Journal List > Korean J Nutr > v.42(3) > 1043752

Ryu, Kim, and Kim: Effects of Plant Water Extract Codonopsis Lanceolatae on Mouse Immune Cell Activation Ex Vivo

Abstract

Codonopsis lanceolatae has been used as one of the traditional remedies as well as food source. However, few studies on their immunomodulating effects have been reported. We previously reported that ex vivo supplementation of Codonopsis lanceolatae water extracts enhanced splenocyte proliferation compared to the control group. In order to elucidate its ex vivo effect, six to seven week old balb/c mice were fed ad libitum on a chow diet and water extracts of Codonopsis lanceolatae were orally administrated every other day for four weeks at two different concentrations (50 and 500 mg/kg B.W.). After preparing the single cell suspension, the proliferation of splenocytes was determined by MTT (3-[4,5-dimethylthiazol-2-y]-2,5-diphenyl terazolium bromide) assay. The production of cytokine (IL-1β, IL-6, TNF-α), secreted by macrophages stimulated with LPS or not, was detected by ELISA assay using a cytokine kit. After 48 hrs of incubation with the mitogen (ConA or LPS) stimulation, the mice splenocyte proliferation in experimental group was statistically increased at two different concentrations than that in control group. The cytokines production was more significantly enhanced at the lower supplementation (500 mg/kg B.W.) group rather than higher concentration (500 mg/kg B.W.) compared to the control group. The results of this study may suggest that the supplementation of water extract of plant mixture could regulate the immune function by increasing the splenocyte proliferation and enhance the immune function through regulating cytokine production capacity by activated macrophages in mice.

Figures and Tables

Fig. 1
Flow diagram for water extraction procedure.
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Fig. 2
Study design of ex vivo experiment.
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Table 1
Proliferation index of splenocytes of mice orally adminisered with water extracts of Codonopsis lanceolatae for 4 weeks
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1) Proliferation index = mean of O.D. in test wells/mean of O.D. in control wells

2) Means with different letters (a, b) are significantly different from each other at α= 0.05 as determined by Duncan's multiple range test (a > b)

Table 2
IL-1β production by activated peritoneal macrophages of mice orally administered with water extracts of Codonopsis lanceolatae for 4 weeks
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1) Macrophages were incubated with or without (control) mixture water extracts for 48 h

2) The data present the mean values ± S.D. n = 4 The different letters (a, b, c) within every mitogen groups are significantly different from each other at α = 0.05 as determined by Duncan's multiple range test (a > b > c)

3) The cytokine concentrations were determined by triplicates cultured supernatant cells and values are mean ± S.D.

Table 3
IL-6 production by activated peritoneal macrophages of mice orally administered with water extracts of Codonopsis lanceolatae for 4 weeks
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1) Macrophages were incubated with or without (control) mixture water extracts for 48 h

2) The data present the mean values ± S.D. n = 4 The different letters (a, b, c) within every mitogen groups are significantly different from each other at α = 0.05 as determined by Duncan's multiple range test (a > b > c)

3) The cytokine concentrations were determined by triplicates cultured supernatant cells and values are mean ± S.D.

Table 4
TNF-α production by activated peritoneal macrophages of mice orally administered with water extracts of Codonopsis lanceolatae for 4 weeks
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1) Macrophages were incubated with or without (control) mixture water extracts for 48 h

2) The data present the mean values ± S.D. n = 4 The different letters (a, b, c) within every mitogen groups are significantly different from each other at α = 0.05 as determined by Duncan's multiple range test (a > b > c)

3) The cytokine concentrations were determined by triplicates cultured supernatant cells and values are mean ± S.D.

Notes

This work was supported by Korea Food Research Institute, 2008.

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