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Abstract
This study was aimed to investigate whether soy protein hydrolysates had beneficial effects on serum and tissue lipid contents and appetite-related hormones as compared with intact soy protein. Four-week-old male Sprague-Dawley rats were fed AIN-93M diet containing high fat (18% w/w) with low protein (10% w/w). After four weeks, the rats were divided into four groups (n = 8/group) and fed experimental diets with different nitrogen sources and levels, respectively; 10% soy protein isolate (10SPI), 25% soy protein isolate (25SPI), 25% soy protein hydrolysates (25SPH) and 25% soy macro-peptide fractions (25SPP, MW ≥ 10,000) for six weeks. Weight gain was significantly higher in 25% nitrogen sources-fed groups than in 10% group (10SPI). In 25SPP, perirenal fat mass and serum total lipid were significantly lower than in other groups. As for appetite-related hormones, serum ghrelin concentration was not shown to be different among groups but leptin concentration was significantly decreased in 25SPP. It can be concluded that soy macro-peptide fractions as compared with intact soy protein may have beneficial effects on reducing fat mass and serum lipid.
Figures and Tables
Fig. 1
The concentration of serum glucose and insulin in rats fed high fat diets containing various nitrogen sources. Groups are the same as in Table 1. Values are means ± SE of 8 rats per group. Bars with different superscripts are significantly different at p < 0.05 by Duncan's multiple range test.
Fig. 2
The concentration of serum ghrelin and leptin in rats fed high fat diets containing various nitrogen sources. Groups are the same as in Table 1. Values are means ± SE of 8 rats per group. Bars with different superscripts are significantly different at p < 0.05 by Duncan's multiple range test.
Table 1
Composition of experimental diets (g/kg diet)1)
1) Experimental diets were prepared according to AIN-93M Purified Diets
2) 10SPI: 18% beef tallow + 10% soy protein isolate, 25SPI: 18% beef tallow + 25% soy protein isolate, 25SPH: 18% beef tallow + 25% soy protein hydrolysate, 25SPPP: 18% beef tallow + 25% soy macro-peptide fraction
3) Values are adjusted from considering of protein content contained in SPI, SPH and SPP
4) AIN-93M Mineral mix
5) AIN-93 Vitaminl mix
Table 2
Body weight, weight gain and food intake of the rats fed high fat containing various nitrogen sources
Table 3
Fresh weights of liver, kidney, testis and spleen in rats fed high fat diets containing various nitrogen sources (g/100 g BW)
Table 4
Fresh weight of perirenal and epididymal fat in rats fed high fat containing various nitrogen sources
Table 5
The concentration of serum total lipid, triglyceride, total cholesterol and HDL cholesterol in rats fed high fat containing various nitrogen sources (mg/dL)
Table 6
The contents of liver total lipid, triglyceride and total cholesterol in rats fed high fat containing various nitrogen sources (mg/g)
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