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INTRODUCTION
Invasive fungal infections (IFIs) are opportunistic pathologies with potentially preventable serious complications, mainly in immunocompromised patients, with high morbidity and mortality rates [1].
Risk factors for the development of IFI include host factors, factors related to the underlying disease, factors associated with treatment, complications [2] and in some cases, environmental factors [3-5].
The overall IFI incidence is 2–16%, depending on transplant characteristics, diagnostic methods, and follow-up [6-8]. The most common etiological agents are Candida spp. and Aspergillus spp. [6], with 60–95% mortality rate in transplant patients [7].
Antifungal prophylaxis has reduced the number of fungal infections in patients who have undergone allogeneic hematopoietic stem cell transplantation (AHSCT) [9]. Different regimens are used in pediatric patients, including fluconazole [10], voriconazole [11, 12], itraconazole [13], caspofungin [14], and posaconazole [15]. Voriconazole has shown an acceptable tolerance profile, manageable and transient adverse events, and adequate antifungal coverage against filamentous fungi [16]. Although prophylaxis has decreased the number of IFI cases, this pathology continues to be a relevant complication in immunocompromised patients [17].
This study aimed to compare the outcome variables of pediatric patients who underwent AHSCT and received antifungal prophylaxis, such as voriconazole with those who received other antifungals at Fundación Valle del Lili, Cali, Colombia, from 2012 to 2018.
MATERIALS AND METHODS
Study population
This was an observational, analytical, retrospective cohort study of pediatric patients with AHSCT between 2012 and 2018. Data were collected from the electronic medical records of all patients aged <18 years who underwent AHSCT at the Fundación Valle del Lili. The exclusion criteria were patients with previous IFI. The Ethics Committee on Biomedical Research approved this study under code 1442.
Definitions
According to the literature, IFIs are proven probable and possible [18]. Proven when it met a patient criterion (bone marrow transplant, neutropenia, prolonged use of corticosteroids or immunosuppressants or carrier of severe congenital immunodeficiency), plus a clinical criterion (tracheo-bronchitis, sinusitis, central nervous system infection, disseminated candidiasis or lower respiratory tract fungal infection), and a microbiological criterion (identification of the agent by microscopic analysis of sterile biopsy or aspirate material, culture of sterile material or blood). Probable when there was a host criterion plus a clinical criterion and microbiological criterion (direct or indirect tests that found fungal elements). Possible if it meets the patient criterion plus at least one clinical diagnosis without microbiological support.
Antifungal prophylaxis: The antifungal agent used between day 0 and day +100 was considered until the IFI development or death. The regimen included intravenous voriconazole at a dose of 8 mg/kg twice a day without a loading dose for children between 2 and 11 years old and adolescents aged 12–14 years with <50 kg body weight, or 4 mg/kg twice a day for adolescents aged 12 to 14 years with ≥50 kg body weight and all adolescents over 14 years [19, 20]. Caspofungin was administered at a dose of 50 mg/m2/day and fluconazole at a dose of 5–7 mg/kg/day. Posaconazole (300 mg/day) Liposomal amphotericin B was administered at a prophylactic dose of 1 mg/kg/day [21]. According to the pharmaceutical recommendation, itraconazole was administered at a starting dose of 5 mg/kg on days 1 and 2 and a continuation dose of 2.5 mg/day. In any adverse event or complication, the drug related to the event should be changed.
The conditioning regimen was classified as myeloablative or reduced in intensity [22]. Graft-versus-host disease (GVHD) prophylaxis was performed according to the transplant type [23-25]. The graft was defined according to the literature [26]. The Glucksberg criteria have been used to grade acute GVHD [27]. The cause of death within +100 days post-AHSCT was defined according to literature [28].
Statistical analysis
A comparative statistical analysis of the incidence of IFI between patients who received initial prophylaxis with voriconazole and those who received other antifungals was performed (total group, N=139). All variables were evaluated for up to 100 days after AHSCT. The comparison groups were selected considering that voriconazole is the most widely used prophylactic antifungal drug in the institution, and other antifungals constituted a smaller proportion.
Continuous variables are expressed as medians and interquartile ranges, and comparisons were performed using the Mann–Whitney test. Categorical variables are expressed as proportions and compared using the chi-square or Fisher’s exact test, as appropriate. Statistical significance was defined as P<0.05. A multivariate analysis was performed with logistic regression that included risk factors with P-values <0.2 and other known factors for IFI, regardless of P-value, using the stepwise backward methodology to select the variables in the final model. Subgroup analysis comparing voriconazole and fluconazole was performed (N=119). Survival analysis and cumulative incidence estimation of IFI were performed using the Kaplan–Meier method. The log-rank test was used to compare the cumulative incidence of IFI between the groups. The statistical software STATA 14 was used for the data analysis.
