Effects of Lactobacillus pentosus in Children with Allergen-Sensitized Atopic Dermatitis

So Hyun Ahn; Wonsuck Yoon; So Young Lee; Hee Soon Shin; Mi Young Lim; Young-Do Nam; Young Yoo

doi:10.3346/jkms.2020.35.e128

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Ahn, Yoon, Lee, Shin, Lim, Nam, and Yoo: Effects of Lactobacillus pentosus in Children with Allergen-Sensitized Atopic Dermatitis

Original Article

Pediatrics

Journal of Korean Medical Science 2020; 35(18): e128.

Published online: 19 March 2020

DOI: https://doi.org/10.3346/jkms.2020.35.e128

Effects of Lactobacillus pentosus in Children with Allergen-Sensitized Atopic Dermatitis

So Hyun Ahn¹

, Wonsuck Yoon²

, So Young Lee^3,⁴

, Hee Soon Shin^3,⁴

, Mi Young Lim³

, Young-Do Nam³

, Young Yoo^1,²

¹Department of Pediatrics, Korea University College of Medicine, Seoul, Korea.

²Allergy Immunology Center, Korea University, Seoul, Korea.

³Research Division of Food Functionality, Korea Food Research Institute, Wanju, Korea.

⁴KFRI School, Korea University of Science and Technology, Wanju, Korea.

Address for Correspondence: Young Yoo, MD, PhD. Department of Pediatrics, Korea University Anam Hospital, 73 Goryeodae-ro, Seongbuk-gu, Seoul 02841, Korea. yoolina@korea.ac.kr

Received 24 September 2019 Accepted 5 March 2020

The Korean Academy of Medical Sciences

(open-access, https://creativecommons.org/licenses/by-nc/4.0/):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Recent studies have shown that oral administration of probiotics may improve the immune imbalance caused by dysbiosis of the gut microbiome in atopic dermatitis (AD). This study aimed to investigate the clinical and immunological effects of Lactobacillus pentosus in children with mild to moderate AD.

Methods

Children aged 2–13 years with AD were randomized to receive either 1.0 × 10¹⁰ colony-forming units of L. pentosus or placebo, daily, for 12 weeks. The clinical severity of AD and transepidermal water loss were evaluated. Blood eosinophil counts, serum total immunoglobulin E (IgE), and cytokine levels were measured. The diversity and composition of the gut microbiota were also analyzed.

Results

Eighty-two children were recruited, and 41 were assigned to the probiotics intervention group. The mean scoring of atopic dermatitis (SCORAD) indices at baseline were 30.4 and 34.3 for the probiotics and placebo groups, respectively. At week 12, the mean indices were 23.6 and 23.1 for the probiotics and placebo groups, respectively. Clinical severity decreased significantly over time in both groups, with no significant difference between the two groups. In both groups, there were no significant differences in cytokine levels, microbial diversity, or the relative abundance of the gut microbiota at week 12 compared with the corresponding baseline values. The mean subjective scores of SCORAD indices after intervention for the probiotics group were significantly lower than those for the placebo group in IgE sensitized AD (P = 0.019).

Conclusion

Our results show improved symptoms in the probiotics and placebo groups, and we could not find additional effects of L. pentosus in AD. However, the mean subjective scores of SCORAD indices for the probiotics group are significantly improved compared with those for the placebo group in allergen-sensitized AD.

Graphical Abstract

Keywords: Atopic Dermatitis, Probiotics, Lactobacillus pentosus, Microbiome

INTRODUCTION

Atopic dermatitis (AD) is a chronic, recurrent, inflammatory skin disease characterized by extreme itching and dryness,1 affecting 10%–20% of children worldwide.2 This disease exhibits chronic progression from childhood and has been recognized as a refractory disease that is difficult to treat despite long-term therapy. Complex associations between genetic, environmental, and immunological factors induce the development of AD,3 and cellular immune dysregulation in AD results in a Th cell (Th1/Th2) imbalance, shifted toward Th2 cell differentiation. Given the complexity of the immune pathways that lead to AD, selective anti-inflammatory or immunomodulatory agents may be less effective. Thus, it is important to restore balance in immune pathways through fundamental immune regulation.

An imbalance of the gut microbiome may be associated with AD.4 Low microbial diversity in the gut microbiota in the first month of life has been associated with subsequent incidence of AD.5 6 Several studies have attempted oral administration of probiotics to alter gut microbiome imbalances, eventually restoring the immune balance and improving the symptoms of AD.7 Some studies have shown that probiotics are effective in controlling AD, but others have concluded that probiotics administration does not improve AD symptoms.

