Journal List > J Nutr Health > v.46(4) > 1081300

J Nutr Health. 2013 Aug;46(4):324-331. Korean.
Published online August 31, 2013.  https://doi.org/10.4163/jnh.2013.46.4.324
© 2013 The Korean Nutrition Society
Effect of green tea intake on blood lipids, platelet aggregation, antioxidant and liver parameters in Jeju volunteer diving woman
Mi-Sook Kim,1 Min-Sook Kang,2 Sung-Hee Ryou,1 Young-In Moon,3 and Jung-Sook Kang1
1Department Foods & Nutrition, Jeju National University, Jeju 690-756, Korea.
2Nutrition Service Team, Jeju National University Hospital, Jeju 690-767, Korea.
3Jeju Agricultural Development and Technology Extention Center, Jeju 695-971, Korea.

To whom correspondence should be addressed. (Email: jungkang@jejunu.ac.kr )
Received June 20, 2013; Revised July 09, 2013; Accepted August 06, 2013.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

We investigated dietary effects of green tea powder (GTP) on plasma lipids, platelet aggregation, hemolysis, plasma TBARS, and liver enzymes. Thirty one volunteer diving women living on Jeju island consumed 4 g green tea powder daily for a period of four weeks and data for the study subjects were analyzed on the basis of diagnostic criteria for blood pressure (BP)(≥ 140/90 mmHg), plasma total cholesterol (TC)(≥ 200 mg/dL), and triglyceride (TG)(≥ 150mg/dL). Subjects with high BP had significantly higher TC and TG than those with normal BP. Subjects with higher TC had higher TG, and those with higher TG had lower HDL cholesterol. Platelet aggregation in the initial slope was significantly higher in subjects with normal BP, normal TC, or normal TG than their counterparts in high BP, TC, and TG. HDL cholesterol after GTP intake increased only in subject groups with normal BP, normal TC, or normal TG, and plasma TG after GTP intake decreased only in groups with higher BP, higher TG, or higher TC. Plasma TC and TG in subjects with normal BP increased after GTP intake. GTP intake caused a decrease in the initial slope of platelet aggregation in all subject groups with little effect on maximum aggregation. Total bilirubin showed a significant increase and GOT increased in all subject groups after GTP intake. Beneficial effects of short term intake of green tea powder might differ depending on the subject conditions in terms of blood pressure, plasma lipids, and other cardiovascular conditions. However, with the hypolipidemic, antithrombotic, and antioxidant actions of its bioactive flavonoids, long term usage of GTP or brewed green tea may provide preventive effects against cardiovascular disease.

Keywords: green tea powder; hypolipidemic; antithrombotic; antioxidant; women

Figures


Fig. 1
Comparison of plasma lipids before and after green tea powder intake based on diagnostic criteria for blood pressure (BP), total-cholesterol (T-chol), and triglyceride (TG).

*: p < 0.05.

Click for larger image


Fig. 2
Comparison of hematocrit and platelet aggregation before and after green tea powder intake based on diagnostic criteria for blood pressure (BP), total-cholesterol (T-chol), and triglyceride (TG).

*: p < 0.05.

Click for larger image


Fig. 3
Comparison of hemolysis and plasma TBARS before and after green tea powder intake based on diagnostic criteria for blood pressure (BP), total-choleserol (T-chol), and triglyceride (TG).

*: p < 0.05.

Click for larger image


Fig. 4
Comparison of plasma GOT, GPT, alkaline phosphatase, and T-bilirubin before and after green tea powder intake based on diagnostic criteria for blood pressure (BP), total-cholesterol (T-chol), and triglyceride (TG).

*: p < 0.05, **: p < 0.01.

Click for larger image

Tables


Table 1
Comparison of clinical data based on diagnostic criteria for blood pressure, plasma T-cholesterol and triglyceride before green tea powder intake
Click for larger image


Table 2
Plasma lipids, hematologic parameters, platelet aggregation and pathological indices of the study subjects before and after green tea powder intake
Click for larger image

Notes

This is a partial work supported by the Korea Research Grant (R05-2001-000-00718-0).

