Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53

Ri Zhe Zhu; Bing Si Li; Shang Shang Gao; Jae Ho Seo; Byung-Min Choi

doi:10.4196/kjpp.2021.25.4.297

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Zhu, Li, Gao, Seo, and Choi: Luteolin inhibits H2O2-induced cellular senescence via modulation of SIRT1 and p53

Original Article

Korean J. Physiol. Pharmacol. 2021;25(4):297-305.

Published online: 1 July 2021

DOI: https://doi.org/10.4196/kjpp.2021.25.4.297

Luteolin inhibits H₂O₂-induced cellular senescence via modulation of SIRT1 and p53

Ri Zhe Zhu^#, Bing Si Li^#, Shang Shang Gao, Jae Ho Seo^*, Byung-Min Choi^*

Department of Biochemistry, Wonkwang University School of Medicine, Iksan 54538, Korea

^*Correspondence Jae Ho Seo, E-mail: bionian9@wku.ac.kr, Byung-Min Choi, E-mail: bmchoi@wku.ac.kr

Equal contributor:

^# These authors contributed equally to this work and should be considered as co-first authors.

Received 7 September 2020 Revised 24 February 2021 Accepted 5 April 2021

(open-access):

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Luteolin, a sort of flavonoid, has been reported to be involved in neuroprotective function via suppression of neuroinflammation. In this study, we investigated the protective effect of luteolin against oxidative stress-induced cellular senescence and its molecular mechanism using hydrogen peroxide (H₂O₂)-induced cellular senescence model in House Ear Institute-Organ of Corti 1 cells (HEI-OC1). Our results showed that luteolin attenuated senescent phenotypes including alterations of morphology, cell proliferation, senescence-associated β-galactosidase expression, DNA damage, as well as related molecules expression such as p53 and p21 in the oxidant challenged model. Interestingly, we found that luteolin induces expression of sirtuin 1 in dose- and time-dependent manners and it has protective role against H₂O₂-induced cellular senescence by upregulation of sirtuin 1 (SIRT1). In contrast, the inhibitory effect of luteolin on cellular senescence under oxidative stress was abolished by silencing of SIRT1. This study indicates that luteolin effectively protects against oxidative stress-induced cellular senescence through p53 and SIRT1. These results suggest that luteolin possesses therapeutic potentials against age-related hearing loss that are induced by oxidative stress.

Keywords: Cellular senescence, Hydrogen peroxide, Luteolin, Sirtuin 1, Tumor suppressor protein p53

INTRODUCTION

Cellular aging or senescence, a process which irreversible cell cycle arrest was drove, is a sort of cellular response to various types of stresses including oxidative stress, DNA damage, metabolic alterations and oncogene activation [1]. Oxidative stress is thought to be the most redundant source to trigger cellular aging or senescence in the point of the molecular basis on aging [2,3]. Chronic oxidative stress stems from an imbalance of redox status [4,5]. One of the characteristics of aging is the decline in the level of anti-oxidants during senescence [6], which indicates the physiological importance of oxidative stress in cellular senescence and aging.

DNA damage is associated with the modulation of Saccaromyces cerevisiae chromatin silencing factor Sir2 (silent information regulator-2), which is one of the main factors that determines life span. Mammalian sirtuin 1 (SIRT1), nicotinamide adenosine dinucleotide (NAD)-dependent protein deacetylase, highly conserved with Sir2, participates in regulation of many cellular events including cell cycle, senescence, apoptosis and metabolism by interacting with key molecules for those events like p53, signal transducer and activator of transcription (STAT3) and Peroxisome proliferator-activated receptor gamma coactivator (PGC1α) [7 -9]. A previous study has shown that SIRT1 neutralizes progressive multifocal leukoencephalopathy (PML)-induced premature senescence by deacetylation of p53 in mouse embryo fibroblasts [7]. Recently many evidences have been reported that inhibition or downregulation of SIRT1 induces premature senescence-like phenotype through the augmentation of p53 acetylation in human cancer and endothelial cells [10]. Reversely, overexpression of SIRT1 makes cells more protective from senescence-related physiological alterations in cells [8]. Therefore, it would be potential target to be able to regulate redox and SIRT1 expression for deferring aging process.

