Nasal defects are commonly caused by trauma or skin cancer. Given that the nose is located in the center of the face and projects from its surface, even a small deformation can be noticeable. For these reasons, when choosing a method for nasal reconstruction both functional and aesthetic factors should be taken into account, including the size and depth of the defect. A variety of methods may be used for nasal reconstruction, including secondary healing, skin grafts, and skin flaps (e.g., local, regional, distant, and free flaps). Skin grafting, one of the most commonly used methods for nasal reconstruction, is highly recommended for superficial defects that do not involve bones or cartilage. Also, it is simple and better reconstruction procedure according to anatomic site, but it can lead to poor aesthetic results due to mismatch of thickness, texture and scar contraction1. Therefore, the donor site for such procedures should be selected keeping in mind similarities in color and texture with the recipient site. In the case of nasal skin grafts, the postauricle area is commonly used as a donor site to minimize donor site morbidity (e.g., scarring). However, the nose can be divided into a number of aesthetic subunits with different characteristics, which should be considered when selecting a donor site for nasal reconstruction.

The donor site for nasal resurfacing with a skin graft should be selected based on the actual quality of the skin. Biophysical parameters are used to estimate regional variations in facial skin2. However, no study of skin grafting has compared nasal skin with donor skin based on biophysical parameters. Therefore, in this study we determined the optimal donor sites for full-thickness skin grafting according to the parts of the nose on the basis of four biophysical parameters: moistness, amount of skin oil, degree of erythema/pigmentation, and wrinkles.

A prospective study was conducted among 30 patients who treated in a dermatology from March 2014 to June 2014. This study protocol received approval from the Institutional Review Board of the Chungnam National University Hospital, Daejon, South Korea (IRB no. CNUH 2013-12-011). Thirty women over the age of 60 (a high-risk group for skin cancer) were selected for this study. Written informed consent was obtained from all participants. We received the patient's consent form about publishing all photographic materials. The age profile of the subjects was from 60 to 84 years (mean age, 69.6 years). Four nasal sites (nasal root, dorsum, tip, and ala) and three donor skin sites (preauricle region, postauricle region, and forehead) were considered. Transepidermal water loss (TEWL) and skin hydration (capacitance) were measured to track the moistness of the areas. The amount of skin oil in each area was estimated based on the lipid content of the skin surface (sebum output). A narrow-band reflectance spectrophotometer was used to assess the erythema/ melanin value. Wrinkling of the skin was assessed using the skin replica technique. In all cases, the measurements were made by the same investigator in a room with a constant temperature (22℃~24℃) and humidity level (40%~45%).

Biophysical parameters such as moistness (TEWL and capacitance), amount of skin oil (sebum output), erythema/melanin value, and wrinkles (skin replica technique) were tested for each donor site on the face and recipient site on the nose (Table 1) and a p-value was obtained for each of the test results (Table 2).

According to the p-value for each biophysical parameter at the donor sites (forehead, preauricle region, and postauricle region) and recipient sites (nasal root, dorsum, tip, and ala), the highest value from the donor site was selected as the most similar part to each area of the nose. The nasal root was correlated with the forehead in terms of TEWL (p=0.18) and sebum output (p=0.46) and with the postauricle region in terms of the capacitance (p=0.93) and skin replica measurements (p=0.70). The nasal dorsum was correlated with the preauricle region in terms of TEWL (p=0.73), erythema/melanin value (p=0.12), and skin replica measurements (p=0.16). The nasal tip was correlated with the preauricle region in terms of TEWL (p=0.82), sebum output (p=0.07), erythema/melanin value (p=0.70), and skin replica measurements (p=0.12). The ala was correlated with the forehead in terms of TEWL (p=0.63) and skin replica measurements (p=0.28) (Table 2).

The highest value for each biophysical parameter at the donor site was taken to indicate similarity with the recipient site. The forehead and postauricle region were very similar to the nasal root, while the preauricle region was very similar to the nasal dorsum and tip. The alar region was most similar to the forehead.

