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Introduction
Pluripotent stem cells (PSCs), displaying unlimited proliferation ability and the multi-lineage differentiation potential, have emerged as an alternative and reproducible source of primary cells with therapeutic interest (1). The improvement of PSCs-based liver modeling systems can be exemplified by the transition from monolayer hepatic-like cells (2-5) to the 3D liver micro-tissues with improved functionality (6, 7). However, models in these studies are lack of a number of critical cell types such as biliary epithelial cells. Cholangiocytes line the biliary tracts, and facilitate the transport, modification, and secretion of bile from the liver into the luminal gastrointestinal tract (8, 9). These functions have been proved essential to maintain cell viability and functionality of the hepatic counterpart both in vivo and in vitro (10, 11). To date, despite several methods could respectively differentiate human PSCs into functional hepatocyte-like cells (2-5) or cholangiocyte-like cells (12-14), none are able to generate a hepatic organoid containing biliary structures, which is now considered as a critical hurdle to overcome for allowing the organoids being more refined and mature (15).
To address this issue, we have recently established a hPSC-derived hepatobiliary organoids (HBO) system (16). Herein, we present an updated version of our previous described 45-day protocol in detail, whereby a timing of 35-day protocol to obtain the mature HBO. Furthermore, a hepatobiliary culture medium is developed to functionally maintain the organoids for more than 1.5 months, therefore enabling the in vitro applications that require a long-term manipulation, such as repeated dosing and disease modelling.
Materials and Methods
Pluripotent stem cells
The human embryonic stem cells we used are H1 and H9 cells (WA01 and WA09, Wicell Research Institute, cat. no. WB16217 and RB40917). The human iPSC lines are UC and WD cells (UC-S0730C11 and WD1-KSR-3, Chunshui Bioscience & Technology, 0109 and 0105).
Differentiation of hPSCs into endoderm and mesoderm
PSCs were plated onto pre-coated 3 μg/ml laminin 521-111 (mixed with a ratio of 1：3, BioLamina, LN521- 02 and LN111-02). At the day before differentiation, cells were seeded at the density of 2.5×105 cells viable cells/cm2, which would reach 90% confluency after attachment for the initiation of differentiation. Pre-stimulation was conducted at 90∼95% confluency by replacing mTeSR1 (STEMCELL Technologies, 85850) with Pre-endoderm and -mesoderm Medium (Pre-EMM) for 5 hours: RPMI 1640 containing 2% B27 supplement (minus insulin) (Life Technologies, A1895601), 100 ng/ml Activin A (PeproTech, 120-14P), and 20 ng/ml BMP4 (PeproTech, 120-05ET). The medium was then replaced with EMM-1 (5% mTESR1, 2% B27 supplement (minus insulin), 100 ng/ml Activin A, and 20 ng/ml BMP4) for 2 days and EMM-2 (5% mTeSR1, 2% B27 supplement (minus insulin), 100 ng/ml Activin A) for another 2 days.
Differentiation of endoderm into hepatoblast-like cells
The cultures at day 5 were placed in Progenitor Medium (PM) for 5 days: RPMI1640 containing 5% mTeSR1, 2% B27 supplement (Gibco, 17504044), 30 ng/ml FGF4 (Peprotech, 100-31) and 20 ng/ml BMP2 (Peprotech, 120-02C). The medium was then replaced with Expansion Medium (EM) for additional 5 days: RPMI1640 containing 5% mTeSR1, 2% B27 supplement, 20 ng/ml KGF (Peprotech, 100-19), 20 ng/ml HGF (Peprotech, 315-23), 1% GlutaMAX (Gibco, 35050061) and 1% NEAA (Gibco, 11140050). No transfer operation was needed.
Hepatobiliary differentiation and maturation
The cultures at day 16 were placed in Hepato-biliary Differentiation Medium (HBDM) for 10 days: HCM (Lonza, CC-3199 & CC-4182) containing 20 ng/ml OSM (Peprotech, 300-10), 5% Cholesterol＋-MIX (ProbeChem, PC-35742), 1% GlutaMAX and 1% NEAA. The medium was then replaced with Hepato-biliary Maturation Medium (HBMM) for additional 10 days: HCM containing 20 ng/ml OSM, 5% Cholesterol＋-MIX, 0.5 μM Dexametha-some (Sigma-Aldrich, D1756), 2 μM Vitamin K2 (Selleck, S5082), 5 μM Lithocholic acid (Selleck, S4003). No transfer operation was needed.