Luteolin is a highly abundant natural flavonoid that exists in celery, green pepper and chamomile. Luteolin has been reported to have anti-inflammatory, anti-oxidant and anti-carcinogenic activities [11 -13]. It has been reported as a potent inhibitor to be able to restrain tumor necrosis factor-α (TNF-α) and Interleukin 6 (IL-6), and nitric oxide expression induced by lipopolysaccharide [11]. It also has been considered as an anticancer agent through its inhibitory role for metastasis and angiogenesis, enhancing role of cell cycle arrest and apoptosis [14]. A latest study has indicated that luteolin ameliorates cisplatin-induced acute kidney injury in mice by regulating p53-dependent renal tubular cell apoptosis [15]. It has also been shown that luteolin inhibits microglia and alters hippocampal-dependent spatial working memory in aged mice and exhibits protective roles in neurodegenerative diseases [16,17]. However, there is a lack of direct evidence showing the association between luteolin and the observed anti-aging effect in vitro as well as the relevant molecular mechanisms. In this study, we investigated the inhibitory effect of luteolin against hydrogen peroxide (H₂O₂)-induced cellular senescence and the molecular mechanism of its inhibitory action with focus on modulation of SIRT1 and p53.

METHODS

Materials

Luteolin, 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-Gal) and H₂O₂ were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against the following proteins were used: phospho-p53 (p-p53, Cell Signaling Technology, 9284), p21 (Santa Cruz Biotechnology, sc-397), Sirt1 (Merck Millipore, 07-131), β-actin (Santa Cruz Biotechnology, sc-1616), phospho-JNK (p-JNK, Cell Signaling Technology, 9251), phospho-ERK (p-ERK, Cell Signaling Technology, 9101), and phospho-p38 (p-p38, Cell Signaling Technology, 9211). The specific siRNA for p53 (sc-29436) and SIRT1 (sc-40987) was purchased from Santa Cruz Biotechonology (Santa Cruz, CA, USA). Unless indicated otherwise, all other chemicals were obtained from Sigma-Aldrich.

Cell culture

House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, derived from long-term cultures of Immortomouse cochlea, are extremely sensitive to ototoxic drugs. HEI-OC1 cells possess hair cell-like properties, express several specific markers, including Math 1 and Myosin 7a [18]. HEI-OC1 cells obtained from Center for Metabolic Function Regulation, Department of Microbiology, Wonkwang University (Iksan, Korea). Cells were cultured with high-glucose DMEM containing 10% FBS and stored in a humidified incubator (33 degree, 5% CO₂) without antibiotics.

Cell viability and cell proliferation by MTT assay

Cell viability and cell proliferation was performed as Yu et al. [19] and Lee et al. [20] described, respectively. Briefly, Cells (4 × 10⁴/well) were seeded in 96-well plates and treated with luteolin and H₂O₂ as described in figure legends (Fig. 1A, D). Then 20 μl of MTT (3-[4,5-dimethythiazol-2-yl]-2,5-diphenyltetrazolium bromide, a tetrazolium salt) test solution was added and incubated for 4 h at 37°C in a humidified incubator with 5% CO₂. The absorbance was measured at a wavelength of 490 nm using a SpectraMax M3 instrument (Molecular Devices, Sunnyvale, CA, USA).

Senescence-associated β–galactosidase (SA-β-gal) staining

The proportion of SA-β-gal positive cells was determined as described by Dimri et al. [21]. HEI-OC1 cells were washed twice with phosphate-buffered saline (PBS) and fixed with 3.7% formaldehyde in PBS for 10 min at room temperature. After washing with PBS, cells were incubated with β-galactosidase reagent (1 mg/ml X-gal, 40 mM citric acid/sodium phosphate buffer, pH 6.0, 5 mM potassium ferrocyanide/potassium ferricyanide, 150 mM NaCl, 2 mM MgCl₂) at 37°C. The cells were washed twice with PBS, then we counted old cells (blue) and total cells under fluorescence microscopy using a Zeiss microscope.

Western blot analysis

Western blot analysis was performed as Cheng et al. [22]. HEI-OC1 cells were harvested, washed with ice cold 1×PBS, and resuspended in pre-cold 50 mM Tris-HCL buffer (pH 7.4) containing protease inhibitors (2 mM phenylmethanesulfonyl fluoride, 5 mM protease inhibitor cocktail, 1 mM Na orthovanadate). Protein concentration was measured at 595 nm using a SpectraMax M3 instrument. Sample (50 μg) were separated by 12% sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and were transferred to Polyvinylidene fluoride (PVDF) membranes. The membranes incubated in first antibody overnight and then incubated in secondary antibody at 4°C for 2 h. Proteins bands were detected using enhanced chemiluminescence system (Amersham Pharmacia Biotech, Piscataway, NJ, USA).

