Journal List > J Korean Acad Oral Health > v.43(4) > 1140757

Park, Choi, Song, and Kim: Antifungal effects of hydrogen water on the growth of Candida albicans

Abstract

Objectives

This study assessed the antifungal effects of neutral electrolysis hydrogen water, an agent known for its oral anti-bacterial effects on the growth of C. albicans in vitro.

Methods

Hydrogen water, denture cleansing agent, and Listerine® were compared to a tap water control. We serially cultured C. albicans KCTC 27239 in SD media to analyze the inhibitory effect of each treatment on growth. We evaluated the data using repeated-measures ANOVA test and one-way ANOVA test, and a significant difference was defined as P<0.05.

Results

Hydrogen water significantly inhibited the growth, but not the biofilm formation, of C. albicans compared to tap water control at 1-, 5-, and 10-minute time points (P<0.05).

Conclusions

The growth of C. albicans was slower in hydrogen water than in tap water, thereby suggesting an inhibitory effect of hydrogen water. Hydrogen water may, therefore, be utilized as an antibacterial and preventive agent without any harmful effect on denture and oral tissues.

Figures and Tables

Fig. 1

Intervention growth curve of C. albicans. C. albicans growth curve of exponential growth phase.

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Fig. 2

Antifungal effect of the various rinsing water against C. albicans according to processing time. The data were analysed by one-way ANOVA test and denoted by Tukey's post-hoc analysis. *Indicates a significant difference compared with control (P<0.05).

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Fig. 3

Antifungal effect of the various rinsing water against C. albicans biofilm according to processing time. The data were analysed by one-way ANOVA test and denoted by Tukey's post-hoc analysis.

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TOOLS
ORCID iDs

Mi-Jung Park
https://orcid.org/0000-0002-1379-2057

Youn-Hee Choi
https://orcid.org/0000-0001-5712-8097

Keun-Bae Song
https://orcid.org/0000-0002-5416-5500

Eun-Kyong Kim
https://orcid.org/0000-0001-9582-1415

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