Salivary alpha-amylase as a stress biomarker in diseased dogs

Hwa-Ran Hong; Ye-In Oh; Young Jun Kim; Kyoung-Won Seo

doi:10.4142/jvs.2019.20.e46

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Hong, Oh, Kim, and Seo: Salivary alpha-amylase as a stress biomarker in diseased dogs

Short Communication Internal Medicine

J Vet Sci 2019;20(5):e46.

Published online: 4 September 2019

DOI: https://doi.org/10.4142/jvs.2019.20.e46

Salivary alpha-amylase as a stress biomarker in diseased dogs

Hwa-Ran Hong¹, Ye-In Oh¹, Young Jun Kim², Kyoung-Won Seo^1,^*

¹Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea

²BioMedIT Group, Electronics and Telecommunications Research Institute, Daejeon 34129, Korea

^*Corresponding author: Kyoung-Won Seo Department of Veterinary Internal Medicine, College of Veterinary Medicine, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Korea. E-mail: kwseo@cnu.ac.kr

Received 18 May 2019 Revised 16 July 2019 Accepted 26 July 2019

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Salivary alpha-amylase (sAA) is a stress biomarker in human diseases, but there are no reports of sAA measurements in diseased dogs. This study measured the sAA and serum alpha-amylase (AA) levels in 16 healthy dogs and 31 diseased dogs using a kinetic enzyme assay to assess the stress status. The sAA and serum AA levels were significantly higher in the diseased dogs than in healthy dogs (p < 0.05), but there was no correlation between the 2 groups (r = 0.251, p = 0.089). This suggests that sAA can be useful as a stress biomarker in diseased dogs.

Keywords: Alpha-amylase, dogs, saliva, serum, stress

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Fig. 1.

Relationship between the sAA and serum AA in all dogs. No correlation was observed between the alpha-amylase activities of the serum and saliva according to Spearman correlation analysis (r = 0.251, p = 0.089). sAA, salivary alpha-amylase; AA, alpha-amylase.

Table 1.

Alpha-amylase activity (U/L) in the saliva and serum samples from healthy and diseased dogs analyzed using an enzyme kit

Samples	Healthy dogs	Diseased dogs	p value
Saliva	37.25 (15.40–52.68)	72.71 (57.15–122.06)	< 0.05
Serum	725.995 (501.79–870.92)	942.97 (776.34–1,121.14)	< 0.05

Significant differences were expressed as p values by comparing the group of healthy dogs with that of diseased dogs using the Mann-Whitney U test.

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