Journal List > Korean J Gynecol Oncol Colposc > v.14(4) > 1123532

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Kim, Bae, Seo, Kim, Lee, Kim, Lee, Namkoong, Kim, and Ahn: Changes of Gene Expression between HaCat and SiHa cells using GeneFishingTM PCR
Original Article

Korean Journal of Gynecologic Oncology and Colposcopy 2003; 14(4): 300-305.

Published online: 12 March 2019

DOI: https://doi.org/10.3802/kjgoc.2003.14.4.300

Changes of Gene Expression between HaCat and SiHa cells using GeneFishingTM PCR

Byung Hoon Kim, Su Min Bae, Min Jae Seo, Young Wan Kim, Jung Woong Lee, Young Wook Kim, Joon Mo Lee, Sung Eun Namkoong, Chong Kook Kim, Woong Shick Ahn

Catholic Research Institutes of Medical Science, College of Medicine, The Catholic University of Korea, Korea.

Department of Obstetrics and Gynecology, College of Medicine, The Catholic University of Korea, Korea.

College of Pharmacy, Seoul National University, Korea.

Copyright © Korean Society of Gynecologic Oncology and Colposcopy

Abstract

OBJECTIVE

The purpose of this study is investigated the differentially expressed genes between normal and cervical cancer cell line.

METHODS

We used normal human keratinocyte (HaCaT) as a control and HPV-16 positive cervical cancer (SiHa) cell line. Two cell lines were studied differential expressed genes by using GeneFishingTM PCR and analyzed with BLAST search.

RESULTS

As compared with normal, cervical cancer cell line was showed 25 up-regulated genes including the S1-2-2, S5-1 and 24 down-regulated genes.

CONCLUSION

GeneFishTM PCR test is very sensitive and effective method for detection of changed gene expression. We could search differentially expressed genes between normal and cervical cancer cell line. In the future, we need to research various genes function to participate in the process of tumor development and progression.

Keywords: HaCaT, SiHa, Cervical cancer, Differential expressed genes (DEGs), GeneFishingTM PCR

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