In vitro Maintenance of HBOs
The HBOs were placed in Hepato-biliary Culture Medium (HBCM): William’s E medium (Gibco, A1217601) containing 2.5% Cholesterol＋-MIX, 1% ITS＋3 (Sigma- Aldrich, I2771), 10% KSR (Gibco, 10828028), 20 ng/ml HGF, 20 ng/ml EGF (Peprotech, 100-47), 10 ng/ml VEGF (Peprotech, 100-20C), 10 ng/ml FGF2 (Peprotech, 100- 18B), 1% GlutaMAX and 1% NEAA, which can functionally maintain the HBOs for about 50 days. No transfer operation was needed.
Immunofluorescence
Monolayer staining: the cultures displayed monolayer form (differentiation day 0, 4, 9, 15) were fixed with 4% paraformaldehyde (PFA) for 20 mins at room temperature (RT), and permeabilized with 0.2% Triton X-100 (Sigma- Aldrich, T8787) or cold 100% methanol. After blocking with 10% donkey serum (The Jackson Laboratory, 017- 000-121) for 1 hour, the cells were incubated with primary antibodies in 1% donkey serum at 4℃ overnight, and secondary antibodies in 1% donkey serum for 45 mins at RT. Finally, DAPI (Sigma-Aldrich, D8417) was used to counterstain the nuclei. Samples were washed 4 times with PBS for 5 min at RT after primary and secondary antibody and DAPI staining.
The 3D structure staining was performed as follows: 3D samples (differentiation day 25, 35) were fixed with 4% PFA at RT overnight, washed with normal saline (0.85% NaCl) and then embedded in Richard-Allan Scientific HistoGel (Thermo Scientific, HGSK-2050-1). The HistoGel block was dehydrated, embedded in paraffin and sectioned at 4 μm. Paraffin-embedded sections were dewaxed with xylene, rehydrated, placed in Tris-EGTA-buffer (pH 9.0) or citrate buffer (pH 6.0) and subjected to heat mediated antigen retrieval (Microwave) for 20 mins. The subsequent staining steps are described above (as per monolayer staining).
All immunofluorescence images were acquired using a Leica TCS-SP5 confocal microscope. LAS X software (Leica) was used for image processing. A complete list of the primary and secondary antibodies used is shown in Supplementary Table S1.
Flow cytometry
Samples were incubated with TrypLE (Gibco, 12604013) at 37℃ until the cells began to dissociate. Cells were centrifuged and resuspended in 2% PFA at 4℃ for 20 mins and centrifuged and resuspended in flow cytometry buffer (PBS containg 5% FBS and 0.1% Triton X-100). Cells were then incubated with primary antibodies or control IgGs for 30 mins on ice, and secondary antibodies for 20 mins on ice. Analysis was performed using a FACS LSR Fortessa flow cytometer (BD Biosciences). IgG controls were used to set gates for positive cells.
Albumin and urea assay
Albumin and Urea production were detected using ELISA kit (Bethyl Laboratories, E88-129) and Quantichrom Urea assay Kit (Bioassay Systems, 360-DIUR-100), respec-tively. Cells were incubated in fresh medium for 24 hours and subsequently the cell supernatant was collected at different time points and analyzed for albumin secretion. Cells were either trypsinized and counted using a hemocytometer, or lysed for protein content assay with a BCA Protein Assay Kit (Thermo Scientific, 23225).
CYP3A4 activity and inductivity
CYP3A4 activity was measured using p450-Glo assay kit (Promega, V9001). Briefly, the organoids, cryopreserved human hepatocytes (Lonza, HUCPI) and HepG2 cells (ATCC, HB-8065) were rinsed in HCM and incubated with the luminescent substrate (3 mM luciferin-IPA) and then cells were diluted in the same medium for 1 hour at 37℃. Following incubation, supernatants were processed in accordance with the manufacturer’s instructions. Luminescence was measured using a luminometer (GloMax Discover, Promega).