Lactobacillus pentosus derived from Kimchi is an effective modulator of the immune response.8 In house dust mite-sensitized AD mice, it increases the induction of regulatory B10 cells to produce IL-10.9 Lactobacillus plantarum, of the same genus as L. pentosus, was also shown to be effective in the treatment of AD in children.10

In this study, we investigated the clinical and immunological effects of L. pentosus in children with mild to moderate AD and aimed to observe the microbial diversity and composition of the gut microbiota. Additionally, we investigated whether probiotics administration is effective in subgroups with specific characteristics.

METHODS

Study subjects

One hundred and twenty-four subjects with mild to moderate AD (2–13 years old) were recruited from the Department of Pediatrics, Korea University Anam Hospital between January and September 2017. All subjects were diagnosed with AD by a pediatric allergist, according to the diagnostic criteria developed by Hanifin and Rajka,11 and the clinical severity was determined using the scoring of atopic dermatitis (SCORAD) index.12 Subjects with mild to moderate AD (SCORAD 20–50) were included. The exclusion criteria were as follows: 1) having additional systemic diseases, such as skin disease, immunodeficiency, or autoimmune disease; 2) treatment with systemic antibiotics, corticosteroids, antifungal agents, or immunosuppressants associated with AD within 4 weeks of screening; 3) treatment with medicines such as antihistamines, herbal medicines, or health functional foods for AD within 4 weeks of screening; 4) treatment with phototherapy within 4 weeks of screening; and 5) intent to participate in other studies during this clinical study.

Study design

This study was designed as a double-blinded, placebo-controlled, randomized study. Subjects were randomized to receive either live probiotics, specifically L. pentosus (1.0×10¹⁰ colony-forming units), or placebo twice a day for 12 weeks (Fig. 1). The probiotics and placebos were identical in color, taste, smell, packing, and manner of administration. All formulations were dispensed by a technician, with the investigator and subjects blinded to the identity of the intervention. Compliance was assessed from the weight of residual powder.

Fig. 1

Double-blinded, placebo-controlled, randomized study design.

L. pentosus = Lactobacillus pentosus, SCORAD = scoring of atopic dermatitis, TEWL = transepidermal water loss, IgE = immunoglobulin E.

Throughout the intervention period, the subjects were strictly prohibited from using corticosteroids, antibiotics, antihistamines, and other probiotics; only topical fluticasone propionate was allowed for severe flare-ups or itching. All subjects received an equal amount of skin emollients, and the weight of the tube containing topical steroids was quantitatively measured at each visit.

Assessment of clinical severity of AD

The primary outcome was the change in severity of AD between baseline and week 12. The clinical severity of AD was evaluated at consecutive visits (by the SCORAD index). The SCORAD index combines objective and subjective scores, including the evaluation of pruritus and insomnia on a visual analog scale. The range of the SCORAD index is between 0 and 80. Subjects were divided into three groups: mild (< 25), moderate (25–50), or severe (> 50). Subjects with a SCORAD index of < 20 or > 50 were excluded from this study.

Transepidermal water loss (TEWL) was assessed noninvasively with a Tewameter^® TM 300 (Courage+Khazaka, Köln, Germany) under standardized environmental conditions (indoor temperature of 22°C–26°C, relative humidity of 40%–60%, a ≥ 10-minute adaptation period, and exposed area of skin with clothes uncovered) using a standardized protocol,13 at baseline and at weeks 6 and 12. TEWL was measured on both forearms. The secondary outcome was the amount of topical steroid used, assessed by weighing the tubes.

Blood eosinophil counts and serum immunoglobulin E (IgE) levels

Blood samples were collected at baseline and at week 12. The number of peripheral blood eosinophils was measured using a Sysmex XE-2100 automated hematological analyzer (Sysmex, Kobe, Japan). The levels of total serum IgE and specific IgE antibodies against food allergens (egg white and soybean) and inhalant allergens (Dermatophagoides farinae) were determined using a fluorescence enzyme immunoassay (UniCAP, Phadia AB, Uppsala, Sweden). IgE sensitized AD was defined as blood specific IgE levels of > 0.7 kU/L.

