Journal List > Korean J Gynecol Oncol Colposc > v.15(3) > 1123567

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Kwon, Kim, Kim, Na, Kim, Lee, Ko, and Lee: Establishment of General Primers and Oligo-DNA Chip for the Detection and Typing of Human Papillomavirus
Original Article

Korean Journal of Gynecologic Oncology and Colposcopy 2004; 15(3): 231-239.

Published online: 12 March 2019

DOI: https://doi.org/10.3802/kjgoc.2004.15.3.231

Establishment of General Primers and Oligo-DNA Chip for the Detection and Typing of Human Papillomavirus

Ji Eun Kwon, Yeon Soo Kim, Seung Jo Kim, Young Jeong Na, In Ho Kim, Sun Young Lee, Jung Jae Ko, Sang Hwa Lee

Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University, Sungnam, Gyeonggi-do, Korea.

Comprehensive Gynecologic Cancer Center, Bundang Cha Hospital, Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University, Sungnam, Gyeonggi-do, Korea.

Copyright © Korean Society of Gynecologic Oncology and Colposcopy

Abstract

OBJECTIVE

To establish the use of a HPV oligo-DNA chip for early diagnosis and prognosis assessment in cervical cancer.

METHODS

HPV PCR general primers and a oligo-DNA chip have been constructed by using HPV genomic sequences on the webs and computer programs. With a total of 344 random cervical scrapes, their efficacies were explored based on pathology.

RESULTS

All subjects (n=48) with diagnosis of low squamous intraepithelial lesion (LSIL) were positives for both HPV PCRs and oligo-DNA chip tests, whereas 44 out of 286 subjects (264 cases of NIL and 22 cases of ASCUS) were detected HPV positives. The proportion of each genotypes in HPV positive subjects were 38.0% (HPV-16), 16.3% (HPV-58), 9.8% (HPV-52) and 35.9% (others).

CONCLUSION

This study gives favorable result to establish a novel method using HPV oligo-DNA chip with PCR general primers, based on the clinical trials in random subjects although the scale is small in size. In the future, a diverse of comparative and systematic clinical studies should be followed.

Keywords: Human papillomavirus, General primer, HPV oligo-DNA chip, Cervical cancer

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