The nose may be divided into five subunits (dorsum, root, lateral wall, tip, and ala) based on the anatomic outline of the nose, and into three zones based on the thickness of the skin. Zone 1 is soft and thin (the nasal dorsum and lateral wall have nearly no sebaceous glands). Zone 2 is stiff and thick (the nasal tip, supratip area, and ala have numerous sebaceous glands). Like Zone 1, Zone 3 is soft and thin. The alar margin, soft triangle, and columella, which have nearly no sebaceous glands, do not move easily because they are fixed to deeper structures, such as cartilage. In selecting the donor site for a full-thickness skin graft, the preauricular area, which has a thin wall and few sebaceous glands, is suitable for Zones 1 and 3. Given the texture of the skin and the distribution of sebaceous glands, the forehead is a suitable donor site for Zone 211. These guidelines are based on anatomical and histological features, but do not reflect the actual state of the skin, such as moistness, amount of skin oil, wrinkles, and erythema. We selected a possible full-thickness skin graft donor site for each part of the nose from the most similar facial area by applying biophysical parameters that reflect the actual state of the skin. These parameters included moistness, indicating the moisture content of the skin surface, amount of skin oil, indicating the functional state of the sebaceous glands, the degree of erythema/pigmentation, reflecting the color of the skin, and wrinkles, indicating the outline of the skin surface.

The stratum corneum maintains skin homeostasis by regulating body fluids and the evaporation of water. The water content of the stratum corneum in a skin graft determines the flexibility of the graft; thus, matching the texture of the skin graft with the microstructure of the skin surface is one of the most important factors in performing a skin graft. TEWL is an indicator of the function of the stratum corneum and reflects the amount of the moisture lost to the outside from the skin, but it does not directly represent the moisture of the stratum corneum and is closely related to the barrier function and moisture preservation function of skin. By determining the rate of diffusion of moisture from the skin surface into the air using Fick's law, the amount of moisture evaporated from the epidermis can be estimated312. Capacitance can be used as another indicator of moistness. Using the fact that the resistance from the skin surface is inversely related to moisture, one can determine the relative size of the water content by measuring the capacitance413.

The skin's lipid layer, which is derived from the sebaceous glands or stratum corneum, is an essential feature of skin that affects its barrier function and the evaporation of water and which can be used as a marker of sebum secretion based on the amount of skin oil. The secretion of sebum can be measured based on the fact that if a film gets stained with lipid, the transparency is higher. By measuring the brightness of the film after 30 s of contact at a constant pressure, the amount of lipid present may be assessed5. Vascularity (erythema)/pigmentation can be measured using a spectrophotometer, which takes advantage of the fact that hemoglobin/melanin, which determine skin color, have unique optical absorption spectra. A narrow-band reflectance spectrophotometer is a device for measuring light reflected from the skin surface at wavelengths of 567 nm (green), 660 nm (red), and 880 nm (infrared) were used in this study. By calculating the light intensity absorbed into the skin and reflected from the skin, the erythema and melanin value were calculated. Erythema and melanin value expressed vascularity and pigmentation respectively. And the Mexameter® is an objective skin color-measuring device. It has been reported that this instrument is highly sensitive enough to quantify small differences in skin color678.

After manufacturing replicas of the skin, quantitative measurements of wrinkles in the skin were made using a dual-wavelength phase meter (an interferometric measuring device for dual-wavelength phase differences). This method of evaluating skin wrinkles has been approved by the Korean Food and Drug Administration910.

In conclusion, the biophysical parameters at the nasal root, forehead, and postauricle region were very similar. The preauricle region was most similar to the nasal dorsum and nasal tip, while the alar region was most similar to the forehead. These results will be useful in donor site selection for skin grafts in patients with nasal defects.

In this study, biophysical measurements were collected to judge the similarities between full-thickness skin graft recipient sites and donor sites. The results may be used as a meaningful guide for donor site selection. However, the importance of each indicator was not addressed. Also, the evaluations in this study were performed preoperatively using only skin parameters; no evaluations were conducted after placement of the skin graft, postoperatively. Further, this study did not address the effect of sex. Especially, the condition of the skin can be affected by various conditions before surgery and during the healing conditions such as inflammation, ultraviolet (UV) exposure, etc. In that respect, donor and recipient skin cannot be perfect matched by comparative measurement. Additional studies are needed to overcome these limitations.