SIRT1 activity assay

HEI-OC1 cells were harvested, washed with ice-cold 1×PBS, and lysed with NETN buffer (1 mM EDTA, 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.5% NP-40). A SIRT1 fluorometric drug discovery kit (BML-AK511; Enzo Life Sciences, Plymouth Meeting, PA, USA) was used to detect SIRT1 activity. Briefly, Lysates incubated with SIRT1 assay buffer (45 min, 37°C), and then stopped by SIRT1 Developer (15 min, room temperature). At last, the sample was analysed by a Spectra M3 instrument at 355 nm/460 nm.

Comet assay

Cellular DNA damage was performed using a OxiSelect Comet Assay Kit according to the manufacturer’s protocol (Cell Biolabs, Inc, San Diego, CA, USA) as previously described [23]. Images were obtained and analyzed by 15 V. Komet 5.5 (Comet Assay Software; ANDOR technology, Belfast, Northern Ireland).

Statistical analysis

All data were repeated at least three independent times and were expressed as mean ± standard deviations. One-way analysis of variance and Student’s t-test were performed in these experiments. P < 0.05 was considered as statistically significant.

RESULTS

Luteolin inhibits H₂O₂-induced cellular senescence in HEI-OC1 cells

In this study, we evaluated the effect of luteolin on the viability of HEI-OC1 cells to determine its cytotoxic potential. Up to a concentration of 2 μM luteolin, no cytotoxic effects could be detected using the MTT assay (Fig. 1A). Piperine is a major plant alkaloid presented in pepper and was used as a positive control in present study. To investigate the effect of luteolin on the HEI-OC1 cells with senescent phenotype, cells were treated with 30 μM of H₂O₂ (Supplementary Fig. 1) for induction of cellular senescence. We found that pretreatment with luteolin inhibited the senescent phenotype as shown by the results of the SA-β-gal assay and appearance of enlarged cell morphologies at 3 days. After treatment with H₂O₂, 65% of the cells were found to be SA-β-gal-positive, which was reduced to only 17% in the presence of luteolin (Fig. 1B). The SA-β-gal-positive cells also exhibited senescent morphological changes such as enlarged and flattened shapes (Fig. 1C). Next, we monitored cell growth for 5 days under same condition. As expected, H₂O₂ significantly reduced the cell growth rate but the cells treated with luteolin are rescued more than 50% (Fig. 1D). In this setting, we did not find major differences on the portion of propidium iodide-stained cells, indicating that the reduced proliferation was not interfered by an increase in apoptosis (data not shown). These results indicated that luteolin effectively protected the HEI-OC1 cells against H₂O₂-induced cellular senescence.

Protective effect of luteolin against H₂O₂-induced cellular senescence is dependent on p53

As p53 and p21 are known to be major players in the induction of senescence [24], we investigated whether p53 played a role in mediating the protective effect of luteolin against H₂O₂-induced cellular senescence in HEI-OC1 cells. As shown in Fig. 2A, we observed that after H₂O₂ treatment, there was increased phosphorylation of p53 and expression of p21, both of which were decreased after luteolin treatment. Meanwhile, 80 nM p53 siRNA efficiently inhibited H₂O₂-induced p-p53 expression (Fig. 2B). We also found that blocking p53 using siRNA reversed luteolin effects on H₂O₂-treated cells as shown by the SA-β-gal activity assay results and senescent morphological changes on 3 days (Fig. 2C, D). These data suggested that luteolin protect cells from H₂O₂-induced cellular senescence via modulation of p53 phosphorylation and its downstream molecule, p21 expression.

Luteolin increases SIRT1 expression in HEI-OC1 cells

As shown in Fig. 1, luteolin protects against H₂O₂-induced cellular senescence and hence, we considered that luteolin might promote the expression of the longevity gene, SIRT1. HEI-OC1 cells were treated with various concentrations of luteolin (0–4 μM) for 12 h. The effect of luteolin on SIRT1 expression is shown in Fig. 3A and B. As shown in Fig. 3A, luteolin induced the expression of SIRT1 in a dose-dependent manner. The maximal induction of SIRT1 was achieved at a dose of 2 μM luteolin (Fig. 3A). The induction of SIRT1 expression by luteolin (2 μM) was detected as early as 3 h following luteolin treatment; the augmentation lasted for an additional 9 h (Fig. 3B). SIRT1 activity was found to increase over the same luteolin dose range (Fig. 3C) and to reach a maximum level after 12 h of luteolin treatment (Fig. 3D).

