Reagent antibodies |
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Acute HLN |
23 Abs CD2, CD3, CD5, CD7, CD10, CD11c, CD13, CD14, CD19, CD20, CD22, CD33, CD34, CD41a, CD45, CD56, CD64, CD117, cCD3, cCD79a, HLA-DR, MPO, TdT |
19 Abs (+3) CD3, CD5, CD7, CD10, CD11c, CD13, CD14, CD19, CD20, CD33, CD34, CD45, CD56, CD64, CD117, cCD3, cCD79a, HLA-DR, MPO (+ if needed, TdT, cCD22, CD61) |
32 Abs CD1a, CD2, CD4, CD5, CD7, CD8, CD10, CD11c, CD13, CD14, CD19, CD20, CD33, CD34, CD36, CD38, CD41b, CD42, CD45, CD56, CD61, CD64, CD71, CD117, CD123, CD235a, cCD3, cCD22, cCD79a, HLA-DR, MPO, TdT |
23 Abs CD2, CD5, CD7, CD10, CD11c, CD13, CD14, CD19, CD20, CD33, CD34, CD41a, CD45, CD56, CD64, CD71, CD79a, CD117, cCD3, cCD22, HLA-DR, MPO, TdT |
21 Abs CD2, CD3, CD5, CD7, CD10, CD13, CD14, CD19, CD20, CD22, CD33, CD34, CD41, CD45, CD56, CD61, CD64, CD117, HLA-DR, MPO, TdT |
20 Abs (+2) CD3, CD5, CD7, CD10, CD13, CD14, CD19, CD20, CD33, CD34, CD41, CD45, CD56, CD117,cCD3, cCD22, cCD79a, HLA-DR, MPO, TdT (+ if needed, CD11c, CD64) |
- AML: 18 Abs CD2, CD7, CD10, CD13, CD14, CD15, CD19, CD33, CD34, CD41, CD45, CD56, CD64, CD117, cCD3, cCD22, HLA-DR, MPO if MPAL: 22 Abs (4 Abs added CD20, cIgM, sIgM, TdT) - ALL: 16 Abs CD2, CD3, CD5, CD7, CD10, CD13, CD19, CD20, CD33, CD34, CD45, cCD3, cCD22, cIgM, sIgM, TdT if T-ALL: 18 Abs (2 Abs added CD4, CD8) |
- 18 Abs (+4) CD3, CD10, CD13, CD14, CD19, CD33, CD34, CD45, CD64, CD66c, CD71, CD117, cCD3, cCD22, cCD79a, HLA-DR, MPO, TdT (+ if needed, CD56, CD123, CD61, CD41) if T-ALL: 26 Abs (8 Abs added CD1a, CD2, CD4, CD5, CD7, CD8, TCRαβ, TCRγδ) |
Chronic HLN |
18 Abs CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD14, CD19, CD20, CD23, CD43, CD45, CD56, CD103, FMC7, sIgκ, sIgλ |
18 Abs CD3, CD4, CD5, CD8, CD10, CD11c, CD19, CD20, CD23, CD38, CD43, CD45, CD56, CD103, FMC7, sIgκ, sIgλ, TCRγδ |
23 Abs CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD49d, CD79b, CD138, FMC7, ZAP70, sIgκ, sIgλ, TCRαβ, TCRγδ |
16 Abs CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD14, CD19, CD20, CD23, CD45, CD56, FMC7, sIgκ, sIgλ |
18 Abs CD2, CD3, CD5, CD7, CD10, CD19, CD20, CD22, CD23, CD34, CD45, CD56, CD138, FMC7, HLA-DR, sIgκ, sIgλ, TdT |
16 Abs CD3, CD4, CD5, CD7, CD8, CD10, CD13, CD19, CD20, CD23, CD33, CD34, CD45, CD56, cCD79a, TdT |
16 Abs CD2, CD3, CD5, CD7, CD10, CD19, CD20, CD22, CD23, CD45, CD56, cCD3, FMC7, sIgκ, sIgλ, TdT |
- B cells: 15 Abs CD5, CD10, CD11c, CD19, CD20, CD22, CD23, CD25, CD38, CD45, CD79b, CD103, FMC7, sIgκ, sIgλ - T/NK cells: 26 Abs CD1a, CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD13, CD14, CD16, CD25, CD26, CD28, CD30, CD33, CD45, CD45RA, CD56, CD57, CD94, CD103, CD279, cCD3, TCRγδ, TdT |
