Effects of Novel Probiotics in a Murine Model of Irritable Bowel Syndrome

Hye Gyo Chung; Yang Won Min; Chansu Lee; Sung Noh Hong; Ji Young Won; Jin Ah Jang; Cheol-Hyun Kim; Dong Kyung Chang

doi:10.4166/kjg.2020.75.3.141

Journal List > Korean J Gastroenterol > v.75(3) > 1144390

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Chung, Min, Lee, Hong, Won, Jang, Kim, and Chang: Effects of Novel Probiotics in a Murine Model of Irritable Bowel Syndrome

Original Article

Korean J Gastroenterol 2020;75(3):141-146.

Published online: 4 October 2020

DOI: https://doi.org/10.4166/kjg.2020.75.3.141

Effects of Novel Probiotics in a Murine Model of Irritable Bowel Syndrome

Hye Gyo Chung, Yang Won Min

, Chansu Lee, Sung Noh Hong, Ji Young Won¹, Jin Ah Jang¹, Cheol-Hyun Kim¹, Dong Kyung Chang

Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea

¹Department of Animal Resource & Science, Dankook University, Cheonan, Korea

Correspondence to: Yang Won Min, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 81 Irwon-ro, Gangnam-gu, Seoul 06351, Korea. Tel: +82-2-3410-3409, Fax: +82-2-3410-6983, E-mail: yangwon.min@samsung.com

Received 23 November 201928 December 2019 Accepted 8 January 2020

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background/Aims

Dysbiosis is an important factor in the pathogenesis of irritable bowel syndrome (IBS). Several studies have reported promising results using probiotics for the treatment of IBS. This study evaluated the efficacy of novel probiotics isolated from Kimchi, a Korean fermented food, and the feces of healthy Vietnamese people in a murine model of IBS.

Methods

Lactobacillus paracasei DK121 was isolated from Kimchi, and L. salivarius V4 and L. plantarum V7 were isolated from the feces of healthy Vietnamese people residing in Korea. Forty rats were allocated to receive one of the study strains, a mixture of the strains, or the vehicle. After 5 days of administration, the rats were restrained in a cage to induce IBS. The effects of the probiotics on IBS were analyzed by evaluating the stool weights and stool consistency scores.

Results

The primary outcome was analyzed upon the completion of a three-week experiment. The rats in the V7 group showed lower stool weights than those in the control group at week 2 (median: 1.10 [V7] vs. 2.35 [control], p=0.04, Mann-Whitney U-test) and week 3 (median: 1.10 [V7] vs. 2.80 [control], p=0.017). The rats in the DK121 (median: 2.00, p=0.007), V7 (median: 2.00, p=0.004), and mixture (median: 1.50, p=0.001) groups showed better stool consistency scores at week 2 than the control group (median: 3.00).

Conclusions

The novel probiotics have beneficial effects on defecation in a murine model of IBS. Human studies confirming the efficacy are warranted.

Keywords: Probiotics, Irritable bowel syndrome, Defecation

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Fig. 1.

Study design.

Fig. 2.

Cylindrical cage used to confine the rats during the experiment (diameter, 70 mm; height, 220 mm). Each rat was restrained in this cage for 1 hour on days 6, 13, and 20, provoking stress-induced defecation.

Fig. 3.

Body weights of rats during the 3-week study period. Each dot represents the median body weight (g) of the rats in the assigned group.

Fig. 4.

Stool weights of the rats in each study group during the 3 weeks of probiotics administration. The rats in the V7 group had lower stool weights than those in the control group at weeks 2 (p=0.040) and 3 (p=0.017). The rats in the DK121 group had lower stool weights than the control group at week 3 with marginal statistical significance (p=0.073). ^∗ The lines are the median and interquartile range of the eight animals in each group. Each value is shown as a scatter dot. Lines and scatter dots are shown for the control, DK121, V4, V7, and the mixture groups.

Fig. 5.

Stool consistency scores of the rats in each study group during the 3-week probiotics treatment experiment. The rats in the DK121(p=0.007), V7 (p=0.004), and the mixture (p=0.001) groups showed better (lower) stool consistency scores than the control group at week 2. No significant difference was observed among the study groups at week 3. ^∗ The lines represent the median and interquartile range of the eight animals in each group. Each value is shown as a scatter dot. The lines and scatter dots are shown for the control, DK121, V4, V7, and the mixture groups.

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ORCID iDs: Yang Won Min
https://orcid.org/0000-0001-7471-1305
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