Inhibition of SIRT1 abrogates the protective effect of luteolin against H₂O₂-induced cellular senescence

To determine the role of endogenous SIRT1 in cellular senescence, HEI-OC1 cells were treated with SIRT1 siRNA. As shown in Fig. 4A and B, p-p53 and p21 expression were down-regulated by treatment of luteolin, while p-p53 and p21 expression were restored by silencing of SIRT1. In addition, SIRT1 inhibition abrogated the effect of luteolin on senescence-specific morphological changes and SA-β-gal activity. Under SIRT1 siRNA transfection, luteolin treatment did not increase the activity and expression of SIRT1 (Fig. 4C, D). These results demonstrated that luteolin inhibited oxidative stress-induced cellular senescence, which might be mediated by modulation of p53 and SIRT1.

Luteolin prevents H₂O₂-induced DNA damage in HEI-OC1 cells

There is a significantly increase in oxidative DNA damage in senescent cells, and the levels are similar to that seen in aging and age-related diseases [25,26]. Therefore, we examined whether luteolin has any protective role against the DNA damage caused by H₂O₂. We performed single cell gel electrophoresis assay, comet assay, to detect the DNA damage at individual cell level. As shown in Fig. 5A and B, the cells were treated with 2 μM luteolin for 12 h after exposure to 30 μM of H₂O₂ for 3 days show much shorter comet tails and smaller comet tail areas than the H₂O₂-only exposed cells (Fig. 5A). This significant difference in the tails between the groups is shown quantitatively in Fig. 5B, clearly indicating that luteolin markedly protects the cells against DNA damage under H₂O₂. To verify our hypothesis, we repeated this experiment in the SIRT1 or p53 silenced cells. As shown in Fig. 5A and B, SIRT1 siRNA efficiently abolishes the protective effect of luteolin on H₂O₂-induced DNA damage, whereas p53 siRNA did not show any significant changes. These results further underline the contribution of the accumulating DNA damage to cellular senescence and suggest that the SIRT1 plays a role in the prevention of DNA damage while p53 does not.

DISCUSSION

It has revealed that luteolin has protective roles in several disease models [11 -14]. However, as far as we know, the role of luteolin in aging is still unknown. Here, we showed that luteolin inhibits H₂O₂-induced cellular senescence and enhances SIRT1 expression concomitantly, which played a crucial role in inhibiting p53 as well as suppressing the senescent phenotypes in auditory cells (Fig. 6).

The anti-oxidant activity of luteolin has been associated with its ability to scavenge reactive oxygen species by inhibiting pro-oxidant enzymes and inducing anti-oxidant enzymes [27]. It has also been found to protect the microglia and alter hippocampal-dependent spatial working memory in aged mice [16,17]. As anticipated, luteolin prevented H₂O₂-induced cellular senescence as shown by attenuation of SA-β-gal signal and restring of cell growth (Fig. 1). In our observation, luteolin protects H₂O₂-induced cellular senescence by decreasing p53 phosphorylation (Fig. 2). Considering p53 is a key molecule that mediates multiple pathways involved in tumor suppression and senescence [28], several evidences that protective effect of Salidroside from premature senescence by regulation of redox and p53/p21 expression [29] and elevated level of p53 in H₂O₂ induced-prematurely senescent human diploid fibroblasts [30] raised us to investigate whether luteolin alters DNA damage under oxidative stress condition. Consistent with our expectation, luteolin ameliorated the H₂O₂-induced DNA damage (Fig. 5). As previous observation [29], luteolin downregulated p53 expression (Fig. 2).

SIRT1 plays an important role in protecting the cells against cellular oxidative stress and DNA damage [31]. Our results also showed that luteolin induces SIRT1 expression in HEI-OC1 cells and the upregulated SIRT1 expression prevented cellular senescence (Fig. 3). Previous studies have shown that SIRT1 overexpression has a protective role against oxidative stress-induced premature senescence in human endothelial cells [9] and reversely, SIRT1-deficient mouse embryonic fibroblasts extends replicative lifespan under sub-lethal oxidative stress [32]. Ota et al. [10] observed that senescence could be induced in WI38 and IMR90 cells using SIRT1 inhibitor. These effects could be reversed by inhibiting the transfection with SIRT1 siRNA. As shown in Fig. 4, SIRT1 siRNA efficiently abolishes the protective effect of luteolin on H₂O₂-induced DNA damage. Based on our data, luteolin influences SIRT expression (Fig. 5), which is indirectly implies its effect on DNA damage. Therefore, our observations along with ROS induced senescent pathway mediated by p53 are consistent with the concept that SIRT1 regulates important processes in cellular senescence. In addition, nicotinamide, a SIRT1 inhibitor, has been remarked as an important modulator to extend the replicative lifespan of primary human fibroblasts [33]. Taken together, SIRT1 has been considered as a key player to be able to make the resistance and modulate oxidative stress-induced premature senescence although still it is elusive whether it directly regulates lifespan. Interestingly, several reports have highlighted that dietary supplementation of flavonoids has a protective role against neurodegenerative, cardiovascular, inflammatory, metabolic diseases, and cancer by enhancing SIRT1 activity [34] and moreover, it has been reported that flavonoids, such as resveratrol, quercetin, and catechins, activate SIRT1 in vitro and in vivo [34 -37]. Therefore, it would be promising to regulate SIRT1 activity by dietary flavonoids as a therapeutic application against oxidative stress-induced cellular senescence.

