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Abstract
The study aim was to investigate the antihypertensive effect after oral supplementation of dried radish leaves powder (DRLP). Angiotensin-converting enzyme (ACE) activity was measured by spectrophotometric assay. The systolic blood pressure (SBP) was measured in spontaneously hypertensive (SHR) and normotensive rats (Wistar) by the tail cuff method after a 4-week diet with DRLP at the level of 2.5% or 5%. The supplementation of DRLP decreased SBP of SHR although the 5% supplementation level did not show any more pronounced effect than the 2.5% level did. The decrease in the SBP observed for both 2.5% and 5% DRLP was accompanied by significant increases of the urinary Na and K excretion. The DRLP supplementation showed a potent ACE-inhibitory activity in pulmonary tissue from both hypertensive and normotensive rats. However, the DRLP supplementation did not affect the SBP in normotensive rats. These results indicated that DRLP exerted an antihypertensive effect in SHR due to the decreased ACE activity and increased urinary Na excretion.
Figures and Tables
Table 1
Composition of experimental diets
1) CC (control diet), RL (2.5% dried radish leaves powder), RH (5% dried radish leaves powder) 2) Mineral mixture provides calcium carbonate, 12.5 g: sodium chloride, 2.59 g: potassium citrate, 2.48 g: potassium phosphate, 6.86 g: potassium sulfate, 1.63 g: magnesium oxide, 0.85 g:manganous carbonate 0.02 g: ferric citrate, 0.21 g: zinc carbonate, 0.06 g: cupric carbonate, 0.01 g: potassium iodate, 0.00035 g: sodium selenite, 0.00036 g: chromium potassium sulfate, 0.01 g: ammonium paramolybdate, 0.0003 g: sodium meta-silicate, 0.05 g: libitiumchloride, 0.0006 g: boric acid, 0.003 g: sodium flouride, 0.002 g: nickel carbonate, 0.001 g: ammonium vanadate, 0.0002 g: sucrose finely powdered, 7.73 g 3) Vitamin mixture provides thiamin HCl, 0.006 g: riboflavin, 0.006 g: pyridoxine HCl, 0.007 g: nicotinic acid, 0.03 g: calcium pantothenate, 0.016 g: folic acid, 0.002 g: D-biotin, 0.0002 g: vitamin B12, 0.025 g: vitamin A palmitate, 0.008 g: DL-alpha tocopheryl acetate, 0.15 g: vitamin D3 0.002 g: vitamin K, 0.00075 g: sucrose finely ground, 9.75 g
Table 2
The concentration of total flavonoids and total phenols in dried radish leaves and various fruits (mg/100 g)
Table 3
Total food intake, initial body weight, body weight gain and food efficiency ratio (FER)
1) Values are Means ± SEM for 6 rats per group 2) Values with different superscripts are significantly different in the same species as assessed by ANOVA test (p < 0.05)
WCC: Wistar, control diet, WRL: Wistar, 2.5% dried radish leaves powder, WRH: Wistar, 5% dried radish leaves powder, SCC: SHR, control diet, SRL: SHR, 2.5% dried radish leaves powder, SHR: SHR, 5% dried radish leaves powder
NS: Not significant *: p < 0.05 vs Wistar counterpart
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