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Byul Bo Ra Choi1
, Gyoo Cheon Kim2, Jin Woo Hong3, Sang Rye Park4
, Gyoo Cheon Kim2, Jin Woo Hong3, Sang Rye Park4
Abstract
Objectives
To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361).
Methods
Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy.
Results
The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 µg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected.
Figures and Tables
Fig. 1
Effects of Cimicifuga rhizoma in HaCaT (A) and G361 cells (B). Cell viability was measuerd by WST-1 assay (P<0.05).
a,b,cThe same character indication shows that there is no statistical significance.
Acknowledgements
This work was supported by clinical research grant from Pusan National University Hospital in 2018.
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. NRF-2017R1A2B4006654).
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