Journal List > Korean J Nutr > v.46(2) > 1043971

Korean J Nutr. 2013 Apr;46(2):119-125. Korean.
Published online April 30, 2013.  https://doi.org/10.4163/kjn.2013.46.2.119
© 2013 The Korean Nutrition Society
Effects of medicinal herb water extracts on expression of hepatic glucokinase, pyruvate dehydrogenase and acetyl-CoA carboxylase mRNA
Hyun Sook Kim,1 Tae Woo Kim,1,2 Dae Jung Kim,1,2 Jae Sung Lee,2 and Myeon Choe1,2
1Well-being Bioproducts RIC Center, Kangwon National University, Chuncheon 200-701, Korea.
2Department of Bio-Health Technology, Kangwon National University, Chuncheon 200-701, Korea.

To whom correspondence should be addressed. (Email: mchoe@kangwon.ac.kr )
Received January 21, 2013; Revised February 06, 2013; Accepted February 28, 2013.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

We studied the anti-diabetic effects of medicinal herb water extracts on expression of hepatic glucokinase (GCK), pyruvate dehydrogenase (PDH), and acetyl-CoA carboxylase (ACC) mRNA. The medicinal herbs used for experiments were Cornus officinalis (CO), Paeonia suffruticosa Andrews (PSA), Discorea japonica Thunb. (DJ), Rehmannia glutinosa (RG), Lycium chinense (LC), and Pyrus pyrifolia (PP). For GCK mRNA expression, CO, RG, and LC water extracts exhibited a more effective activity than other extracts. Cells treated with RG and LC water extracts showed an increase in expression of PDH mRNA to 191% and 124%, respectively, compared to control. Expression of ACC mRNA was significantly higher in LC water extract. These data indicate that CO, RG, and LC water extracts stimulates expression of hepatic GCK, PDH, and ACC mRNA.

Keywords: anti-diabetic; glucokinase; pyruvate dehydrogenase; acetyl-CoA carboxylase; medicinal herb

Figures


Fig. 1
Effect of medicinal herb water extracts on the cell viability in HepG2 cells. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.
Click for larger image


Fig. 2
Glucokinase mRNA expression of the water extracts from medicinal herbs. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.
Click for larger image


Fig. 3
Pyruvate dehydrogenase mRNA expression of the water extracts from medicinal herbs. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.
Click for larger image


Fig. 4
Acetyl-CoA carboxylase mRNA expression of the water extracts from medicinal herbs. Results are from three experiments and expressed as Mean ± SE (n = 3). Means with the different letters are significantly different by Duncan's multiple range test (p < 0.05). CO: Cornus officinalis, PSA: Paeonia suffruticosa Andrews, DJ: Discorea japonica Thunb, RG: Rehmannia glutinosa, LC: Lycium chinense, PP: Pyrus pyrifolia.
Click for larger image

Tables


Table 1
PCR primer sequences
Click for larger image

Notes

This work was supported by grants of the Institute of Bioscience Biotechnology, Well-being Bioproducts Regional Innovation Center, and LINC Project Group in Kangwon National University.

