Journal List > J Nutr Health > v.47(3) > 1081333

J Nutr Health. 2014 Jun;47(3):157-166. Korean.
Published online June 30, 2014.  https://doi.org/10.4163/jnh.2014.47.3.157
© 2014 The Korean Nutrition Society
Effect of stabilized rice bran-added high sucrose diet on glucose control in C57BL/6 mice
Seung-Min Lee,1 Mal-Shick Shin,1,2 and Young-Ran Heo1,2
1Department of Food and Nutrition, Chonnam National University, Gwangju 500-757, Korea.
2Human Ecology Research Institute, Chonnam National University, Gwangju 500-757, Korea.

To whom correspondence should be addressed. tel: +82-62-530-1338, Email: yrhuh@jnu.ac.kr
Received May 30, 2014; Revised June 22, 2014; Accepted June 23, 2014.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

Purpose

Rice bran is a byproduct of the hulling of rice and contains a variety of bioactive components. Various studies have reported on the antioxidative, anticancer, immune-enhancing, and hypocholesterolemic effects of rice bran. However, few studies about the physiological activity of stabilized rice bran supplement on dietary intake of sugars is limited. The aim of this study was to investigate the effect of stabilized rice bran supplement on blood glucose in C57BL/6 mice fed a high sucrose diet.

Methods

Animals were randomly divided into three groups respectively, and were fed a normal diet (ND group), a high sucrose diet (HSD group) or a high sucrose diet containing 20% stabilized rice bran (HSD-SRB group) for 12 weeks.

Results

In the oral glucose tolerance test (OGTT), after seven weeks of feeding on the experimental diets, a significantly lower result was observed for HSD-SRB than for HSD at 30 and 60 minutes after oral administration in glucose solution (2 g/kg body weight). The incremental area under the curve (IAUC) of HSD-SRB was significantly lower than that of HSD. After 12 weeks, fasting blood glucose level of HSD-SRB was significantly lower than that of HSD. No significant difference in the serum insulin level was observed between HSD and HSD-SRB. However, HOMA-IR was significantly decreased in HSD-SRB compared to HSD. In addition, HOMA β-cell was significantly increased in HSD-SRB com-pared to HSD. Triglyceride in liver of HSD-SRB was significantly lower than that of HSD.

Conclusions

Feeding diets con-taining 20% rice bran improved insulin resistance and insulin secretion by decreasing triglyceride in liver. Thus, rice bran has a positive effect on glycemic control. In addition, the results are expected to be utilized as a basis for human study and development of food products with added rice bran.

Keywords: rice bran; high sucrose diet; insulin resistance; blood glucose

Figures


Fig. 1
Effect of stabilized rice bran on blood glucose (A) and incremental area under the curve (B) after oral glucose tolerance test in C57BL/6 mice. Values are expressed as mean ± SE. abDifferent letters over the bars indicate that the mean values are significantly different (p < 0.05) by Duncan's multiple range test. ND: normal diet, HSD: high sucrose diet, HSD-SRB: high sucrose diet in which 20% dietary calorie nutrient (g) is replaced with stabilized rice bran.
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Fig. 2
Effect of stabilized rice bran on serum insulin concentration in C57BL/6 mice. Values are expressed as mean ± SE. ND: normal diet, HSD: high sucrose diet, HSD-SRB: high sucrose diet in which 20% dietary calorie nutrient (g) is replaced with stabilized rice bran.
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Fig. 3
Effect of stabilized rice bran on serum adiponectin concentration in C57BL/6 mice. Values are expressed as mean ± SE. abDifferent letters over the bars indicate that the mean values are significantly different (p < 0.05) by Duncan's multiple range test. ND: normal diet, HSD: high sucrose diet, HSD-SRB: high sucrose diet in which 20% dietary calorie nutrient (g) is replaced with stabilized rice bran.
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Fig. 4
Effect of stabilized rice bran on HOMA-IR (A) and HOMA β-cell (B) in C57BL/6 mice. Values are expressed as mean ± SE. abDifferent letters over the bars indicate that the mean values are significantly different (p < 0.05) by Duncan's multiple range test. ND: normal diet, HSD: high sucrose diet, HSD-SRB: high sucrose diet in which 20% dietary calorie nutrient (g) is replaced with stabilized rice bran.
Click for larger image


Fig. 5
Effect of stabilized rice bran on liver glycogen concentration in C57BL/6 mice. Values are expressed as mean ± SE. ND: normal diet, HSD: high sucrose diet, HSD-SRB: high sucrose diet in which 20% dietary calorie nutrient (g) is replaced with stabilized rice bran.
Click for larger image


Fig. 6
Effect of stabilized rice bran on serum (A) and liver (B) triglyceride concentration in C57BL/6 mice. Values are expressed as mean ± SE. abDifferent letters over the bars indicate that the mean values are significantly different (p < 0.05) by Duncan's multiple range test. ND: normal diet, HSD: high sucrose diet, HSD-SRB: high sucrose diet in which 20% dietary calorie nutrient (g) is replaced with stabilized rice bran.
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Tables


Table 1
Composition of experimental diets
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Table 2
Effect of stabilized rice bran on body weight gain, food intake and liver weight in C57BL/6 mice
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Table 3
Effect of stabilized rice bran on adipose tissue weight in C57BL/6 mice
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Table 4
Effect of stabilized rice bran on fasting blood glucose in C57BL/6 mice
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Notes

This work was supported by 2012 Technology Commercialization Support Program from Ministry of Agriculture, Food and Rural Affairs.

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