For CYP3A4 induction assays, cultures were treated with 25 μM rifampin (Sigma-Aldrich, R3501) or 0.1% vol/vol DMSO control dissolved in HCM for 48 hours, followed by quantification of CYP3A4 activity using the luminescent assays described above. Inducer compounds were replaced daily.
Alkaline phosphatase staining
Alkaline phosphatase (ALP) staining was carried out using the BCIP/NBT color development substrate (5-bromo- 4-chloro-3-indolyl-phosphate/nitro blue tetrazolium) (Pro-mega, S3771) according to the manufacturer’s instructions.
Measurement of γ-glutamyl transferase activity
γ-glutamyl transferase activity (GGT) was measured in triplicate using the MaxDiscovery GGT Enzymatic Assay Kit (Bio Scientific, 5601-01) in accordance with the manufacturer’s instructions. Substrate only was used as the negative control. Human intrahepatic biliary epithelial cells (ScienCell, 5100) was used as positive control.
Results
The HBO was produced from hPSC through a step-wise differentiation process that mimics hepatobiliary develop-ment. The differentiation stages with corresponding morphological features were shown in Fig. 1.
Differentiation of hPSCs into bipotent hepatoblasts
At the day before differentiation, the pluripotency of human PSC was examined by immunofluorescence and flow cytometry: pluripotent marker such as OCT4 and NAONG were co-stained by more than 98% cells (Fig. 2A).
As described previously, the signaling from mesodermal derivatives was important to achieve the co-differentiation of hepatic and biliary lineages (16). Therefore, the first target in this protocol is to simultaneously trigger endodermal and mesodermal fate. When the confluence of PSCs at day 0 reached 90∼95%, a 5-hour pre-stimulation was performed using pre-EMM, leading to detachment and death for a part of cells, as typical morphology of the culture shown in Fig. 1B (Day 0∼5 h). After that, EMM-1 and EMM-2 were sequentially used for both 2 days, to promote differentiation of the two germ layers. By the end of day 4, More than 70% cells co-expressed definitive endoderm marker SOX17 and FOXA2 (17), over 10% cells expressed mesoderm marker BRA (18), whereas very few cells expressed ectoderm marker NESTIN (Fig. 2B) (19).
For next stage, PM was used for 5 days to promote the differentiation of hepatic progenitors. Immunofluore-scence and flow cytometry analyses showed more than 60% of the cells co-expressed early hepatic marker HNF4A and AFP (20) by day 9. EM was then applied for additional 5 days to expand the progenitors, making the proportion of AFP＋ population increased to over 80%. In the meantime, a minimum of 50% of cells co-expressed AFP and SOX9 (biliary marker) (Fig. 2D), which represents the feature of bipotent hepatoblasts (21).
Differentiation of bipotent hepatoblasts into HBOs
At day 16, HBDM was used for 10 days to differentiate hepatoblast-like cells into early HBOs. During this stage, some CK19＋ biliary structures began to appear, and the underlying ALB＋ hepatic lineage exhibits distinct nuclei/nucleoli and more cobblestone-like morphology, as identified in Fig. 1B (Day 25) and Fig. 2E. By day 25, over 70% of the cells expressed ALB, while over 15% of the cells expressed CK19 by flow cytometric analysis.
To promote the maturation of HBOs, HBMM was used for another 10 days. For morphology, a clearly more clustered appearance of hepatic cells and a higher number of biliary cysts could be observed during this stage (Fig. 1B (Day 35). The proportions of the two lineages would be overall stable by flow cytometric analysis (Fig. 2F). In addition, mature hepatic marker CYP3A4 and biliary marker CK7/Ac-α-tubulin/CFTR (22) can be identified at the end of this stage (Fig. 2F, Supplementary Fig. S1).