References
1. Egede LE, Ellis C. Diabetes and depression: global perspectives. Diabetes Res Clin Pract 2010;87(3):302–312.
2. Joo CN, Koo JH, Lee HB. Study on the hypoglycemic action of the fat soluble fraction of Panax ginseng C.A. meyer in streptozotocin induced diabetic rats. Korean J Ginseng Sci 1993;17(1):13–21.
3. Joo CN, Kim SJ. Hypoglycemic action of the fat soluble fraction of Panax ginseng C.A. meyer in streptozotocin induced diabetic rats. Korean J Ginseng Sci 1993;17(2):101–108.
4. Kim OK. Antidiabetic and antioxidative effect of Lycii fructus in streptozotocin-induced diabetic rats. Korean J Pharmacogn 2009;40(2):128–136.
5. Park MJ, Kang SJ, Kim AJ. Hypoglycemic effect of Angelica gigas Naki extract in streptozotocin-induced diabetic rats. Korean J Food Nutr 2009;22(2):246–251.
6. Takeda Y, Inoue H, Honjo K, Tanioka H, Daikuhara Y. Dietary response of various key enzymes related to glucose metabolism in normal and diabetic rat liver. Biochim Biophys Acta 1967;136(2):214–222.
7. Huang TH, Yang Q, Harada M, Uberai J, Radford J, Li GQ, Yamahara J, Roufogalis BD, Li Y. Salacia oblonga root improves cardiac lipid metabolism in Zucker diabetic fatty rats: modulation of cardiac PPAR-α-mediated transcription of fatty acid metabolic genes. Toxicol Appl Pharmacol 2006;210(1-2):78–85.
8. Kang SY, Paeng JR, Seo KS, Woo JT, Kim SW, Yang IM, Kim JW, Kim YS, Kim KW, Choi YK. Regulation of glucokinase gene expression and activity in the liver of diabetic rats. Korean J Med 1994;47(2):203–209.
9. Lee EB, Choi BC, Cho TS. Pharmacological studies on ether fraction of Corni fructus. Yakhak Hoeji 1985;29(1):1–10.
10. Kim OK. Antidiabetic and antioxidative effects of Corni fructus in streptozotocin-induced diabetic rats. J Korean Oil Chem Soc 2005;22(2):157–167.
11. Joo HK, Jang DJ. Effects of Shanshuyu (Cornus officinalis Sieb) tea and market teas feeding on the hematology and liver function of rat. Korean J Diet Cult 1989;4(3):257–264.
12. Seo KI, Lee SW, Yang KH. Antimicrobial and antioxidative activities of Corni fructus extracts. Korean J Postharvest Sci Technol 1999;6(1):99–103.
13. Fukuhara Y, Yoshida D. Paeonol: a bio-antimutagen isolated from a crude drug, Moutan cortex. Agric Biol Chem 1987;51(5):1441–1442.
14. Mitsuo M, Maruyama H, Kameoka H. Essential oil constituents of "Moutan radicis cortex" Paeonia Moutan Sims. (P. suffruticosa Andrews). Agric Biol Chem 1983;47(12):2925–2927.
15. You JK, Chung MJ, Kim DJ, Seo DJ, Park JH, Kim TW, Choe M. Antioxidant and tyrosinase inhibitory effects of Paeonia suffruticosa water extract. J Korean Soc Food Sci Nutr 2009;38(3):292–296.
16. Park S, Jun DW, Park CH, Jang JS, Park SK, Ko BS, Kim BJ, Choi SB. Hypoglycemic effects of crude extracts of Moutan radicis cortex. Korean J Food Sci Technol 2004;36(3):472–477.
17. Lee ST, Chae YH. In: Botany of herbal resource. Seoul: Hakmun Publishing Co.; 1996. pp. 130.
18. Jeong HJ, Kim IH. Comparative studies on the antidiabetic activities of Rehmanniae radices -the effect of Rehmanniae radices extracts on streptozotocin-indeced hyperglycemia in rats-. Chung-Ang J Pharm Sci 1990;4:22–31.
19. Cho YJ. Charactrization of biological activities of Rehmannia glutinosa extracts. J Life Sci 2012;22(7):943–949.
20. Cho SI. Effects of the Rehmanniae radix preparat on ovariectomized rats. Korean J Herbol 2005;20(4):61–67.
21. Sheo HJ, Jun SJ, Lee MY. Effects of Lycii fructus extract on experimentally induced liver damage and alloxan diabetes in rabbits. J Korean Soc Food Nutr 1986;15(2):136–143.
22. Kim BW, Roh KS. Study on the activity of GOT and GPT in the hepatotoxic rat treated Lycium chinense mill. Korean J Biomed Lab Sci 2000;6(3):187–192.
23. Yoon CG, Kim HH, Chae SN, Oh MJ, Lee GH. Hepatic oxygen free radical and alcohol metabolizing enzyme activities in rats fed diets supplemented with Lycium chinense ethanol extract. J Korean Soc Food Sci Nutr 2001;30(4):668–672.
24. Ahn BY, Gwak JS, Ryu SH, Moon GS, Choi DS, Park SH, Han JH. Protective effect of water extract of Lycii cordex radicis on lipid peroxidation of rat skin exposed to ultraviolet B radiation. Agric Chem Biotechnol 2002;45(4):218–222.
