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Lab Med Online. 2019 Oct;9(4):258-262. English.
Published online Oct 01, 2019.  https://doi.org/10.3343/lmo.2019.9.4.258
© 2019, Laboratory Medicine Online
Acute Monoblastic Leukemia with t(11;17)(q23;q21): Fusion of the KMT2A(MLL) and MLLT6(AF17) Genes
Cheon-Gang Park, M.D., Seon-Ho Mun, M.D., A-Jin Lee, M.D., Chang-Ho Jeon, M.D., Hun Suk Suh, M.D. and Sang-Gyung Kim, M.D.
Department of Laboratory Medicine, Daegu Catholic University School of Medicine, Daegu, Korea.

Corresponding author: Sang-Gyung Kim, M.D., Ph.D. Department of Laboratory Medicine, Daegu Catholic University Medical Center, 33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 42472, Korea. Tel: +82-53-650-4145, Fax: +82-53-653-8672, Email: sgkim@cu.ac.kr
Received Dec 11, 2018; Revised May 06, 2019; Accepted May 09, 2019.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Abstract

The KMT2A (formerly MLL) gene is associated with at least 10% of all cases of acute leukemia. More than 80 translocation partner genes of KMT2A have been discovered to date, six of which have been identified on the long arm of chromosome 17. Among these, the MLLT6 (formerly AF17) gene is located at 17q12 and fuses with the KMT2A gene in rare cases of acute leukemia. We report here a case of AML with a KMT2A/MLLT6 fusion that was confirmed using molecular genetic methods. According to a literature review, this is the first reported case of AML with a KMT2A/MLLT6 fusion in Korea.

Keywords: Acute monoblastic leukemia; AML; KMT2A; MLL; MLLT6

INTRODUCTION

The lysine K-specific methyltransferase 2A (KMT2A) gene, formerly known as the MLL gene, is located on chromosome 11q23.3 [1], and its chromosomal rearrangement is implicated in at least 10% of all acute leukemia (AL) cases of various types, including ALL, AML, biphenotypic AL, treatment-related leukemia, and infant leukemia [2]. The KMT2A protein acts as a transcriptional regulator in normal hematopoiesis; AL caused by KMT2A translocation tends to exhibit a more aggressive clinical course than that caused by other etiologies [3].

According to the French-American-British (FAB) classification of AML that is based on cytomorphology and cytochemistry [4], AL caused due to a KMT2A translocation is strongly associated with the monocytic lineage, including an association with M5a in 38.5% of cases, M5b or M4 in 21.2% of cases each, and M2 in 9.6% of cases [5].

More than 80 translocation partner genes (TPGs) of the KMT2A gene have been described to date, many of which have already been cloned and analyzed at the molecular level [2]. Six TPGs located on the long arm of chromosome 17 have been reported: KMT2A/GAS7 (17p13), KMT2A/ACACA (17q21), KMT2A/LASP1 (17q21), KMT2A/MLLT6 (17q21), KMT2A/RARA (17q21), and KMT2A/SEPT9 (17q25) [1].

MLLT6, a PHD finger containing (MLLT6) gene, formerly known as AF17, is located at 17q12, proximal to RARA, which has also been detected in 17q21 [6]. Fusion of the KMT2A and MLLT6 genes is quite rare in AML etiology, as only 2.85% (54 in 1,897) AML cases were confirmed to harbor KMT2A rearrangement [5], and 1.55% (9 in 579) cases of KMT2A-related AML showed a KMT2A/MLLT6 fusion [7]. Herein, we report a case of AML with the KMT2A/MLLT6 fusion resulting in a monoblastic morphology, representing the first such case in Korea, and provide context for the literature on AML showing a KMT2A/MLLT6 fusion reported to date.

CASE REPORT

A 69-year-old female with a history of hypertension and atherosclerosis after having a fever for one week, accompanied by headache, malaise, and epigastric pain, presented to our hospital. Physical examination showed an afebrile state, without hepatosplenomegaly or abdominal tenderness. A complete blood count revealed a leukocyte count of 6.3×109/L, hemoglobin at 9.6 g/dL, and the platelet count at 5.4×109/L. A peripheral blood smear and manual differential count showed 64% blasts and 26% monocytes. The bone marrow aspirate showed 86.9% large leukemic blasts of an FAB M5a morphology (no faggot cells, Fig. 1A and 1B). Thus, it was diagnosed to be AML. In cytochemistry, the cells were positive for Periodic acid–Schiff stain, and negative for myeloperoxidase, Sudan black B, and dual esterase (<3%). Bone marrow biopsy showed 90% cellularity, and immunohistochemistry showed focal positivity for myeloperoxidase and negativity for terminal deoxynucleotidyl transferase. Immunophenotyping was positive for CD45, CD34, HLA-DR, CD13, CD33, CD64, and CD117, while CD14 was negative.


