3):475–482.
24. NeoNutra Co., Ltd. Clinical study report (Protocol No.: Xy1 T). Randomized, open-label, single-dose and crossover clinical study to measure of glycemic index of XylooloigoSugar and Xylooligo Sweet on healthy adults. Seoul: NeoNutra Co., Ltd.; 2013.
25. Ministry of Food and Drug Safety (KR). Korean food standards codex [Internet]. Cheongwon: Ministry of Food and Drug Safety; 2013 [cited 2014 Jul 20].
26. Wolever TM, Jenkins DJ. The use of the glycemic index in predicting the blood glucose response to mixed meals. Am J Clin Nutr 1986;43(1):167–172.
27. Foster-Powell K, Holt SH, Brand-Miller JC. International table of glycemic index and glycemic load values: 2002. Am J Clin Nutr 2002;76(1):5–56.
28. Roberts SB. High-glycemic index foods, hunger, and obesity: is there a connection? Nutr Rev 2000;58(6):163–169.
29. Ludwig DS. Dietary glycemic index and obesity. J Nutr 2000;130 2S Suppl:280S–283S.
30. Oba S, Nagata C, Nakamura K, Fujii K, Kawachi T, Takatsuka N, Shimizu H. Dietary glycemic index, glycemic load, and intake of carbohydrate and rice in relation to risk of mortality from stroke and its subtypes in Japanese men and women. Metabolism 2010;59(11):1574–1582.
31. Shibanuma K, Degawa Y, Houda K. Determination of the transient period of the EIS complex and investigation of the suppression of blood glucose levels by L-arabinose in healthy adults. Eur J Nutr 2011;50(6):447–453.
32. Seri K, Sanai K, Matsuo N, Kawakubo K, Xue C, Inoue S. L-arabinose selectively inhibits intestinal sucrase in an uncompetitive manner and suppresses glycemic response after sucrose ingestion in animals. Metabolism 1996;45(11):1368–1374.
33. Preuss HG, Echard B, Bagchi D, Stohs S. Inhibition by natural dietary substances of gastrointestinal absorption of starch and sucrose in rats and pigs: 1. Acute studies. Int J Med Sci 2007;4(4):196–202.
34. Yuasa H, Kuno C, Watanabe J. Comparative assessment of D-xylose absorption between small intestine and large intestine. J Pharm Pharmacol 1997;49(1):26–29.
35. Kabel MA, Kortenoeven L, Schols HA, Voragen AG. In vitro fermentability of differently substituted xylo-oligosaccharides. J Agric Food Chem 2002;50(21):6205–6210.
36. Grootaert C, Delcour JA, Courtin CM, Broekaert WF, Verstraete W, Van de Wielea T. Microbial metabolism and prebiotic potency of arabinoxylan oligosaccharides in the human intestine. Trends Food Sci Technol 2007;18(2):64–71.
37. Livesey G, Tagami H. Interventions to lower the glycemic response to carbohydrate foods with a low-viscosity fiber (resistant maltodextrin): meta-analysis of randomized controlled trials. Am J Clin Nutr 2009;89(1):114–125.
38. Lee MY, Kim MK, Shin JG, Kim SD. Dietary effect of hemicellulose from soy fiber on blood glucose and cholesterol content in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 2004;33(7):1119–1125.
39. Park MY, Kim KH, Jeong KS, Kim HA. Effect of supplementation of dietary sea tangle on the renal oxidative stress in diabetic rats. Korean J Food Cult 2007;22(1):140–148.