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Kim, Park, Hwang, Lee, Song, Lee, Lee, Kim, Kim, Shin, Hong, Shin, Kim, Ryoo, Lee, Jang, and Shin: Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015
Original Article

Annals of Clinical Microbiology 2019; 22(3): 55-60.

Published online: 20 September 2019

DOI: https://doi.org/10.5145/ACM.2019.22.3.55

Serotyping and Antimicrobial Susceptibility of Salmonella Isolated in Korea in 2015

Si Hyun Kim1, *, Eun Hee Park2, *, In Yeong Hwang2, Hyukmin Lee3, Sae Am Song4, Miae Lee5, Seungok Lee6, Soo Young Kim6, Jin Ju Kim7, Jong Hee Shin8, Seong Geun Hong9, Kyeong Seob Shin10, Sunjoo Kim11, Nam Hee Ryoo12, Woonhyoung Lee13, Sook Jin Jang14, Jeong Hwan Shin4, 15

1Department of Clinical Laboratory Science, Semyung University, Jecheon, Korea.

2Department of Infectious Disease, Busan Institute of Health and Environment, Busan, Korea.

3Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea.

4Department of Laboratory Medicine, Inje University College of Medicine, Busan, Korea.

5Department of Laboratory Medicine, Ewha Womans University College of Medicine, Seoul, Korea.

6Department of Laboratory Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea.

7Department of Laboratory Medicine, Inha University College of Medicine, Incheon, Korea.

8Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.

9Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Korea.

10Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, Korea.

11Department of Laboratory Medicine, Gyeongsang National University College of Medicine, Jinju, Korea.

12Department of Laboratory Medicine, Keimyung University College of Medicine, Daegu, Korea.

13Department of Laboratory Medicine, Kosin University College of Medicine, Busan, Korea.

14Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, Korea.

15Paik Institute for Clinical Research, Inje University, Busan, Korea.

Correspondence: Jeong Hwan Shin, Department of Laboratory Medicine, Busan Paik Hospital, Inje University College of Medicine, 75 Bokjiro, Busanjin-gu, Busan 47392, Korea. (Tel) 82-51-890-6475, (Fax) 82-51-890-8615, jhsmile@inje.ac.kr

Equal contributor:

*These authors contributed equally to this work.

Received 27 January 2019       Revised 18 June 2019       Accepted 7 August 2019

© 2019 The Korean Society of Clinical Microbiology

The Korean Society of Clinical Microbiology

(open-access):

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

Salmonella is an important pathogen that causes gastroenteritis and sepsis in humans. Recently, changes in serotype prevalence and an increase in antimicrobial resistance have been reported. This study investigated the distribution of Salmonella serotypes and determined the antimicrobial susceptibility of various strains.

Methods

We collected 113 Salmonella isolates other than Salmonella serotype Typhi from 18 university hospitals in 2015. The serotypes were identified by Salmonella antisera O and H according to the Kauffman White scheme. Antimicrobial susceptibility tests for 12 antibiotics were performed using the disk diffusion method or E-test.

Results

We identified 22 serotypes. Serotype group B (44.2%) was the most common, followed by groups C (34.5%) and D (21.2%). Salmonella I 4,[5],12:i:- (23.0%), S. Enteritidis (16.8%), and S. Typhimurium (12.4%) were the most common species. Resistance rates for ampicillin, chloramphenicol, ceftriaxone, and trimethoprim/sulfamethoxazole were 46.9%, 18.5%, 8.8%, and 5.3%, respectively. The intermediate resistance rate to ciprofloxacin was 29.2%. Six isolates were extended-spectrum β-lactamase (ESBL) producers, including 5 blaCTX-M-15 and 1 blaCTX-M-55.

Conclusion

There have been changes in the serotype prevalence and antimicrobial resistance of Salmonella in Korea, with a high prevalence of CTX-M 15-positive strains. Continuous monitoring of Salmonella serotypes and antimicrobial resistance is warranted.

Keywords: Antimicrobial resistance, Salmonella, Serotype

Figures and Tables

Table 1

Primers used for PCR amplification and sequencing of ESBL genes

acm-22-55-i001
Table 2

Serogroup and serotype distribution of Salmonella isolates

acm-22-55-i002
Table 3

Antimicrobial resistance rates by Salmonella serotype

acm-22-55-i003

*II 6,7:g,[m],s,t:[z42], Agama, Bardo, Dabou, Hindmarsh, Livingstone, Mbandaka, Saintpaul.

Abbreviations: AMC, amoxicillin-clavulanate; AMP, ampicillin; CC, chloramphenicol; IPM, imipenem; CRO, ceftriaxone; GEN, gentamicin; AMK, amikacin; TET, tetracycline; SXT, trimethoprim-sulfamethoxazole; NA, nalidixic acid; AZI, azithromycin; CIP, ciprofloxacin.

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