Measurement of cytokine levels

Serum levels of IL-4, IL-5, IL-10, IL-13, and IFN-γ were measured using ELISA kits (R&D Systems, Minneapolis, MN, USA), whereas serum levels of IL-6, IL-17, and TGF-β were measured using a multiplex immunoassay based on Luminex technology (R&D Systems). The detection limits for IL-4, IL-5, IL-10, IL-13, and IFN-γ were 0.25, 3.9, 0.78, 125, and 15.6 pg/mL, respectively, and the maximum detection levels were 16, 150, 50, 4,000, and 1,000 pg/mL, respectively. The detection limits/maximum detection levels for IL-6, IL-17, and TGF-β were 0.95/2.53 pg/mL, 33.3/3,900 pg/mL, and 10,370/24,300 pg/mL, respectively.

Analysis of gut microbiome

specimen collection

Fecal samples from the subjects were collected in OMNIgene-GUT tubes (DNA Genotek, Ontario, Canada) as per the manufacturer's instructions and were stored at ambient temperature until they were processed.

DNA extraction

Metagenomic DNA was extracted from the fecal samples using the QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) with additional pre-processing steps. Briefly, the fecal samples (250 μL) stored in the OMNIgene-GUT tubes were transferred into 2 mL tubes containing 0.3 g of sterile 0.1 mm zirconia beads and 1.2 mL lysis buffer. After homogenization via vortexing, the samples were heated at 95°C for 15 minutes. Using a Qiagen TissueLyser II (Qiagen), the samples were bead beaten for 1 minute at 30 Hz, left on ice for 5 minutes, and then bead beaten for an additional minute. After centrifugation, the supernatant solution (1.2 mL) was reacted with an InhibitEX tablet and then centrifuged, after which 350 μL of the supernatant was transferred to a new tube. The subsequent DNA extraction steps were performed using a QIAcube (Qiagen). The extracted DNA samples were stored at −20°C until use.

16S rRNA gene sequencing and sequence analysis

For gut microbiome analysis, 16S rRNA gene sequencing was performed for the fecal samples at baseline and at weeks 12 and 14. Amplicon library preparation targeting the V3-V4 region of the 16S rRNA gene was performed according to the Illumina protocol for 16S Metagenomic Sequencing Library Preparation (Part # 15044223 Rev. B, Illumina, San Diego, CA, USA). Sequencing was performed on the MiSeq platform using the 2 × 300-bp MiSeq Reagent Kit v3. Raw sequence reads were processed for quality filtering and merging of read pairs, and subsequent analysis steps were performed using QIIME version 1.9.0 software.14 The sequences were clustered into operational taxonomic units (OTUs) based on 97% identity with pick_open_reference_otus.py. The α-diversity (observed species, Chao 1, phylogenetic diversity) and β-diversity (Bray-Curtis, weighted UniFrac, unweighted UniFrac distances) were calculated using core_diversity_analyses.py.

Statistical analysis

Comparison of mean values for continuous variables between the two groups was performed using t-tests, and the outcome values are represented as means ± standard deviations (SDs). Blood eosinophil counts and total serum IgE levels did not follow normal distributions, and therefore, are represented as natural log values of the means ± SDs. Comparison of frequencies between the two groups was performed using χ² tests. The analysis method for efficacy assessment variables was performed using analysis of covariance, which takes baseline characteristics (covariates, variables that are observed prior to probiotics administration that may affect primary efficacy assessment variables) into consideration. Statistical analysis was performed using IBM SPSS Statistics version 22.0 (IBM Corp., Armonk, NY, USA), and outcomes with a P value of < 0.05 were considered statistically significant.

Statistical analysis of bacterial abundance

Statistical analysis of bacterial abundance at each visit and in each group was performed at the genus level for microbes detected in ≥ 40% of the samples. Comparison of bacterial abundances at different visits (baseline vs. week 12; baseline vs. week 14; and week 12 vs. 14) within each group (probiotics vs. placebo) was performed using the Wilcoxon signed rank test. The log₂ fold change was obtained by adding 0.00001 to the bacterial relative abundance of each sample and calculating the log₂ ratio at week 12 or week 14 against the baseline. The difference in fold change between the probiotics and placebo groups was analyzed using the Wilcoxon rank sum test.

Ethics statement

Written informed consent was obtained from the parents of each subject. This study protocol was approved by the Institutional Review Board (IRB) of the Korea University Anam Hospital (IRB No. 2016-0371).