A previous study proposed that SIRT1 might inhibit the ability of p53 to promote endothelial senescence [9]. In our study, we observed that the increase in the phosphorylation of p53 was higher in senescent cells compared to non-senescent cells, which was significantly decreased after treatment with luteolin (Fig. 2). This decrease was abolished by blocking SIRT1 expression using SIRT1 siRNA (Fig. 4), suggesting that the downregulation of p53 phosphorylation by luteolin is at least partially mediated by SIRT1. These findings are quite consistent with previous reports demonstrating that inhibition of SIRT1 by siRNA increased the acetylation of p53 in endothelial cells [9]. The SIRT1/p53 pathway in cellular proliferation and senescence is tightly linked with transcriptional alterations of downstream cell cycle inhibitors [32]. In the same manner, our data showed that regulation of p53 phosphorylation determines p21 expression level in HEI-OC1 cells (Figs. 2A, 4A).

Taken together, this study provides relevant evidences supporting that luteolin exerts protective effects against oxidative stress-induced cellular senescence which is derived by activation of p53. Subsequent enhancement of SIRT1 expression by luteolin plays a crucial role in inhibiting p53 activation as well as suppressing the senescent phenotype in auditory cells. These findings present luteolin as a potential and bio-safe natural compound to be able to impede aging process and attenuate age-related diseases incidence.

SUPPLEMENTARY MATERIALS

Supplementary data including one figure can be found with this article online at https://doi.org/10.4196/kjpp.2021.25.4.297.

kjpp-25-4-297-supple.pdf

ACKNOWLEDGEMENTS

This study was supported by Wonkwang University in 2020.

Notes

Author contributions: R.Z.Z., B.S.L., S.S.G., and B.M.C. conceived and designed the experiments. R.Z.Z., B.S.L., and S.S.G. performed experiments. B.S.L. and B.M.C analyzed data. J.H.S., and B.M.C. developed methodology and reviewed manuscript. B.S.L., J.H.S., and B.M.C. wrote the paper. B.M.C. supervised the study.

CONFLICTS OF INTEREST

The authors declare no conflicts of interest.

REFERENCES

1. Qian Y, Chen X. 2010; Tumor suppression by p53: making cells senescent. Histol Histopathol. 25:515–526. DOI: 10.14670/HH-25.515. PMID: 20183804. PMCID: PMC2841029.

2. Chen QM, Bartholomew JC, Campisi J, Acosta M, Reagan JD, Ames BN. 1998; Molecular analysis of H²O²-induced senescent-like growth arrest in normal human fibroblasts: p53 and Rb control G1 arrest but not cell replication. Biochem J. 332(Pt 1):43–50. DOI: 10.1042/bj3320043. PMID: 9576849. PMCID: PMC1219449.

3. Frippiat C, Dewelle J, Remacle J, Toussaint O. 2002; Signal transduction in H²O²-induced senescence-like phenotype in human diploid fibroblasts. Free Radic Biol Med. 33:1334–1346. DOI: 10.1016/S0891-5849(02)01044-4. PMID: 12419465.

4. MacNee W. 2005; Pulmonary and systemic oxidant/antioxidant imbalance in chronic obstructive pulmonary disease. Proc Am Thorac Soc. 2:50–60. DOI: 10.1513/pats.200411-056SF. PMID: 16113469.

5. Rahman I, Biswas SK, Kode A. 2006; Oxidant and antioxidant balance in the airways and airway diseases. Eur J Pharmacol. 533:222–239. DOI: 10.1016/j.ejphar.2005.12.087. PMID: 16500642.

6. Han J, Miyamae Y, Shigemori H, Isoda H. 2010; Neuroprotective effect of 3,5-di-O-caffeoylquinic acid on SH-SY5Y cells and senescence-accelerated-prone mice 8 through the up-regulation of phosphoglycerate kinase-1. Neuroscience. 169:1039–1045. DOI: 10.1016/j.neuroscience.2010.05.049. PMID: 20570715.