References
1. Shin MK. Green tea science. Korean J Diet Cult 1994;9(4):433–445.
2. Trevisanato SI, Kim YI. Tea and health. Nutr Rev 2000;58(1):1–10.
3. Fuhrman BJ, Pfeiffer RM, Wu AH, Xu X, Keefer LK, Veenstra TD, Ziegler RG. Green tea intake is associated with urinary estrogen profiles in Japanese-American women. Nutr J 2013;12:25.
4. Shen CL, Smith BJ, Lo DF, Chyu MC, Dunn DM, Chen CH, Kwun IS. Dietary polyphenols and mechanisms of osteoarthritis. J Nutr Biochem 2012;23(11):1367–1377.
5. Sabu MC, Smitha K, Kuttan R. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J Ethnopharmacol 2002;83(1-2):109–116.
6. Westerterp-Plantenga MS, Lejeune MP, Kovacs EM. Body weight loss and weight maintenance in relation to habitual caffeine intake and green tea supplementation. Obes Res 2005;13(7):1195–1204.
7. Kono S, Shinchi K, Wakabayashi K, Honjo S, Todoroki I, Sakurai Y, Imanishi K, Nishikawa H, Ogawa S, Katsurada M. Relation of green tea consumption to serum lipids and lipoproteins in Japanese men. J Epidemiol 1996;6(3):128–133.
8. Kuriyama S. The relation between green tea consumption and cardiovascular disease as evidenced by epidemiological studies. J Nutr 2008;138(8):1548S–1553S.
9. Elisaf M, Karabina SA, Bairaktari E, Goudevenos JA, Siamopoulos KC, Tselepis AD. Increased platelet reactivity to the aggregatory effect of platelet activating factor, in vitro, in patients with heterozygous familial hypercholesterolaemia. Platelets 1999;10(2-3):124–131.
10. Sugatani J, Fukazawa N, Ujihara K, Yoshinari K, Abe I, Noguchi H, Miwa M. Tea polyphenols inhibit acetyl-CoA:1-alkyl-snglycero-3-phosphocholine acetyltransferase (a key enzyme in platelet-activating factor biosynthesis) and platelet-activating factor-induced platelet aggregation. Int Arch Allergy Immunol 2004;134(1):17–28.
11. Deana R, Turetta L, Donella-Deana A, Donà M, Brunati AM, De Michiel L, Garbisa S. Green tea epigallocatechin-3-gallate inhibits platelet signalling pathways triggered by both proteolytic and non-proteolytic agonists. Thromb Haemost 2003;89(5):866–874.
12. Saffari Y, Sadrzadeh SM. Green tea metabolite EGCG protects membranes against oxidative damage in vitro. Life Sci 2004;74(12):1513–1518.
13. Biswas S, Bhattacharyya J, Dutta AG. Oxidant induced injury of erythrocyte-role of green tea leaf and ascorbic acid. Mol Cell Biochem 2005;276(1-2):205–210.
14. Hirano-Ohmori R, Takahashi R, Momiyama Y, Taniguchi H, Yonemura A, Tamai S, Umegaki K, Nakamura H, Kondo K, Ohsuzu F. Green tea consumption and serum malondialdehyde-modified LDL concentrations in healthy subjects. J Am Coll Nutr 2005;24(5):342–346.
15. Hsu SP, Wu MS, Yang CC, Huang KC, Liou SY, Hsu SM, Chien CT. Chronic green tea extract supplementation reduces hemodialysis-enhanced production of hydrogen peroxide and hypochlorous acid, atherosclerotic factors, and proinflammatory cytokines. Am J Clin Nutr 2007;86(5):1539–1547.
16. Sakong J. Diving patterns and diving related disease of diving fishermen in Korea. Korean J Prev Med 1998;31(1):139–156.
17. Draper HH, Csallany AS. A simplified hemolysis test for vitamin E deficiency. J Nutr 1969;98(4):390–394.
18. Yagi K. A simple fluorometric assay for lipoperoxide in blood plasma. Biochem Med 1976;15(2):212–216.
19. Karthikeyan G, Teo KK, Islam S, McQueen MJ, Pais P, Wang X, Sato H, Lang CC, Sitthi-Amorn C, Pandey MR, Kazmi K, Sanderson JE, Yusuf S. Lipid profile, plasma apolipoproteins, and risk of a first myocardial infarction among Asians: an analysis from the INTERHEART Study. J Am Coll Cardiol 2009;53(3):244–253.
20. de Freitas EV, Brandão AA, Pozzan R, Magalhães ME, Fonseca F, Pizzi O, Campana E, Brandão AP. Importance of high-density lipoprotein-cholesterol (HDL-C) levels to the incidence of cardiovascular disease (CVD) in the elderly. Arch Gerontol Geriatr 2011;52(2):217–222.
21. Kang JA, Chae IS, Song YB, Kang JS. Effects of green tea on weight gain, plasma and liver lipids and lipid peroxidation in pair fed rats. Korean J Nutr 2008;41(7):602–611.
22. Bursill CA, Abbey M, Roach PD. A green tea extract lowers plasma cholesterol by inhibiting cholesterol synthesis and upregulating the LDL receptor in the cholesterol-fed rabbit. Atherosclerosis 2007;193(1):86–93.
23. Raederstorff DG, Schlachter MF, Elste V, Weber P. Effect of EGCG on lipid absorption and plasma lipid levels in rats. J Nutr Biochem 2003;14(6):326–332.
24. Han SG, Kang MS, Ryou SH, Hwang SW, Kang JS. Effect of prickly pear cactus (Opuntina ficus-indica) intake on blood lipids, platelet aggregation, antioxidant and liver parameters in volunteer diving woman. Korean J Nutr 2012;45(5):462–469.
25. von Lewinski F, Riggert J, Paulus W. Towards a rationale of platelet aggregation monitoring in stroke prophylaxis? J Stroke Cerebrovasc Dis 2009;18(2):111–115.
26. Lukasik M, Dworacki G, Michalak S, Kufel-Grabowska J, Golanski J, Watala C, Kozubski W. Aspirin treatment influences platelet-related inflammatory biomarkers in healthy individuals but not in acute stroke patients. Thromb Res 2011;128(5):e73–e80.
27. Chello M, Spadaccio C, Patti G, Lusini M, Barbato R, Goffredo C, Di Sciascio G, Covino E. Simvastatin reduces platelet-endocardium adhesion in atrial fibrillation. Atherosclerosis 2008;197(2):588–595.
28. Lee YJ, Chung HY, Kwak HK, Yoon S. The effects of A. senticosus supplementation on serum lipid profiles, biomarkers of oxidative stress, and lymphocyte DNA damage in postmenopausal women. Biochem Biophys Res Commun 2008;375(1):44–48.