RESULTS

Subject characteristics

One hundred and twenty-four AD subjects were screened for eligibility. The following 29 subjects were excluded: 5 subjects with severe AD, 19 with loss of symptoms prior to probiotics administration, 3 with recent use of topical steroids, and 2 that were lost to follow-up. The remaining 95 subjects were randomly assigned and equally distributed between the two groups. However, there were 13 additional drop-outs who withdrew from the study; these included those who took antibiotics, discontinued the visits, or could not complete the study due to violation of study protocol. The remaining 82 subjects were finally recruited, resulting in 41 subjects in each group (Fig. 2). The proportion of male subjects was higher in the probiotics group than in the placebo group (P = 0.004). The mean age was 4.8 ± 2.3 years in the probiotics group and 5.4 ± 3.0 years in the placebo group (P = 0.305). However, there were no other significant differences between the two groups in baseline demographics (Table 1).

Fig. 2

Flowchart of the study.

SCORAD = scoring of atopic dermatitis.

Table 1

Demographics of subjects

Variables		Probiotics (n = 41)	Placebo (n = 41)	P value
Age, yr		4.8 ± 2.3	5.4 ± 3.0	0.305
Sex, male		25 (61)	12 (29)	0.004
Duration of illness, mon		37.5 ± 21.5	46.6 ± 30.6	0.124
Cesarean section		10 (24)	14 (34)	0.332
Family history of allergy		33 (81)	30 (73)	0.432
Environmental tobacco smoke		17 (41)	19 (46)	0.656
Bathing ≥ 1/day		12 (29)	15 (37)	0.467
Soaping ≥ 1/day		7 (17)	11 (27)	0.143
TEWL, g/m²/hr		30.3 ± 17.0	27.7 ± 11.9	0.428
SCORAD		30.4 ± 8.6	34.3 ± 8.3	0.040
	Objective	17.1 ± 8.8	21.0 ± 8.0	0.074
	Subjective	13.27 ± 2.61	13.34 ± 2.56	0.898
Log eosinophils, /µL		5.7 ± 0.7	5.9 ± 0.6	0.275
Log total IgE, kU/L		4.7 ± 1.5	4.5 ± 1.2	0.150
Sensitization to allergens
	Egg white	4 (9.8)	2 (4.9)	0.466
	Soybean	11 (26.9)	6 (14.6)	0.172
	Dermatophagoides farinae	17 (43.9)	18 (43.9)	0.857

Data are expressed as mean ± standard deviation or number (%).

TEWL = transepidermal water loss, SCORAD = scoring of atopic dermatitis, IgE = immunoglobulin E.

Changes in the clinical severity of AD

The mean SCORAD indices at baseline were 30.4 ± 8.6 for the probiotics group and 34.3 ± 8.3 for the placebo group. The mean SCORAD indices were significantly lower in the probiotics group than in the placebo group (P = 0.040). We also analyzed the mean objective and subjective scores of SCORAD indices separately. The mean objective and subjective scores of SCORAD indices were not significantly different between the probiotics and placebo groups (P = 0.074 and P = 0.898) (Table 1).

Comparing the mean SCORAD indices at baseline to those at week 12, the probiotics group exhibited a reduction from 30.4 ± 8.6 to 23.6 ± 11.0 (P < 0.001), and the placebo group exhibited a reduction from 34.3 ± 8.3 to 23.1 ± 9.7 (P < 0.001). The mean objective and subjective scores of SCORAD indices were not significantly different between the probiotics and placebo groups (P = 0.328 and P = 0.869) (Table 2).

Table 2

Clinical parameters in the two groups at baseline and at week 12

Variables		Probiotics (n = 41)			Placebo (n = 41)			P value^a
Variables		Baseline	Week 12	P value	Baseline	Week 12	P value	P value^a
SCORAD		30.4 ± 8.6	23.6 ± 11.0	< 0.001	34.3 ± 8.3	23.1 ± 9.7	< 0.001	0.254
	Objective	17.1 ± 8.8	12.3 ± 9.9	< 0.001	21.0 ± 8.0	11.8 ± 8.2	< 0.001	0.328
	Subjective	13.27 ± 2.61	11.32 ± 3.67	< 0.001	13.34 ± 2.56	11.24 ± 3.56	< 0.001	0.869
TEWL, g/m²/hr		30.3 ± 17.0	28.1 ± 11.3	0.444	27.7 ± 11.9	25.1 ± 9.1	0.265	0.233
Log total IgE, kU/L		4.7 ± 1.5	4.9 ± 1.5	0.122	4.5 ± 2.0	4.7 ± 1.2	0.044	0.563
Log eosinophils, /µL		5.7 ± 0.7	5.8 ± 0.8	0.241	5.9 ± 0.6	5.8 ± 0.7	0.777	0.470

Data are expressed as means ± standard deviation.

