The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract

Seulgi Hong; Dongmyong Jeong; Kiyoung Kim; Eunhee Hwang

doi:10.4163/kjn.2010.43.2.105

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Hong, Jeong, Kim, and Hwang: The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract

Original Article

Korean Journal of Nutrition

Korean Journal of Nutrition 2010; 43(2): 105-113.

Published online: 29 April 2010

DOI: https://doi.org/10.4163/kjn.2010.43.2.105

The Composition of the Root of Ixeris dentata var. albiflora Nakai. and Cell Viability and DPPH Radical Scavenging Activities of its Extract

Seulgi Hong¹, Dongmyong Jeong², Kiyoung Kim³, Eunhee Hwang⁴

¹Jangheungun Mushroom Research Institute, Jeonnam 529-851, Korea.

²Institute of Biomedical Engeneering Research, Wonkwang University, Iksan 570-711, Korea.

³Institute for Better Living, Wonkwang University, Iksan 570-711, Korea.

⁴Department of Food & Nutrition, Institute for Better Living, Wonkwang University, Iksan 570-711, Korea.

To whom correspondence should be addressed. (ehhwang@wku.ac.kr)

Received 7 December 2009 Revised 6 January 2010 Accepted 17 February 2010

Abstract

Ixeris dentata var. albiflora Nakai, a herbal plant, is often used to make a strong stomach as an antiphlogistic used when dyspepsia and to improve appetite in Korea and China. And also it is used for adult diseases such as diabetes and liver diseases as Korean traditional medicine. In this study, the composition and DPPH radical scavenging activities of the root of Ixeris dentata var. albiflora Nakai and its effects on cell viability on vero and chang cells were investigated. Moisture, crude ash, crude protein and crude lipid were 79.14, 2.49, 8.28 and 2.56 g/100 g respectively. The highest mineral content was K. The major free sugars were glucose, fructose and sucrose. Major fatty acid are linoleic acid, palmic acid and linolenic acid. Major amino acids were glutamic acid, arginine and aspartic acid and the total contents of amino acids were 28.12 mg/g. The methanol extracts were further fractionated with n-hexane, chloroform, ethylacetate, butanol and water to get an active fraction. In addition, cell viabilities in each fraction were determined. Methanol extract, butanol, and aqueous fraction showed strong survival rates in vero cell and chang cell viability test, and hexane, chloroform, and ethylacetate fraction were examined for toxin in a cell. The root of Ixeris dentata var. albiflora Nakai had scavenging activities against DPPH radicals in a dose-dependent assay. Ethylacetate fraction's SC50 was 6.8 µg/mL, very strong DPPH radical scavenging activities, but water fraction did not show any activity.

Keywords: root of Ixeris dentata var. albiflora Nakai, composition, cell viability, DPPH radical scavenging activities

Figures and Tables

Fig. 1

Extraction and fraction of the root of Ixeris dentata var. albiflora Nakai.

Table 1

Contents of moisture, crude ash, crude protein and crude lipid in the root of Ixeris dentata var. albiflora Nakai

Data show mean ± S.E. in triplicate. 1) freeze dried material

Table 2

Contents of minerals in the root of Ixeris dentata var.albiflora Nakai

Table 3

Contents of free sugars and total phenolics compounds in the root of Ixeris dentata var. albiflora Nakai

Table 4

Contents of fatty acid in the root of Ixeris dentata var. albiflora Nakai

Table 5

Contents of amino acid in the root of Ixeris dentata var. albiflora Nakai

1) essential amino acids

Table 6

Cell viability of methanol extract and systematic fraction from the root of Ixeris dentata var. albiflora Nakai on vero cell

1) I.D.: Ixeris dentata var. albiflora Nakai, 2) ME: Methanol extract, 3) HE: Hexane fraction, 4) CH: Chloroform fraction

5) EA: Ethylacetate fraction, 6) BuOH: Butanol fraction

7) WE: Water extract. Data show mean ± S.E. in triplicate, Cells were pretreated with various diluted concentrations of I.D. for 1 day and cell viability was determined by the MTT assay.

^*: p < 0.05, ^**: p < 0.005, sign-ificantly different from the control

Table 7

Cell viability of methanol extract and systematic fraction from the root of Ixeris dentata var. albiflora Nakai on chang cell

1) I.D.: Ixeris dentata var. albiflora Nakai, 2) ME: Methanol extract, 3) HE: Hexane fraction, 4) CH: Chloroform fraction

5) EA: Ethylacetate fraction, 6) BuOH: Butanol fraction

7) WE: Water extract. Data show mean ± S.E. in triplicate, Cells were pretreated with various diluted concentrations of I.D. for 2 day and cell viability was determined by the MTT assay.

^*: p < 0.05, ^**: p < 0.005, significantly different from the control

Table 8

Scavenging activity of methanol extract and systematic fraction from the root of Ixeris dentata var. albiflora Nakai against DPPH

1) I.D.: Ixeris dentata var. albiflora Nakai, 2) ME: Methanol extract, 3) HE: Hexane fraction, 4) CH: Chloroform fraction

5) EA: Ethylacetate fraction, 6) BuOH: Butanol fraction, 7) WE: Water extract. Data show mean ± S.E. in triplicate

Table 9

SC50 value of various fractions in the root of Ixeris dentata var. albiflora Nakai

ME: Methanol extract, HE: Hexane fraction, CH: Chloroform fraction, EA: Ethylacetate fraction, BuOH: Butanol fraction, WE: Water extract, BHT: buthyl hydroxy toluene

Notes

This research was supported by 2008 grant from Wonkwang University.

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