Prevalence and Species Spectrum of Pulmonary Nontuberculous Mycobacteria Isolates at a Tertiary Care Center

Young Sun Joo; Na Eun Kwak; Gun Han Kim; Eun-Jeong Yoon; Seok Hoon Jeong

doi:10.5145/ACM.2019.22.3.71

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Joo, Kwak, Kim, Yoon, and Jeong: Prevalence and Species Spectrum of Pulmonary Nontuberculous Mycobacteria Isolates at a Tertiary Care Center

Original Article

Annals of Clinical Microbiology 2019; 22(3): 71-76.

Published online: 20 September 2019

DOI: https://doi.org/10.5145/ACM.2019.22.3.71

Prevalence and Species Spectrum of Pulmonary Nontuberculous Mycobacteria Isolates at a Tertiary Care Center

Young Sun Joo¹, Na Eun Kwak², Gun Han Kim³, Eun-Jeong Yoon⁴, Seok Hoon Jeong⁴

¹Department of Clnical Pathology, Sangji University College of Science, Wonju, Korea.

²BioPark Diagnostics Inc., Korea.

³Department of Laboratory Medicine, Gangnam Severance Hospital, Korea.

⁴Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.

Correspondence: Eun-Jeong Yoon, Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 211 Eonju-ro, Gangnam-gu, Seoul 06273, Korea. (Tel) 82-2-2019-3783, (Fax) 82-2-2019-3784, ejyoon@yuhs.ac

Received 10 April 2019 Revised 7 May 2019 Accepted 20 May 2019

The Korean Society of Clinical Microbiology

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Pulmonary infection with nontuberculous mycobacteria (NTM) is increasing in South Korea. Since treatment strategy differs by NTM species, accurate identification is necessary. In this study, using Mycobacterium pulmonary isolates recently recovered from a general hospital in Seoul, the prevalence of NTM isolates was investigated.

Methods

A total of 483 Mycobacterium pulmonary strains isolated between May and November 2018 from an 814-bed general hospital in South Korea were analyzed. Bacterial species were identified based on nucleotide sequences of the 16S–23S rDNA internal transcribed spacer and the rpoB gene.

Results

From a total of 1,209 pulmonary specimens from patients suspected to be infected with mycobacteria, 324 deduplicate strains were isolated, comprising 90 Mycobacterium tuberculosis and 229 NTM strains. Among the NTM isolates, 61.5% (n=144) were Mycobacterium avium complex (MAC), including 92 M. avium and 52 Mycobacterium intracellulare, while 8.1% (n=19) represented Mycobacterium abscessus, including 10 M. abscessus subsp. abscessus and 9 M. abscessus subsp. massiliense. In addition, 12 (5.1%) Mycobacterium lentiflavum, 12 (5.1%) Mycobacterium gordonae, 6 (2.6%) Mycobacterium kansasii, and 5 (2.1%) Mycobacterium fortuitum were identified. In addition, Mycobacterium mucogenicum (n=2), Mycobacterium septicum (n=1), Mycobacterium colombiens (n=1), Mycobacterium asiaticum (n=1), and Mycobacterium celatum (n=1) were identified.

Conclusion

Among the recently recovered Mycobacterium pulmonary strains, more than half were identified as NTM, and MAC was the most prevalent NTM, followed by M. abcessuss.

Keywords: Non-tuberculosis mycobacteria, Pulmonary specimen, Species identification

Figures and Tables

Table 1

Primers used in the study

Table 2

Mycobacterial isolates identified in this study

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