Journal List > Korean J Occup Environ Med > v.11(4) > 1126127

Wang, Cha, Roh, Shin, Kim, and Jeon: The variation of thiodiglycolic acid concentration in urine with vinyl chloride exposure levels in rats

Abstract

OBJECTIVES

It is the objective of this research to identify the variation of thiodiglycolic acid (TdGA) in urine with vinyl chloride monomer (VCM) exposure levels through methylation.

METHODS

After rats were exposed to vinyl chloride monomer of 4 levels, 0 mg/m3, 50 mg /m3, 150 mg/m3, 500 mg/m3, respectively, of which urine was sampled in each sampling time of 0 hour, 2 hours, 4 hours, 8 hours, 16 hours, 24 hours, 36 hours, 48 hours. After urine had been methylated with diazomethane in the preliminary experiment and the peak of 146 m/z had been verified, the main experiment was done.

RESULTS

In the variation of TdGA with sampling times, concentration of TdGA increased rapidly in 4 hours and then decreased after 8 hours. When the variation of urinary .TdGA concentration in urine according to exposure level of VCM was verified through Kruskal-Wallis statistical method at each sampling time, the significant increment with the exposure levels at 2 hours, 4 hours, 8 hours after exposure was clarified.

CONCLUSION

TdGA concentration in urine with increment of VCM exposure level increased, especially significantly at 2 hours, 4 hours, 8 hours of sampling time.

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