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Abstract
Purpose
We wanted to investigate the usefulness of Mycofast (MYCOFAST® Evolution 2, International Microbio, France) for Ureaplasma urealyticum (U. urealyticum) and Mycoplasma hominis (M. hominis) in association with nongonococcal genitourinary infections.
Materials and Methods
530 patients visited our department for genitourinary infection symptoms or for the evaluation of sexually transmitted disease. The genital swabs were first vortexed in Mycofast transport broth. A volume of 100µl of liquid sample was innoculated to each well of the Mycofast broths and 0.5µl of liquid sample was innoculated to A7 agar culture media (International Microbio, France). The Mycofast broths were incubated at 35-37℃ for 36 hrs, and the A7 agar culture media was incubated for 4 days. We compaired Mycofast with A7 agar culture for the sensitivity, specificity, the positive and negative predictive values and the antibiotic susceptable profiles.
Results
Of the 530 samples submitted, 165 samples were positive by the A7 agar culture and 162 samples were positive by Mycofast. 157 samples were positive by both methods. Of the 365 samples that were negative by the A7 agar culture, 360 samples were also negative by the Mycofast. In this study, Mycofast had a sensitivity and specificity of 95% and 98%, respectively, and a positive and negative predictive value of 96% and 97%, respectively. The Mycofast drug susceptibility tests indicate a high susceptibility to doxycyclin as follows: U. urealyticum: 86.3%; M. hominis: 85.0% and both organisms with simultaneous isolation: 75.8%.
Conclusions
Mycofast was an easy test to perform and it proved to be a practical and reliable method for isolating the Mycoplasma and Ureaplasma species for making the diagnosis of nongonococcal genitourinary infections, and it showed the added benefit of determining the limited susceptibilities of the isolated strains.
Figures and Tables
Fig. 1
Detection of Ureaplasma urealyticum and Ureaplasma urealyticum at 104 CCUs (color change units) mycofast/ml, as diagnosed by the color change of the enumeration and identification well on the MYCOFAST® Evolution 2 tray.
Fig. 3
(A) The microscopic finding of Ureaplasma urealyticum in the A7 agar culture medium. The colonies show a brown or black urchin-like appearance. (B) The microscopic finding of Mycoplasma hominis in the A7 agar culture medium. The colonies have a characteristic fried egg appearance. (C) The microscopic finding of Ureaplasma urealyticum and Mycoplasma hominis as cultured simultaneously in A7 agar culture medium.
Table 1
Isolation of U. urealyticum, M. hominis according to age and gender in the group that was simultaneously positive on the MYCOFAST® Evolution 2 and A7 agar cultures
Table 2
Comparison of U. urealyticum and M. hominis by MYCOFAST® Evolution 2 with the A7 agar culture results
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