Journal List > Korean J Occup Environ Med > v.11(4) > 1126127

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Wang, Cha, Roh, Shin, Kim, and Jeon: The variation of thiodiglycolic acid concentration in urine with vinyl chloride exposure levels in rats
Original Article

Korean Journal of Occupational and Environmental Medicine 1999; 11(4): 431-438.

Published online: 31 January 2019

DOI: https://doi.org/10.35371/kjoem.1999.11.4.431

The variation of thiodiglycolic acid concentration in urine with vinyl chloride exposure levels in rats

Seung Jun Wang, Bong Suk Cha, Jae Hoon Roh, Dong Chun Shin, Myung Su Kim, Keun Jae Jeon

Department of Preventive Medicine and Institute of Occupational Medicine, Wonju College of Medicine Yonsei University, Korea.

Department of Preventive Medicine and Public Health, Institute of Occupational Health, College of Medicine Yonsei University, Korea.

Doping Control Center, Korean Institute of Science and Technology, Korea.

Copyright © 1999 The Korean Society of Occupational and Environmental Medicine

Abstract

OBJECTIVES

It is the objective of this research to identify the variation of thiodiglycolic acid (TdGA) in urine with vinyl chloride monomer (VCM) exposure levels through methylation.

METHODS

After rats were exposed to vinyl chloride monomer of 4 levels, 0 mg/m3, 50 mg /m3, 150 mg/m3, 500 mg/m3, respectively, of which urine was sampled in each sampling time of 0 hour, 2 hours, 4 hours, 8 hours, 16 hours, 24 hours, 36 hours, 48 hours. After urine had been methylated with diazomethane in the preliminary experiment and the peak of 146 m/z had been verified, the main experiment was done.

RESULTS

In the variation of TdGA with sampling times, concentration of TdGA increased rapidly in 4 hours and then decreased after 8 hours. When the variation of urinary .TdGA concentration in urine according to exposure level of VCM was verified through Kruskal-Wallis statistical method at each sampling time, the significant increment with the exposure levels at 2 hours, 4 hours, 8 hours after exposure was clarified.

CONCLUSION

TdGA concentration in urine with increment of VCM exposure level increased, especially significantly at 2 hours, 4 hours, 8 hours of sampling time.

Keywords: Vinyl chloride monomer, Thiodiglycolic acid

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