Web sites on the internet are excellent resources for the younger generation to gain information related to sexuality education. The potential benefits of the information of sexuality education on web sites are obvious. But the information of sexuality education on web sites could also result in potentially negative effects. Yet the quality of the information of sexuality education on web sites is variable and difficult to assess. There is no rating criteria for quality assessment of the information on web sites. The rating criteria for quality assessment of information of sexuality education were investigated and reviewed. Among the criteria, best 15 items to evaluate the information of sexuality education on web sites were selected and identified in this study. 15 items were categorized to reliability (3 items), content (6 items), goal (2 items), design & technology (4 items). This 15-items questionnaires is considered as commonly implementable criteria for the information of sexuality education on web sites in Korean. 20 web sites related to sexuality education were evaluated and the results were discussed.