Fig. 1
Peripheral blood and bone marrow smears of the patient. (A) A peripheral blood smear shows monoblasts with abundant cytoplasm and prominent nucleoli (Wright stain, ×400). (B) A bone marrow aspiration smear shows uniform proliferation of monoblasts which have round/folded nuclear and abundant cytoplasm (Wright stain, ×400).
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Chromosomal analysis showed t(11;17)(q23;q21) (Fig. 2), and the reverse transcription (RT)-PCR test (HemaVision-28N, DNA Diagnostics A/S, Risskov, Denmark) was positive for the KMT2A/MLLT6 fusion (Fig. 3). Treatment was initiated with cytarabine and daunorubicin. After one month of therapy, follow-up bone marrow aspiration showed a hypocellular marrow with 2.49% blasts. No chromosomal analysis was conducted at this point due to the lack of mitotic cells; however, FISH analysis (KMT2A Break-apart, Cytocell, Cambridge, UK) identified a rearrangement of the KMT2A gene in 2.5% of interphase cells (Fig. 4). No further chemotherapy was administered due to neutropenia. Four months after the initial diagnosis, the leukemia relapsed, and the patient ultimately died from septic shock. Next-generation sequencing (NGS) of the frozen RNA sample (taken at initial diagnosis) and a gene panel test which can identify the gene fusion (Oncomine Myeloid Research Assay, Thermo Scientific Inc., Waltham, MA, USA) were negative for the fusion gene.


Fig. 2
Chromosomal analysis showed the translocation, 46,XX,t(11;17)(q23;q21)[19]/46,XX[1] (trypsin-Giemsa G-band).
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Fig. 3
(A) A reverse transcription-PCR assay shows a band in the second lane (M2). (B) Split-out image shows a band (approximately 600 base pairs) in lane 2B, which indicates KMT2A/MLLT6 fusion transcripts.
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Fig. 4
FISH analysis of a follow-up bone marrow sample with dual color (green and red) probes of KMT2A. The circled cell shows one yellow, one green and one red signal, which indicate KMT2A rearrangement (in 5/200 interphase cells, reference range: < 2%).
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DISCUSSION

A total of 13 cases of AML with the KMT2A/MLLT6 fusion have been reported to date [2, 8, 9, 10, 11, 12, 13] (Table 1). Prasad et al. [8] reported the first such case involving the fusion of KMT2A exon 5 and an unknown exon of MLLT6 based on RT-PCR and the Sanger sequencing method. Suzukawa et al. [9] found a breakpoint at KMT2A exon 6 and MLLT6 exon 9, and confirmed the KMT2A rearrangement by FISH analysis. Moore et al. [10] used a gene-specific probe to confirm the KMT2A/MLLT6 fusion in FISH analysis, whereas Grossman et al. [12] applied NGS for identification of this fusion for the first time with the pyrosequencer (454 FLX, Roche Diagnostics, Rotkreuz, Switzerland).


Table 1
Reported cases* with confirmed KMT2A/MLLT6 fusions and their cytogenetic characteristics
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The MLLT6 gene is located on chromosome 17q12, but karyotype analysis has provided diverse results such as 17q21 [9–12 and present case] or 17q12-21 [2] in previous literature. This discrepancy is likely due to resolution differences in the karyotype analyses [10] or reflects the molecular heterogeneity of AML patients [11].

In our case, we used NGS sequence analysis (S5 XL, Thermo Scientific Inc.) and a gene panel test for hematologic malignancy (Oncomine Myeloid Research Assay, Thermo Scientific Inc.) to identify the gene fusion, but were unable to find the gene fusion using these methods. KMT2A is included in the RNA fusion gene list of the assay [14], but the sample was stored for about two years at −20℃. Since ribonuclease can remain active at this temperature [15], the low sample quality might explain this negative result.

This is the first case of KMT2A/MLLT6 fusion in a patient with AML reported in Korea. Kim et al. [16] reported a case of AML M5 with t(11;17)(q23;21) in 2006. In this case, FISH analysis revealed that the breakpoint in chromosome 17 was proximal to the RARA gene, but the authors did not perform a RT-PCR test to confirm the KMT2A gene rearrangement. Several cases of AML have been reported to be associated with rearrangement of the KMT2A gene with the region proximal to the RARA gene [17, 18]; however, these might not involve the KMT2A/MLLT6 fusion, because two other TPGs (besides MLLT6 and RARA) are located in that region [1] and should thus be confirmed by more sensitive assays such as RT-PCR or NGS.

Unlike the FAB classification based on morphological findings, the WHO classification uses cytogenetic characteristics for classification. AML with the KMT2A/MLLT6 fusion is not yet accepted as a full entity in the WHO classification [19], but the aggressive disease course shown in the present case emphasizes the importance of molecular genetic assays in addition to morphology, cytochemistry and cytogenetic analyses. King et al. [20] reported 12% (7/57) of cases of acute leukemia which had translocation were positive using the RT-PCR method and negative using the conventional cytogenetic assay. Therefore, for patients newly diagnosed as having AL, RT-PCR or FISH should be performed to find chromosomal aberrations not detected by conventional cytogenetic assays.

Notes

AUTHORS' DISCLOSURES OF POTENTIAL CONFLICTS OF INTEREST:No potential conflicts of interest relevant to this article were reported.

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