Journal List > J Bacteriol Virol > v.50(1) > 1144270

Kang and Ham: Age-specific Prevalence of Porcine Reproductive and Respiratory Syndrome Virus, Porcine Circovirus Type 2, and Mycoplasma hyopneumoniae in Korea Pig Farms

Abstract

Porcine respiratory disease complex (PRDC) continues to be a significant economic problem to the swine industry. Porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and Mycoplasma hyopneumoniae (MH) are considered to be the most important pathogens that cause PRDC. In this study, we investigated the prevalence of antibodies against PRRSV and MH in the serum of sows and piglets from 89 domestic commercial pig farms by ELISA, and the presence of viral nucleic acids of PRRSV, including North American and European PRRS, and PCV2 was also investigated in the serum of sows and piglets from 89 domestic commercial pig farms by real-time PCR. In case of PRRSV, 78.7% (70/89) of sows were positive for PRRSV antibody, and 96.6% (86/89) of piglets were positive for PRRSV antibody. For MH, 76.4% (68/89) of sows showed positive for MH antibody. In the PRRSV viral nucleic acid detection experiment, 36.0% (32/89) of sows were positive for PRRSV nucleic acids, and virus nucleic acid was detected in 83.1% (74/89) of piglets. In case of virus type, both North American and European types were detected. In case of PCV2, 15.7% (14/89) of sows were positive for PCV2 nucleic acids. Conclusively, PCV2, PRRSV, and MH were widely distributed in pig farms in Korea. These prevalence data related with PRDC provides clinical information for vaccination strategy and development for the control of PRDC.

REFERENCES

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Table 1.
Primer sequences used by real time PCR for the survey of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in Korea
Pathogen Primers (‘5-3’) PCR production Reference‡
North American PRRSV forward: 5′ TGTGCCAGATGCTGGGTAAGATCA backward: 5′ ATTGACGACAGACACAATTGCCGC 172 base pairs Wasilk et al, 2004
European PRRSV forward: 5′ TGGCCAGTCAGTCAATCAAC backward: 5′ AATCGATTGCAAGCAGAGGGAA 603 base pairs Wasilk et al, 2004
PCV2 forward: 5′ GCACAGAGCGGGGGTTTG backward: 5′ ACCGCTGGAGAAGGAAAAAT 168 base pairs Gagnon et al., 2008

PRRSV, porcine reproductive and respiratory syndrome virus; PCV2, porcine circovirus type 2; PCR, polymerase chain reaction

Table 2.
Detection rate of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), and Mycoplasma hyopneumoniae (MH) antibodies and antigens in pigs during 2016–2018 in Korea
PRRSV pattern of sows PRRSV pattern of piglets PCV2 infection pattern MH antibody pattern
Antibody Antigen Type Farm number Antibody Antigen Type Farm number Sows Piglets Farm number Sows Piglets Farm number
North American European North American European
+ + + 6 + + + 27 + + 5 + + 46
+ + - 14 + + - 25 + - 9 + - 22
+ - + 11 + - + 22 - + 18 - + 15
+ - - 39 + - - 12 - - 57 - - 6
- + - 1 - + - 0            
- - - 18 - - - 3            
  Total   89   Total   89 To otal 89 To otal 89
Table 3.
Age distribution of porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), and Mycoplasma hyopneumoniae (MH) antibodies and antigens in piglets during 2016–2018 in Korea
Age (weeks) North American PRRSV positive European PRRSV positive PCV2 positive MH antibody positive
1 4 2 0 6
2 0 0 0 2
3 1 1 0 1
5 16 13 6 13
6 0 2 0 0
7 15 15 5 3
8 0 0 1 1
9 1 0 0 1
10 8 4 4 9
16 4 5 5 12
20 2 0 2 9
21 0 0 0 1
Total 51 42 23 58

Serum samples were taken and pooled from five pigs at each week of age (n=89).

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