TEWL = transepidermal water loss, SCORAD = scoring of atopic dermatitis, IgE = immunoglobulin E.

^aCompared between groups; P value by analysis of covariance adjusted by baseline.

Both the probiotics and placebo groups exhibited significant improvements in the severity of AD, but no additional effect was observed for the probiotics group compared with the placebo group. The mean TEWL values at baseline were 30.3 ± 17.0 g/m²/hr for the probiotics group and 27.7 ± 11.9 g/m²/hr for the placebo group (P = 0.428) (Table 1). Similarly, the mean TEWL values at week 12 were 28.1 ± 11.3 g/m²/hr for the probiotics group and 25.1 ± 9.1 g/m²/hr for the placebo group (P = 0.233) (Table 2).

Changes in blood eosinophil counts, total serum IgE, and specific IgE

At baseline, there was no significant difference in the logarithmic mean concentration of total IgE between the two groups, with values of 4.7 ± 1.5 IU/mL in the probiotics group and 4.5 ± 2.0 IU/mL in the placebo group (P = 0.150). Similarly, the logarithmic mean blood eosinophil count at baseline was not significantly different, with 5.7 ± 0.7 cells/μL in the probiotics group and 5.9 ± 0.6 cells/μL in the placebo group (P = 0.275) (Table 1). The same pattern was observed at week 12; the logarithmic mean concentration of total IgE was 4.9 ± 1.5 IU/mL in the probiotics group and 4.7 ± 1.2 IU/mL in the placebo group (P = 0.563). The logarithmic mean blood eosinophil count was 5.8 ± 0.8/µL in the probiotics group and 5.8 ± 0.7/µL in the placebo group (P = 0.470) (Table 2). Among the 41 subjects in each group, 23 (56%) from the probiotics group and 26 (63%) from the placebo group were positive for specific IgE.

Changes in serum cytokine levels

The serum levels of IL-4, IL-5, IL-6, IL-10, IL-13, IL-17, IFN-γ, and TGF-β were measured at baseline. There were no significant differences between the two groups (Table 3). In addition, the cytokine levels at week 12 compared with the baseline within each group were not statistically significant (Table 4).

Table 3

Baseline cytokine levels in the two groups

Cytokine, pg/mL	Probiotics (n = 41)	Placebo (n = 41)	P value
IL-4	0.29 ± 0.26	0.26 ± 0.07	0.479
IL-5	3.90 ± 0.00	3.90 ± 0.00	1.000
IL-6	0.66 ± 0.92	0.61 ± 1.70	0.863
IL-10	0.95 ± 0.39	1.45 ± 2.23	0.810
IL-13	129.00 ± 24.60	125.20 ± 1.40	0.336
IL-17	0.43 ± 1.35	0.27 ± 0.76	0.505
IFN-γ	16.30 ± 4.20	15.60 ± 0.00	0.279
TGF-β	166,434 ± 36,403	179,179 ± 36,227	0.118

Data are expressed as means ± standard deviation.

IL = interleukin, IFN-γ = interferon-gamma, TGF-β = transforming growth factor-beta.

Table 4

Cytokine levels in the two groups at baseline and at week 12

Cytokine, pg/mL	Probiotics (n = 41)		Placebo (n = 41)		P value
Cytokine, pg/mL	Baseline	Week 12	Baseline	Week 12	P value
IL-4	0.29 ± 0.26	0.25 ± 0.00	0.26 ± 0.07	0.25 ± 0.00	-
IL-5	3.90 ± 0.00	4.24 ± 1.19	3.90 ± 0.00	3.90 ± 0.00	0.075
IL-6	0.66 ± 0.92	0.84 ± 1.65	0.61 ± 1.70	0.72 ± 0.92	0.740
IL-10	0.95 ± 0.39	1.24 ± 1.62	1.45 ± 2.23	1.59 ± 2.21	0.369
IL-13	128.96 ± 24.57	138.86 ± 71.34	125.22 ± 1.40	125.55 ± 3.52	0.303
IL-17	0.43 ± 1.35	0.49 ± 0.75	0.27 ± 0.76	0.38 ± 0.96	0.600
IFN-γ	16.32 ± 4.18	18.58 ± 12.24	15.60 ± 0.00	15.88 ± 1.29	0.366
TGF-β	166,434 ± 36,403	153,283 ± 41,393	179,179 ± 36,227	159,816 ± 34,250	0.750

Data are expressed as means ± standard deviation.