In vitro culture of HBO
In this stage, HBCM was applied to culture the HBOs, which could functionally maintain the organoids for about 50 days in vitro. Functional examination can be performed during this period. As shown in Fig. 3A, the albumin secretion of day-35 HBOs was comparable to that in cryopreserved human adult hepatocytes (7.21±1.20 vs 12.92±2.60 μg/ml/day/mg protein, p=0.09) when normalized using protein content; whereas the urea production was lower than primary hepatocytes (49.21±8.47 vs 112.44±16.50 μg/ml/day/mg protein, p<0.05). hPSCs showed no production of albumin and urea. The CYP3A4 activity of HBOs was not decreased from day 35 to day 80 (0.21±0.06 vs 0.19±0.04 relative light unit/ml/day/mg protein, p=0.79) (Fig. 3B). In addition, when dosing with inducer such as rifampin, both day-35 and day-80 HBOs demonstrated significant induction (over DMSO-treated control by 1.4–2.6-fold and 1.5–2.8-fold, p<0.05 and p< 0.05, respectively). Whereas primary hepatocytes were more robustly induced (2.2-3.4-fold, p<0.01). For biliary function, ALP activity was confirmed, as shown in Fig. 3C. Moreover, GGT activity of day-35 and day-80 HBOs was comparable to that in human intrahepatic biliary epithelial cells (Fig. 3D).
Discussion
The research on the mechanisms of human hepatobiliary development is limited by the poor access to human fetal tissue, and lower accuracy of animal models (23). Our hPSC-derived HBO system could address some of these challenges, as it relies on a stepwise differentiation procedure that closely mimics the embryonic hepatogenesis, including the generation of hPSC-derived hepatoblast-like cells, specification of this population to hepatocytic and biliary fate, and then the induction and formation of the complex organoids with hepatic clusters and biliary structures. In this regard, researchers can use our protocol to produce substantial numbers of cells corresponding to different embryonic stages of the liver, facilitating the mechanistic studies on hepatobiliary specifi-cation.
Mutations in hepatobiliary genes have been revealed in many liver diseases such as non-alcoholic fatty liver disease (24) and Alagille syndrome (25). However, rodent models often fail to recapitulate human pathophysiology (26, 27). Our approach can therefore be utilized in combination with genome editing technologies such as CRISPR/ Cas9 (28) to produce isogenic hPSC lines that differ only by specific mutations, and then differentiate them into HBOs for the examination of pathological phenotypes and discovery of therapeutic strategies (29).
Besides providing a better model for human liver development and diseases, the HBOs may be used for toxicity screening and drug development. Methods for in vitro culture of human hepatocytes (30), as wells as the PSC-based hepatocyte-like cells (31) have been developed to address this limitation. The HBO system presents significantly more complex liver structures when compared with 2D monolayer hepatocytes, thus, possesses a higher potential to precisely predict drug metabolism and toxicity. Compared to our previous 45-day differentiation system (16), the current protocol shortens the differentiation to 35 days, and more importantly, it enables HBO in vitro maintenance for more than 1.5 months without functional loss and meets the requirement of the applications with long-term intervention and analysis.
Naturally, there are a few limitations for current system. One of the important considerations is the maturity of the generated HBOs, which exhibit a mixed functional phenotype between fetal and adult liver (referred to the expression profile of hepatobiliary markers (16), ALB and urea production, CYP3A4 activity and inducibility, and GGT activity). Therefore, before modeling adult hepatobiliary disorders, HBOs should be examined for the presence of mature markers and functionality. Recently, Mun et al. (32) reported that the R-spondin approach could be applied to expand the hPSC-derived hepatic lineage under 5% hypoxia condition. The expandable potential of our system may be investigated by setting hypoxic condition.
Our differentiation method worked well for all the human ES (H1 and H9) and iPS (UC-S0730C11 and WD1- KSR-3) cell lines examined in this study. Owing to intrinsic variability in human iPS and ES cell lines (33-35), however, researchers may need to adjust the protocol depending on the cell lines they used. As shown in the Troubleshooting table (Table 1), some of the modifications may include the use of different concentrations of the cytokines, adjusting the timing of induction for each differentiation stage, as well as altering concentration or the type of cell culture substrate.
In summary, we herein present a detailed protocol for differentiation of hPSC into functional HBOs. This stepwise differentiation closely mimics human hepaobiliary specification, in which the HBOs can be functionally maintained in vitro up to day 85. We expect these HBOs can be used as an alternative cell source for studies of human hepatobiliary regeneration, disease mechanism, toxicology studies, or drug development.
Supplementary Materials
Supplementary data including one table and one figure can be found with this article online at https://doi.org/10. 15283/ijsc20152
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