Abstract
Background
Methods
Results
Acknowledgments
Notes
Authors' Contributions:
Conceptualization: Ahn JJ, Kim BC.
Methodology: Ahn JJ, Ra SW.
Formal analysis: Ra SW, Kang BJ.
Data curation: Lim S.
Validation: Lee K, Kim BC.
Investigation: Son SH, Kang BJ.
Writing — original draft preparation: Kang BJ, Ra SW.
Writing — review and editing: Lee K, Kim BC, Lim S, Son SH, Ahn JJ
Approval of final manuscript: all authors.
References
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Figure 1
Four cases of primary tumor volume measurement using a high-performance workstation. The transverse (left upper, F), coronal (left lower, A), and sagittal (right lower, L) area of the tumor was examined using computed tomography attenuation values. Then, the device automatically reconstructed 3D images of the tumor (right upper, A).
![trd-83-61-g001](/upload/SynapseXML/0003trd/thumb/trd-83-61-g001.jpg)
Figure 2
Workflow of circulating tumor cell (CTC) enrichment and enumeration (A), and CTC analysis (B) by the BioView Duet Workstation. WBC: white blood cell; PBMC: peripheral blood mononuclear cell.
![trd-83-61-g002](/upload/SynapseXML/0003trd/thumb/trd-83-61-g002.jpg)
Figure 3
Schematic flow chart of study population selection. CTC: circulating tumor cell; FAST: fluid-assisted separation technology.
![trd-83-61-g003](/upload/SynapseXML/0003trd/thumb/trd-83-61-g003.jpg)
Figure 4
Receiver operating characteristics curve for the diagnostic performance of circulating tumor cell positivity for lung adenocarcinoma, assessed using the propensity score-matched cohort. AUC: area under the curve.
![trd-83-61-g004](/upload/SynapseXML/0003trd/thumb/trd-83-61-g004.jpg)
Figure 5
Linear regression model analysis for the association of circulating tumor cell (CTC) number with primary tumor volume. (A) All lung adenocarcinoma patients (n=21) (R2=0.542, β=0.736, p<0.001, CTC=−0.050+0.272×primary tumor volume). (B) Lung adenocarcinoma patients without distant metastasis (n=11) (R2=0.030, β=−0.172, p=0.612, CTC=0.496−0.091×primary tumor volume). (C) Lung adenocarcinoma patients with distant metastasis (n=10) (R2=0.491, β=0.701, p=0.024, CTC=−0.230+0.286×primary tumor volume). (D) Lung adenocarcinoma patients with extra-thoracic metastasis (n=6) (R2=0.719, β=0.848, p=0.033, CTC=−1.558+0.277×primary tumor volume).
![trd-83-61-g005](/upload/SynapseXML/0003trd/thumb/trd-83-61-g005.jpg)
Table 1
Baseline characteristics of lung adenocarcinoma patients with CTC-negative and CTC-positive
![trd-83-61-i001](/upload/SynapseXML/0003trd/thumb/trd-83-61-i001.jpg)
Table 2
Baseline characteristics of lung adenocarcinoma patients and healthy controls, before and after propensity matching
![trd-83-61-i002](/upload/SynapseXML/0003trd/thumb/trd-83-61-i002.jpg)
Values are presented as median (range) or number (%).
*Statistical comparisons of the data were performed using chi-square tests for categorical variables and Mann–Whitney U tests for continuous variables. †Statistical comparisons of the data were performed using the McNemar test for categorical variables and Wilcoxon signed-rank tests for continuous variables.
CTC: circulating tumor cell.
Table 3
Simple and multiple linear regression analysis of circulating tumor cell number related factors in patient with lung adenocarcinoma
![trd-83-61-i003](/upload/SynapseXML/0003trd/thumb/trd-83-61-i003.jpg)