IL = interleukin, IFN-γ = interferon-gamma, TGF-β = transforming growth factor-beta.

Compared between groups; P value by analysis of covariance adjusted by baseline.

Gut microbiome analysis

Diversity of gut microbiota

The gut microbiomes of 76 subjects whose 16S sequence data were available for all the fecal samples at baseline and at weeks 12 and 14 were analyzed. First, the microbial α-diversity measured by the Chao 1 index was analyzed. No significant difference was observed in the Chao 1 index of the probiotics group compared with that of the placebo group at each visit (Fig. 3A). Next, the Bray-Curtis distances between different visits (baseline vs. week 12; baseline vs. week 14; and week 12 vs. 14) within each group (probiotics group vs. placebo group), respectively, were calculated. However, there was no significant difference in β-diversity, indicating similar degrees of overall compositional change between the probiotics and placebo groups (Fig. 3B).

Fig. 3

Changes in gut microbial diversity in the two groups. (A) The α-diversity, measured by Chao 1 index. (B) The β-diversity, measured by Bray-Curtis distance. Each bar indicates the distance between different visits (baseline vs. week 12, baseline vs. week 14, and week 12 vs. week 14) within each group (probiotics vs. placebo).

Microbiota characteristics

The relative abundance of the dominant taxa was analyzed at the phylum and genus levels (Fig. 4). At the phylum level, Bacteroidetes, Firmicutes, Proteobacteria, Actinobacteria, Verrucomicrobia, Fusobacteria, Cyanobacteria, and Tenericutes were detected in both the probiotics and placebo groups at baseline. Bacteroidetes was the most dominant microbe with an abundance of 45%–52%, followed by Firmicutes (36%–40%). At baseline, there was no statistically significant difference between the two groups at the phylum level (Fig. 4A). At the genus level, Bacteroides, Faecalibacterium, Prevotella, unclassified Ruminococcaceae, unclassified Clostridiales, and Sutterella were detected in both the probiotics and placebo groups at baseline, with Bacteroides being the most dominant microbe at 45%–46% abundance. Throughout the 12-week period, the relative abundance of Faecalibacterium and Prevotella (at the genus level) increased in both the probiotics and placebo groups, while that of unclassified Ruminococcaceae, unclassified Clostridiales, Sutterella, and unclassified Lachnospiraceae decreased in both groups (Fig. 4B).

Fig. 4

Relative abundance of dominant bacteria in gut microbiota in the two groups. (A) Phyla and (B) Genera levels.

Use of topical steroids

A total of 42 subjects whose AD symptoms were not managed, including 18 (44%) from the probiotics group and 24 (59%) from the placebo group, were given equal amounts of topical steroids. After the intervention period, the number of subjects whose symptoms were not managed was 10 (24%) in the probiotics group and 19 (46%) in the placebo group.

The mean weight of fluticasone propionate remaining in the tubes was 1.66 ± 3.29 g in the probiotics group and 2.99 ± 4.13 g in the placebo group at baseline (P = 0.111). When comparing the total weight of steroids used, there was no significant difference between the two groups (7.23 ± 6.22 g vs. 9.84 ± 9.37 g, respectively; P = 0.314) (data not shown).

Subgroup: allergic sensitization, age, and AD severity

Stratification of subjects by allergic sensitization, age, and AD severity indicated differences in changes in the SCORAD indices (Table 5).

Table 5

Changes in SCORAD indices of the probiotics and placebo groups with subgroup for allergic sensitization, age, and AD severity

Subgroups			Probiotics	Placebo	P value
IgE
	Sensitized (n = 43)
		Objective	5.6 ± 9.8	3.8 ± 8.0	0.724
		Subjective	2.7 ± 3.6	0.9 ± 3.0	0.019
	Non-sensitized (n = 39)
		Objective	7.8 ± 6.8	10.4 ± 0.9	0.148
		Subjective	0.9 ± 3.0	3.4 ± 3.3	0.013
Age
	≤ 6 yr (n = 59)
		Objective	6.1 ± 9.3	8.0 ± 8.5	0.752
		Subjective	2.7 ± 3.7	1.6 ± 2.9	0.131
	> 6 yr (n = 23)
		Objective	2.2 ± 10.4	7.8 ± 9.0	0.288
		Subjective	1.4 ± 3.5	2.4 ± 3.1	0.659
AD severity
	Mild (n = 26)
		Objective	2.7 ± 5.1	2.8 ± 5.4	0.910
		Subjective	1.6 ± 4.2	1.8 ± 2.7	0.989
	Moderate (n = 56)
		Objective	7.1 ± 11.4	9.3 ± 8.8	0.918
		Subjective	3.0 ± 3.2	1.9 ± 3.1	0.222

Data are expressed as means ± standard deviation.