RESULTS
A total of 139 patients were evaluated and divided into two groups according to the initial antifungal dose. Of these, 96 were administered with voriconazole, and 43 had other antifungals, including fluconazole [23], caspofungin [14], posaconazole [4], and amphotericin B [4]. The median age was 9.7 years (IQR, 4.7–13), and 41% of the patients were women. The most frequent pathology was acute leukemia (63%). The donors were haploidentical (75%), matched (23%), and cord (2%). The stem cell sources were bone marrow (65%), peripheral blood (29.5%), combined bone marrow and peripheral blood (3.5%), and umbilical cord blood (2%). Conditioning was myeloablative in 55% of patients, and 34% used ATG during conditioning.
Prophylaxis for acute GVHD was post-transplant cyclophosphamide-based in 78% and cyclosporine-based in 23% (Table 1). The median neutrophil and platelet graft times were 16 days. In the month before AHSCT, 50% of patients received one or more antifungals, mainly fluconazole and voriconazole. The 48% received one or more antimicrobial therapies, and 24% received steroids (Tables 1, 2). Seven patients had IFI (one proven, and six possible).
Patients with IFI
The 100-day cumulative incidence of IFI was 5%. Seven patients had an IFI. One was proven by bronchoalveolar lavage culture (one Candida parapsilosis), and six were possible. Four patients received prophylactic voriconazole, and three received another antifungal agent. In this group, one (11%) died of multiple organ failure at 15 days (Table 3).
Post-AHSCT outcomes and complications
Acute GVHD grades III–IV occurred in 11% of all patients, and moderate-to-severe mucositis occurred in 43%. Fifty-one percent had other infections, 7% of which were catheter-associated. Primary graft failure occurred in 4.5% of those one with neutrophil and platelet graft failure and six with platelet graft failure. Twenty-four (17%) patients died, of which seven died before 28 days. Of all deaths, three (12.5%) were due to the disease, and 21 (87.5%) were due to AHSCT-related causes (Table 4). The 100-days overall survival (OS) rate was 81% (Fig. 1).
Some of the clinical characteristics of the study showed differences between those who received voriconazole and those who received other antifungal prophylaxis. To control for the differences between these groups, we performed multivariate analysis for IFI diagnosis. We included the following variables: voriconazole prophylaxis, moderate-to-severe mucositis, malignant-based disease, acute GVHD grade III–IV, and myeloablative conditioning regimen. However, there were no statistically significant differences (Table 5); therefore, we performed a subgroup analysis (Table 6). Acute GVHD grade III–IV is a risk factor for IFI, independent of the other variables analyzed.
DISCUSSION
In this single-centered retrospective cohort study, two antifungal prophylaxis regimens (voriconazole or other antifungals) were used from the time of transplantation to 100 days after AHSCT, compared to 139 pediatric patients treated from 2012 to 2018 at a reference center in Cali, Colombia. There were seven cases of post-AHSCT IFI (one proven and six possible). No differences were found in the incidence of IFI between the two prophylaxis groups.
In this study, the 100-day cumulative incidence of IFI was 5%, similar to that reported by Hazar et al. [6], who reported an incidence of 5% after excluding cases of possible IFI. However, our results differed from those with Hovi et al. [7], who reported an incidence of 12%, which is higher than our results.
The pathogen isolated in proven IFI was Candida parapsilosis, similar to that reported in other studies [6-8, 29]. Of the seven patients with IFI, only one (11%) died of multiorgan failure, without direct association with IFI. Our incidence of mortality contrasts with the deaths due to IFI reported in the literature between 20–70% [29, 30]. This may be due to the small number of IFI cases in the study population.
The use of an antifungal prophylaxis protocol in transplant patients helps protect them against infection during the period of immunosuppression and neutropenia [17], which typically includes the first +100 days. Therefore, individualized antifungal prophylaxis based on patient characteristics and local epidemiology is essential to identify the groups most at risk of infection and to provide the best option for each patient. Voriconazole is the most widely used prophylactic antifungal agent (69% of patients) because of its broad-spectrum activity, especially against filamentous fungi such as Aspergillus, which distinguishes it from fluconazole [11].
This study has some limitations. First, this was a retrospective design and data collection from electronic medical records could have lacked relevant data. Second, this had a short follow-up period, which could have affected the study results. Third, it was conducted only in a single institution in the country. However, this study revealed important information about IFI cases occurring in a transplant unit in our population.
In conclusion, we found no differences in the incidence of IFI between patients who received antifungal prophylaxis with voriconazole or other antifungal medications. Studies with larger sample sizes are required to identify independent risk factors for post-transplant IFI. In the Azoles group, it was observed that the presence of GVHD III–IV was an independent risk factor for IFI in the multivariate analysis performed for the subgroup.
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