7. Luo J, Nikolaev AY, Imai S, Chen D, Su F, Shiloh A, Guarente L, Gu W. 2001; Negative control of p53 by Sir2alpha promotes cell survival under stress. Cell. 107:137–148. DOI: 10.1016/S0092-8674(01)00524-4. PMID: 11672522.

8. Ota H, Akishita M, Eto M, Iijima K, Kaneki M, Ouchi Y. 2007; SIRT1 modulates premature senescence-like phenotype in human endothelial cells. J Mol Cell Cardiol. 43:571–579. DOI: 10.1016/j.yjmcc.2007.08.008. PMID: 17916362.

9. Yi J, Luo J. 2010; SIRT1 and p53, effect on cancer, senescence and beyond. Biochim Biophys Acta. 1804:1684–1689. DOI: 10.1016/j.bbapap.2010.05.002. PMID: 20471503. PMCID: PMC2989880.

10. Ota H, Tokunaga E, Chang K, Hikasa M, Iijima K, Eto M, Kozaki K, Akishita M, Ouchi Y, Kaneki M. 2006; SIRT1 inhibitor, Sirtinol, induces senescence-like growth arrest with attenuated Ras-MAPK signaling in human cancer cells. Oncogene. 25:176–185. DOI: 10.1038/sj.onc.1209049. PMID: 16170353.

11. Ju W, Wang X, Shi H, Chen W, Belinsky SA, Lin Y. 2007; A critical role of luteolin-induced reactive oxygen species in blockage of tumor necrosis factor-activated nuclear factor-kappaB pathway and sensitization of apoptosis in lung cancer cells. Mol Pharmacol. 71:1381–1388. DOI: 10.1124/mol.106.032185. PMID: 17296806.

12. Shi RX, Ong CN, Shen HM. 2004; Luteolin sensitizes tumor necrosis factor-alpha-induced apoptosis in human tumor cells. Oncogene. 23:7712–7721. DOI: 10.1038/sj.onc.1208046. PMID: 15334063.

13. Xagorari A, Papapetropoulos A, Mauromatis A, Economou M, Fotsis T, Roussos C. 2001; Luteolin inhibits an endotoxin-stimulated phosphorylation cascade and proinflammatory cytokine production in macrophages. J Pharmacol Exp Ther. 296:181–187. PMID: 11123379.

14. Lin Y, Shi R, Wang X, Shen HM. 2008; Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets. 8:634–646. DOI: 10.2174/156800908786241050. PMID: 18991571. PMCID: PMC2615542.

15. Kang KP, Park SK, Kim DH, Sung MJ, Jung YJ, Lee AS, Lee JE, Ramkumar KM, Lee S, Park MH, Roh SG, Kim W. 2011; Luteolin ameliorates cisplatin-induced acute kidney injury in mice by regulation of p53-dependent renal tubular apoptosis. Nephrol Dial Transplant. 26:814–822. DOI: 10.1093/ndt/gfq528. PMID: 20817674.

16. Jang S, Dilger RN, Johnson RW. 2010; Luteolin inhibits microglia and alters hippocampal-dependent spatial working memory in aged mice. J Nutr. 140:1892–1898. DOI: 10.3945/jn.110.123273. PMID: 20685893. PMCID: PMC2937579.

17. Xu B, Li XX, He GR, Hu JJ, Mu X, Tian S, Du GH. 2010; Luteolin promotes long-term potentiation and improves cognitive functions in chronic cerebral hypoperfused rats. Eur J Pharmacol. 627:99–105. DOI: 10.1016/j.ejphar.2009.10.038. PMID: 19857483.

18. Kalinec GM, Webster P, Lim DJ, Kalinec F. 2003; A cochlear cell line as an in vitro system for drug ototoxicity screening. Audiol Neurootol. 8:177–189. DOI: 10.1159/000071059. PMID: 12811000.

19. Yu HB, Li DY, Zhang HF, Xue HZ, Pan CE, Zhao SH, Wang L. 2010; Resveratrol inhibits invasion and metastasis of hepatocellular carcinoma cells. J Anim Vet Adv. 9:3117–3124. DOI: 10.3923/javaa.2010.3117.3124.

20. Lee DS, Li B, Keo S, Kim KS, Jeong GS, Oh H, Kim YC. 2013; Inhibitory effect of 9-hydroxy-6,7-dimethoxydalbergiquinol from Dalbergia odorifera on the NF-κB-related neuroinflammatory response in lipopolysaccharide-stimulated mouse BV2 microglial cells is mediated by heme oxygenase-1. Int Immunopharmacol. 17:828–835. DOI: 10.1016/j.intimp.2013.08.024. PMID: 24055019.

21. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith O, Peacocke M, Campisi J. 1995; A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A. 92:9363–9367. DOI: 10.1073/pnas.92.20.9363. PMID: 7568133. PMCID: PMC40985.

22. Cheng XS, Li MS, Du J, Jiang QY, Wang L, Yan SY, Yu DM, Deng JB. 2011; Neuronal apoptosis in the developing cerebellum. Anat Histol Embryol. 40:21–27. DOI: 10.1111/j.1439-0264.2010.01033.x. PMID: 21231956.

23. Olive PL, Banáth JP. 2006; The comet assay: a method to measure DNA damage in individual cells. Nat Protoc. 1:23–29. DOI: 10.1038/nprot.2006.5. PMID: 17406208.

24. Muller M. 2009; Cellular senescence: molecular mechanisms, in vivo significance, and redox considerations. Antioxid Redox Signal. 11:59–98. DOI: 10.1089/ars.2008.2104. PMID: 18976161.

25. Andrade LN, Nathanson JL, Yeo GW, Menck CF, Muotri AR. 2012; Evidence for premature aging due to oxidative stress in iPSCs from Cockayne syndrome. Hum Mol Genet. 21:3825–3834. DOI: 10.1093/hmg/dds211. PMID: 22661500. PMCID: PMC3412382.

26. Tilstra JS, Robinson AR, Wang J, Gregg SQ, Clauson CL, Reay DP, Nasto LA, St Croix CM, Usas A, Vo N, Huard J, Clemens PR, Stolz DB, Guttridge DC, Watkins SC, Garinis GA, Wang Y, Niedernhofer LJ, Robbins PD. 2012; NF-κB inhibition delays DNA damage-induced senescence and aging in mice. J Clin Invest. 122:2601–2612. DOI: 10.1172/JCI45785. PMID: 22706308. PMCID: PMC3386805.

27. Wölfle U, Esser PR, Simon-Haarhaus B, Martin SF, Lademann J, Schempp CM. 2011; UVB-induced DNA damage, generation of reactive oxygen species, and inflammation are effectively attenuated by the flavonoid luteolin in vitro and in vivo. Free Radic Biol Med. 50:1081–1093. DOI: 10.1016/j.freeradbiomed.2011.01.027. PMID: 21281711.

28. Rodier F, Campisi J, Bhaumik D. 2007; Two faces of p53: aging and tumor suppression. Nucleic Acids Res. 35:7475–7484. DOI: 10.1093/nar/gkm744. PMID: 17942417. PMCID: PMC2190721.

29. Mao GX, Wang Y, Qiu Q, Deng HB, Yuan LG, Li RG, Song DQ, Li YY, Li DD, Wang Z. 2010; Salidroside protects human fibroblast cells from premature senescence induced by H²O² partly through modulating oxidative status. Mech Ageing Dev. 131:723–731. DOI: 10.1016/j.mad.2010.10.003. PMID: 21035481.

30. Duan J, Duan J, Zhang Z, Tong T. 2005; Irreversible cellular senescence induced by prolonged exposure to H²O² involves DNA-damage-and-repair genes and telomere shortening. Int J Biochem Cell Biol. 37:1407–1420. DOI: 10.1016/j.biocel.2005.01.010. PMID: 15833273.

31. Rajendran R, Garva R, Krstic-Demonacos M, Demonacos C. 2011; Sirtuins: molecular traffic lights in the crossroad of oxidative stress, chromatin remodeling, and transcription. J Biomed Biotechnol. 2011:368276. DOI: 10.1155/2011/368276. PMID: 21912480. PMCID: PMC3168296.

32. Chua KF, Mostoslavsky R, Lombard DB, Pang WW, Saito S, Franco S, Kaushal D, Cheng HL, Fischer MR, Stokes N, Murphy MM, Appella E, Alt FW. 2005; Mammalian SIRT1 limits replicative life span in response to chronic genotoxic stress. Cell Metab. 2:67–76. DOI: 10.1016/j.cmet.2005.06.007. PMID: 16054100.

33. Lim CS, Potts M, Helm RF. 2006; Nicotinamide extends the replicative life span of primary human cells. Mech Ageing Dev. 127:511–514. DOI: 10.1016/j.mad.2006.02.001. PMID: 16545428.

34. Chung S, Yao H, Caito S, Hwang JW, Arunachalam G, Rahman I. 2010; Regulation of SIRT1 in cellular functions: role of polyphenols. Arch Biochem Biophys. 501:79–90. DOI: 10.1016/j.abb.2010.05.003. PMID: 20450879. PMCID: PMC2930135.