SCORAD = scoring of atopic dermatitis, AD = atopic dermatitis, IgE = immunoglobulin E.

Compared between groups; P value by analysis of covariance adjusted by baseline.

In IgE sensitized AD, the mean SCORAD indices were 32.0 ± 9.4 and 35.5 ± 8.5 for the probiotics and placebo groups, respectively, at baseline (P = 0.222). However, the mean subjective scores of SCORAD indices at week 12 for the probiotics group were 10.4 ± 3.4, which was significantly lower than that at baseline (P = 0.001). Moreover, the mean subjective scores of SCORAD indices at week 12 for the probiotics group were significantly lower than that for the placebo group in IgE sensitized AD (P = 0.019) (Table 5). In contrast, it was lower in the placebo group than in the probiotics group in non-sensitized AD. The proportion of subjects who used topical steroids in the placebo group (64%) was approximately double than that in the probiotics group (29%), with the difference being significant (P = 0.034) (data not shown). However, there were no significant difference in changes in the SCORAD indices by age group between the probiotics and placebo groups. Similarly, the SCORAD indices by AD severity subgroups did not show any significant differences between the two groups (Table 5). There were no significant differences in α-diversity, β-diversity, and microbiota relative abundances between the probiotics and placebo groups among those with specific IgE sensitized AD (data not shown).

DISCUSSION

This was a double-blinded, placebo-controlled, randomized study to investigate the clinical and immunological effects of L. pentosus in children with mild to moderate AD. The following are the main findings: 1) clinical improvements were observed regardless of probiotics administration; 2) oral administration of L. pentosus did not alter serum cytokine levels; 3) the diversity of the gut microbiome was not significantly changed by probiotics administration; and 4) the mean subjective scores of SCORAD indices at week 12 for the probiotics group were significantly lower than those for the placebo group with IgE sensitized AD.

The neonatal immune system is shifted towards a Th2 response, and exposure to various common allergens after birth results in the maturation of Th1, Th2, Th17, and Treg cells.15 The gut microbiota plays an important role in mucosal immune system signaling, and gut dysbiosis alters immune regulation and precedes allergic diseases.16 17 Alteration of the gut microbiota by probiotics administration is expected to be an effective means of preventing AD from occurring. However, the therapeutic effects on AD have been inconclusive.18 19 20

L. pentosus, the main colonizer of the human intestines, is an anaerobic, non-sporulating, gram-positive bacterium extracted from fermented foods.21 22 L. pentosus functions in the production of mucosal IgA,23 and leads to increased levels of IL-10 and Foxp3+, which are involved in balancing the Th1/Th2 ratio, as well as suppressing IL-4.8 9 In house dust mite-sensitized AD mice, L. pentosus also reduced IL-4 and induced splenic regulatory B10 cells to produce IL-10.9

However, in this study, no statistically significant differences were observed in clinical severity, cytokine levels, microbial diversity, or the relative abundance of the gut microbiota between the probiotics and placebo groups. Factors that could have influenced these results are as follows. First, a single strain of L. pentosus was investigated in this study. In a previous study,7 a single strain showed clinical improvement in 7 out of 12 cases (58%), and mixed strains showed improvement in 8 out of 9 cases (89%). Previous meta-analyses on the effects of probiotics have also found that mixed strains improved the SCORAD index better than single strains.18 20 24 25 We studied a single strain in this analysis, but clinical severity decreased significantly over time in both groups, with no significant difference in the strength of this effect. Further research is needed to determine the synergistic effects of mixed strains of bacteria in modulating the gut microbiota and immune system. Second, the mean age in this study was older than that of patients in previous studies.26 27 The human intestinal microbiota evolves with age and stabilizes after 3 years of life.28 At birth, the gastrointestinal tract of the newborn is sterile, and the developing microflora in the early postnatal period is involved in the activation of innate and adaptive immunity.29 The infant microbiota structure is highly unstable and has low diversity compared to that of the adult.30 31 In this study, the mean age was over 3 years, and the duration of the disease was longer than that of studies conducted in infants. Thus, it is possible that the immune system is still under development in infants and that immunologic modulation by probiotics is less likely in children than in infants. Third, although a 12-week period is sufficient for assessing treatment efficacy, it may have been too short to induce alterations in the gut microbiota. A study on the prevention of AD showed a high diversity of the microbiota after 3 months of probiotics administration.32 A recent study identified an inverse relationship between SCORAD index and probiotics administration, indicating that probiotics administration influences the composition of the gut microbiota and functional changes.33

To the best of our knowledge, this is the first study to assess improvements in clinical severity and alterations in the gut microbiome in AD treatment in children. However, we found no significant difference in microbial diversity or relative abundance. Further investigation will be needed for longer periods of administration of L. pentosus.