PCN |
NP |
12 Abs β2-MG, CD19, CD27, CD28, CD38, CD45, CD56, CD81, CD117, CD138, cIgκ, cIgλ |
7 Abs CD19, CD38, CD45, CD56, CD138, cIgκ, cIgλ |
7 Abs CD19, CD38, CD45, CD56, CD138, cIgκ, cIgλ |
6 Abs CD19, CD45, CD56, CD138, cIgκ, cIgλ |
5 Abs CD19, CD38, CD45, CD56, CD138 |
5 Abs CD19, CD38, CD45, CD56, CD138 |
9 Abs CD19, CD28, CD38, CD45, CD56, CD117, CD138, cIgκ, cIgλ |
MRD |
- B-ALL:12 Abs CD10, CD19, CD20, CD22, CD34, CD38, CD45, CD58, CD66c, CD73, CD123, CD304 - CLL: 8 Abs CD5, CD19, CD20, CD43, CD45, CD79b, CD81, ROR1 - MM: 9 Abs CD19, CD27, CD38, CD45, CD56, CD81, CD117, CD138, CD200 |
MM: 10 Abs CD19, CD27, CD38, CD45, CD56, CD81, CD117, CD138, cIgκ, cIgλ |
B & T-ALL: 14 Abs CD1a, CD2, CD5, CD7, CD10, CD13 (or CD33), CD19, CD20, CD22, CD34, CD45, CD79a, cCD3, TdT |
- B-ALL: 6 Abs CD10, CD19, CD34, CD20, CD38, CD45 - T-ALL: 6 Abs CD3, CD4, CD7, CD8, CD34, CD45 - MM: 7 Abs CD19, CD38, CD45, CD56, CD138, cIgκ, cIgλ |
NP |
- B-ALL: 7 Abs CD10, CD19, CD20, CD34, CD38, CD45, CD58 - T-ALL: 6 Abs CD3, CD4, CD7, CD8, CD34, CD45 - MBCN: 5 Abs CD5, CD19, CD20, CD23, CD45 |
B-ALL: 7 Abs (+1 or 2) CD10, CD19, CD20, CD34, CD38, CD45, CD58 (+ if needed, CD13 and/or CD33) |
- B-ALL: 12 Abs CD10, CD19, CD20, CD34, CD38, CD45, CD58, CD66c, CD73, CD81, CD123, CD304 - MCL: 10 Abs CD3, CD5, CD19, CD20, CD23, CD45, CD62L, CD200, sIgκ, sIgλ - MM: 10 Abs CD19, CD27, CD38, CD45, CD56, CD81, CD117, CD138, cIgκ, cIgλ |
Fluorophores |
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Acute HLN |
4 colors APC, FITC, PE, PerCP |
8 colors APC, APC-H7, FITC, PE, PE-Cy7, PerCP-Cy5.5, V450, V500 |
9 colors APC, APC-A750, FITC, KO, OC515, PB, PE, PE-Cy7, PerCP-Cy5.5 |
4 colors FITC, PC5, PC7, PE |
4 colors FITC, PC5, PC7, PE |
5 colors APC-A750, FITC, PC5, PC7, PE |
AML/MPAL: 4 colors APC, FITC, PE, PerCP - ALL: 5 colors APC, APC-Cy7, FITC, PE, PerCP |
8 colors APC, APC-H7, BV421 (BV450, PB), FITC, PE, PE-Cy7, PerCP-Cy5.5 (PerCP), V500 (BV510) |
Chronic HLN |
4 colors APC, FITC, PE, PerCP |
8 colors APC, APC-H7, FITC, PE, PE-Cy7, PerCP-Cy5.5, V450, V500 |
8 colors APC, APC-A750, FITC, OC515, PB, PE, PE-Cy7, PerCP-Cy5.5 |
4 colors FITC, PC5, PC7, PE |
4 colors FITC, PC5, PC7, PE |
5 colors APC-A750, FITC, PC5, PC7, PE |
4 colors APC, FITC, PE, PerCP |
8 colors APC, APC-H7, BV421 (BV450, PB), FITC, PE, PE-Cy7, PerCP-Cy5.5 (PerCP), V500 (BV510) |
PCN |
NP |
8 colors APC, APC-H7, FITC, PE, PE-Cy7, PerCP-Cy5.5, V450, V500 |
6 colors APC, APC-A750, FITC, PE, PE-Cy7, PerCP-Cy55 |
4 colors FITC, PC5, PC7, PE |
4 colors FITC, PC5, PC7, PE |
5 colors APC-A750, FITC, PC5, PC7, PE |
5 colors APC, FITC, PE, PE-Cy7, PerCP-Cy5.5 |