35. Kaeberlein M, McDonagh T, Heltweg B, Hixon J, Westman EA, Caldwell SD, Napper A, Curtis R, DiStefano PS, Fields S, Bedalov A, Kennedy BK. 2005; Substrate-specific activation of sirtuins by resveratrol. J Biol Chem. 280:17038–17045. DOI: 10.1074/jbc.M500655200. PMID: 15684413.

36. de Boer VC, de Goffau MC, Arts IC, Hollman PC, Keijer J. 2006; SIRT1 stimulation by polyphenols is affected by their stability and metabolism. Mech Ageing Dev. 127:618–627. DOI: 10.1016/j.mad.2006.02.007. PMID: 16603228.

37. Davis JM, Murphy EA, Carmichael MD, Davis B. 2009; Quercetin increases brain and muscle mitochondrial biogenesis and exercise tolerance. Am J Physiol Regul Integr Comp Physiol. 296:R1071–R1077. DOI: 10.1152/ajpregu.90925.2008. PMID: 19211721.

Fig. 1

Luteolin reduces hydrogen peroxide (H₂O₂)-induced cellular senescence in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells.

(A–C) Cells were pretreated with luteolin for 12 h, then incubated in 30 μM H₂O₂ for 3 days. Cell viability was determined by MTT assay (A). The percentage of SA-β-gal positive cells out of total cells was counted and the average data was obtained from three independent experiments (B). The senescent phenotype of HEI-OC1 cells was detected by the SA-β-gal assay (×100) (C). (D) Cell growth was evaluated by MTT assay at various time points indicated in the figure after addition of H₂O₂. Data represents means values of triple experiments. *p < 0.05 vs. control, ^#p < 0.05 vs. H₂O₂. Piperine was used as a positive control.

Fig. 2

Protective effect of luteolin against hydrogen peroxide (H₂O₂)-induced cellular senescence is dependent on p53.

(A) Cells were pretreated with 2 μM luteolin for 12 h, then incubated in 30 μM H₂O₂ for 3 days. (B) Cells were transfected with various concentrations siRNAs of control or p53, then incubated with 30 μM H₂O₂ for 3 days. The protein levels were detected by Western blotting and quantified by densitometry based on immunoblot images (A, B). (C) Representative images of SA-β-gal staining of HEI-OC1 (×100). (D) The percentage of senescent cells were calculated in (D). Data represents means values of triple experiments. *p < 0.05 vs. control; ^#p < 0.05 vs. H₂O₂-treated cells.

Fig. 3

Effects of luteolin on sirtuin 1 (SIRT1) expression in House Ear Institute-Organ of Corti 1 cells (HEI-OC1).

(A, C) HEI-OC1 cells were treated with luteolin (0–4 μM, 12 h). (B, D) Cells were treated with luteolin (2 μM, 0–12 h). The proteins level (A, B) and SIRT1 activity (C, D) were performed with Western blotting and SIRT1 fluorometric kit, respectively. The proteins were quantified by densitometry based on immunoblot images. Data represents means values of triple experiments. Significance vs. control: *p < 0.05.

Fig. 4

Luteolin protects against hydrogen peroxide (H₂O₂)-induced p53 phosphorylation via sirtuin 1 (SIRT1) protein expression.

House Ear Institute-Organ of Corti 1 (HEI-OC1) cells were transfected with 80 nM siRNAs of control or SIRT1, then incubated in 2 μM luteolin for 12 h, followed by 30 μM H₂O₂ treatment for additional 3 days. (A, B) The protein levels were detected by Western blotting and quantified by densitometry based on immunoblot images. (C) Representative images of SA-β-gal staining of HEI-OC1 (×100). (D) The percentage of senescent cells were calculated in (D). Data represents means values of triple experiments. *p < 0.05 vs. control; ^#p < 0.05 vs. H₂O₂-treated cells.

Fig. 5

Luteolin prevents DNA from hydrogen peroxide (H₂O₂)-induced damage.

(A, B) House Ear Institute-Organ of Corti 1 (HEI-OC1) cells were transfected with 80 nM siRNAs of sirtuin 1 (SIRT1) or p53, then incubated in 2 μM luteolin for 12 h, followed by 30 μM H₂O₂ treatment for additional 3 days. Comet assay was performed as described in Methods. Data show the results of one of three independent experiments. *p < 0.05 vs. H₂O₂, ^#p < 0.05 vs. luteolin/H₂O₂/control siRNA.

Fig. 6

A schematic illustration summarizing the protective effect of Luteolin against hydrogen peroxide (H₂O₂)-induced premature senescence in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells.

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