Consistent with our results, many randomized clinical trials that have examined the effects of probiotics on AD did not find a positive effect in the entire study population.26 34 35 36 37 38 In several of these studies, however, the SCORAD index in IgE sensitized AD was significantly improved in the probiotics group.26 34 35 36 In our study, L. pentosus had a positive effect on the improvement of subjective symptoms only in IgE sensitized AD, and such improvement in IgE sensitized AD was lower in the placebo group than in the probiotics group. It is difficult to determine that the clinical significance was only based on the differences in the subjective scores of the SCORAD index between the two groups after probiotics administration. Further large-scale study is needed to find a clinical significance of probiotics in AD. These results suggest that the effects of probiotics may differ depending on the IgE sensitization of each subject. In the future, probiotics may be helpful in relieving symptoms when administered to subjects with IgE sensitized AD.

There are, however, several limitations to be considered. First, the potential effects of the additional intake of other fermented foods could not be excluded in this study. Although we provided instructions to minimize contamination from the intake of other probiotics, we could not completely prevent subjects from consuming fermented foods that contain various probiotics. In the future, more detailed instructions should be provided to reduce the dietary intake of probiotics. Second, the mean SCORAD indices at baseline were higher in the placebo group than in the probiotics group despite randomization; however, these were corrected for in the statistical analyses. Third, the mean subjective SCORAD indices were lower in the placebo group than in the probiotics group in non-sensitized AD. This is probably related to the difference in the proportion of subjects who used topical steroids and the amount of topical steroids used. In our study, the proportion of subjects who used topical steroids in the placebo group was higher than that in the probiotics group, with the difference being significant and the amount of topical steroids used for the placebo group being higher than that of the probiotics group in non-sensitized AD (data not shown). Fourth, in this study, allergen tests on two food allergens and one inhalant allergen were performed. The number of allergens was not enough to define allergen-sensitized AD. The results with subgroup for sensitization to each allergen were added, but there were no differences significantly (Supplementary Table 1). Finally, in the present study, we used the single dose of L. pentosus. It would have been better if our study was conducted to assess the dose-response relationship between our probiotics and AD severity. However, dose-response relationships of probiotics in terms of prevention or treatment of AD has not been reported yet. One animal study conducted with various doses of probiotics revealed that there is dose-response relationship between the probiotics and AD severity.39

In conclusion, subjective symptoms indicate that the degree of itching and the itch are caused by chemical mediators secreted by various inflammatory cells. Although there was no statistically significant change in cytokine levels in this study, the subjective symptoms of the IgE sensitized subgroup were improved. Therefore, it is believed that L. pentosus may be effective in allergen-sensitized AD and probiotics administration might be helpful in relieving symptoms in allergen-sensitized AD.

ACKNOWLEDGMENTS

The authors thank the field workers and other staff who supported in the data collection, and all the parents and children who participated in this study.

Notes

Funding: This study was supported by Main Research Program (E0170400-01) of the Korea Food Research Institute (KFRI) funded by the Ministry of Science and ICT, by Environmental Health Center for Childhood Asthma funded by the Ministry of Environment, Korea, by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Science, Korea (NRF-2018R1A6A3A11049961, NRF-2019R1F1A1041169).

Disclosure: The authors have no potential conflicts of interest to disclose.

Author Contributions:

Conceptualization: Yoo Y.
Data curation: Lee SY, Shin HS.
Formal analysis: Lee SY, Ahn SH, Lim MY, Nam YD.
Investigation: Ahn SH.
Writing - original draft: Ahn SH.
Writing - review & editing: Ahn SH, Yoon W, Yoo Y.

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SUPPLEMENTARY MATERIAL

Supplementary Table 1

Changes in SCORAD indices of the probiotics and placebo groups with subgroup for sensitization to each allergen

jkms-35-e128-s001.xls

TOOLS

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