7 colors APC, BV421 (BV450, PB), FITC, PE, PE-Cy7, PerCP-Cy5.5 (PerCP), V500 (BV510) |
MRD |
- B-ALL: 9 colors APC, APC-A700, APC-A750, ECD, FITC, KO, PC5.5, PC7, PE - B-CLL: 8 colors APC, APC-A750, FITC, KO, PB, PC5.5, PC7, PE - MM: 9 colors APC, APC-A750, ECD, FITC, KO, PB, PC5.5, PC7, PE |
MM: 8 colors APC, APC-C750, BV421, BV510, FITC, PE, PE-CY7, PerCP-Cy5.5 |
ALL: 8 colors APC, APC-A750, FITC, OC515, PB, PE, PE-Cy7, PerCP-Cy5.5 |
ALL & MM: 4 colors FITC, PC5, PC7, PE |
NP |
- B-ALL: 7 colors APC-A700, APC-A750, ECD, FITC, KO, PC5.5, PC7 - T-ALL, MBCN: 5 colors APC-A750, FITC, PC5, PC7, PE |
B-ALL: 7 colors APC, APC-Cy7, BV510, FITC, PE, PerCP, V450 |
- B-ALL: 10 colors APC, BV605, FITC, PE, PE-Cy7, PerCP-Cy5.5, V450, V500, R718, APC-H7 - MCL: 10 colors APC, APC-H7, APC-R700, BV421, BV605, FITC, PE, PE-Cy7, PerCP-Cy5.5, V500 - MM: 8 colors APC, APC-C750, BV421, BV510, FITC, PE, PE-CY7, PerCP-Cy5.5 |
Sample amount/tube |
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Acute HLN |
100 µL |
100 µL |
100 µL |
100 µL |
50 µL |
50 µL |
volume adjustedto 1×106 cells |
volume adjusted (10–500 µL) to 1×106 cells |
Chronic HLN |
100 µL |
100 µL |
100 µL |
100 µL |
50 µL |
50 µL |
volume adjustedto 1×106 cells |
volume adjusted (10–500 µL) to 1×106 cells |
PCN |
NP |
100 µL |
100 µL |
100 µL |
sAb: 100 µL cAb: 50 µL |
50 µL |
volume adjustedto 1×106 cells |
volume adjusted (10–500 µL) to 1×106 cells |
MRD |
1×107 cells |
volume adjustedto ≥1×107 cells |
volume adjustedto 5×105 cells |
100 µL |
NP |
50 µL |
volume adjustedto 1×106 cells |
volume adjusted (10–500 µL) to 1×107 cells |
Amount of reagent antibody/tube |
5 µL |
Depending on fluorophores or Ags - APC, APC-H7, PE-Cy7,PerCP-Cy5.5, V450, V500: 5 µL - FITC, PE: 20 µL - CD34-PerCP-Cy5.5: 20 µL - CD56-V450: 2 µL |
5 µL |
10 µL |
10 µL (Isotype IgG1/G2a: 5 µL) |
Depending on fluorophores - APC-A750: 3 µL - PC5, PC7: 5 µL - FITC, PE: 10 µL |
8 µL - ALL CD10, CD19, CD20: 5 µL - ALL-MRD CD10, CD19, CD20: 5 µL CD58: 2 µL |
Depending on Abs2.5–20 µL |
Acquisition cell count |
Acute HLN |
2×104 cells |
1×105 cells |
≥5×103 cells |
5×103 to 1×104 cells |
- sAb: 1×104 cells - cAb: 5×103 cells |
1×104 cells |
5×104 cells |
2×105 cells |
Chronic HLN |
2×104 cells |
1×105 cells |
≥5×103 cells |
5×103 to 1×104 cells |
- sAb: 1×104 cells - cAb: 5×103 cells |
1×104 cells |
5×104 cells |
2×105 cells |
PCN |
NP |
1×105 cells |
≥5×103 cells |
5×103 to 1×104 cells |
1×105 cells |
1×106 cells |
5×105 cells |
2×105 cells |
MRD |
5×106 cells |
1×107 cells |
≥5×105 cells |
5×103 to 1×104 cells |
NP |
1×106 cells |
1×106 cells |
- B-ALL: 6×106 cells - MM: 1×107 cells |