25. Yu TJ. In: The food guide. Seoul: Munundang; 1989. pp. 166.
26. Choi HJ, Park JH, Han HS, Son JH, Son GM, Bae JH, Choi C. Effect of polyphenol compound from Korean pear (Pyrus pyrifolia Nakai) on lipid metabolism. J Korean Soc Food Sci Nutr 2004;33(2):299–304.
27. An BJ, Lee JT, Kwak JH, Park JM, Lee JY, Son JH, Bae JH, Choi C. Biological activity of polyphenol group fraction from Korean pear peel. J Korean Soc Appl Biol Chem 2004;47(1):92–95.
28. Chung MJ, Walker PA, Brown RW, Hogstrand C. ZINC-mediated gene expression offers protection against H2O2-induced cytotoxicity. Toxicol Appl Pharmacol 2005;205(3):225–236.
29. Nakamaru K, Matsumoto K, Taguchi T, Suefuji M, Murata Y, Igata M, Kawashima J, Kondo T, Motoshima H, Tsuruzoe K, Miyamura N, Toyonaga T, Araki E. AICAR, an activator of AMP-activated protein kinase, down-regulates the insulin receptor expression in HepG2 cells. Biochem Biophys Res Commun 2005;328(2):449–454.
30. Choi HJ, Kim SH, Oh HT, Chung MJ, Cui CB, Ham SS. Effects of Adenophora triphylla ethylacetate extract on mRNA levels of antioxidant enzymes in human HepG2 cells. J Korean Soc Food Sci Nutr 2008;37(10):1238–1243.
31. Ferre T, Pujol A, Riu E, Bosch F, Valera A. Correction of diabetic alterations by glucokinase. Proc Natl Acad Sci U S A 1996;93(14):7225–7230.
32. Muñoz MC, Barberà A, Domínguez J, Fernandez-Alvarez J, Gomis R, Guinovart JJ. Effects of tungstate, a new potential oral antidiabetic agent, in Zucker diabetic fatty rats. Diabetes 2001;50(1):131–138.
33. Postic C, Shiota M, Niswender KD, Jetton TL, Chen Y, Moates JM, Shelton KD, Lindner J, Cherrington AD, Magnuson MA. Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic β cell-specific gene knock-outs using Cre recombinase. J Biol Chem 1999;274(1):305–315.
34. Pari L, Rajarajeswari N. Efficacy of coumarin on hepatic key enzymes of glucose metabolism in chemical induced type 2 diabetic rats. Chem Biol Interact 2009;181(3):292–296.
35. Jung UJ, Lee MK, Park YB, Kang MA, Choi MS. Effect of citrus flavonoids on lipid metabolism and glucose-regulating enzyme mRNA levels in type-2 diabetic mice. Int J Biochem Cell Biol 2006;38(7):1134–1145.
36. Kondeti VK, Badri KR, Maddirala DR, Thur SK, Fatima SS, Kasetti RB, Rao CA. Effect of Pterocarpus santalinus bark, on blood glucose, serum lipids, plasma insulin and hepatic carbohydrate metabolic enzymes in streptozotocin-induced diabetic rats. Food Chem Toxicol 2010;48(5):1281–1287.
37. Ko BS, Kwon DY, Hong SM, Park S. In vitro anti-diabetic effects of crude extracts of Platycodi radix. Korean J Food Sci Technol 2007;39(6):701–707.
38. Shimizu T, Parker JC, Najafi H, Matschinsky FM. Control of glucose metabolism in pancreatic β-cells by glucokinase, hexokinase, and phosphofructokinase. Model study with cell lines derived from β-cells. Diabetes 1988;37(11):1524–1530.
39. Matschinsky FM. Glucokinase as glucose sensor and metabolic signal generator in pancreatic β-cells and hepatocytes. Diabetes 1990;39(6):647–652.
40. Lee HA, Sim HS, Choi KJ, Lee HB. Hypoglycemic action of red ginseng components (II): investigation of the effect of fat soluble fraction from red ginseng on enzymes related to glucose metabolism in cultured rat hapatocytes. Korean J Ginseng Sci 1998;22(1):51–59.
41. Kim HS, Ro YJ, Choe M. Effects of Cordyceps militaris on key enzymes of carbohydrate metabolism. J Korean Soc Food Sci Nutr 2005;34(10):1531–1535.
42. Choe M, Kim DJ, Lee HJ, You JK, Seo DJ, Lee JH, Chung MJ. A study on the glucose-regulating enzymes and antioxidant activities of water extracts from medicinal herbs. J Korean Soc Food Sci Nutr 2008;37(5):542–547.
43. Thampy GK, Haas MJ, Mooradian AD. Troglitazone stimulates acetyl-CoA carboxylase activity through a post-translational mechanism. Life Sci 2000;68(6):699–708.
44. Kim DJ, Chung MJ, You JK, Seo DJ, Kim JM, Choe M. Effect of medicinal plant water extracts on glucose-regulating enzyme activities in Goto-Kakizaki rat liver cytosol. J Korean Soc Food Sci Nutr 2009;38(10):1331–1335.