Analysis of expression of survivin, caspase 3, and p53 protein in cervical neoplasia comparing with Ki-67 index

Ji-Young Cha; Tai-Il Cho; Tae-Yang Park; Jae-Sung So; Se-Kyoung Choi; Eon-Sub Park

doi:10.3802/kjgo.2008.19.1.17

Journal List > Korean J Gynecol Oncol > v.19(1) > 1123454

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Cha, Cho, Park, So, Choi, and Park: Analysis of expression of survivin, caspase 3, and p53 protein in cervical neoplasia comparing with Ki-67 index

Original Article

Korean Journal of Gynecologic Oncology

Korean Journal of Gynecologic Oncology 2008; 19(1): 17-25.

Published online: 20 March 2008

DOI: https://doi.org/10.3802/kjgo.2008.19.1.17

Analysis of expression of survivin, caspase 3, and p53 protein in cervical neoplasia comparing with Ki-67 index

Ji-Young Cha¹, Tai-Il Cho¹, Tae-Yang Park¹, Jae-Sung So¹, Se-Kyoung Choi¹, Eon-Sub Park²

¹Department of Obstetrics and Gynecology, Sungae Hospital, Seoul, Korea.

²Department of Pathology, College of Medicine,Chung-Ang University, Seoul, Korea.

Address reprint requests to Eon-Sub Park. Department of Pathology, College of Medicine, Chung-Ang University 221, Heukseok-dong, Dongjak-gu, Seoul 516-756, Korea. Tel: 82-2-820-5679, Fax: 82-2-813-5387, esp@cau.ac.kr

Received 29 October 2007 Accepted 11 February 2008

Abstract

Objective

The aim of this study was to determine the role of survivin, caspase 3, p53 and Ki-67 expression in the carcinogenesis of cervical carcinoma and aggressiveness of cervical intraepithelial neoplasia (CIN).

Methods

The pathology specimens of 94 patients with a diagnosis of Low grade CIN (31 cases), High grade CINL (32 cases) and squamous cell carcinoma (31 cases) were evaluated immunohistochemically for the expression of survivin, caspase 3, p53 and Ki-67 in paraffin sections.

Results

Survivin, p53 and Ki-67 expressions were progressively increased in accordance with the increasing degree of malignancy, but caspase 3 immunoreactivity was higher in high grade CIN than in low grade CIN and invasive cervical cancers. There was no significant difference between Ki-67 index and survivin, caspase 3 and p53 expression with the increasing degree of malignancy. The Ki-67 index was closely related to p53 overexpression in invasive cervical carcinoma group.

Conclusion

A sequential increase of survivin, p53, and Ki-67 was observed in paralleling the progression of grade of CIN and cervical cancer. In addition, caspase 3 expression increased proportionally to the low-grade CIN to high grade CIN.

Keywords: Survivin, Caspase 3, p53, Ki-67, Cervical neoplasia

Figures and Tables

Fig. 1

A low-grade cervical intraepithelial neoplasia showing survivin-positive cells scattered in the lower third of cervix (×200).

Fig. 2

A high-grade cervical intraepithelial neoplasia showing nuclear and cytoplasmic staining of caspase 3 (×200).

Fig. 3

Nuclear immunreactivity of p53 in a poorly differentiated invasive squamous cell carcinoma of cervix (×200).

Fig. 4

Immunostaining of Ki-67 showing nests of squamous cell carcinoma (×200).

Table 1

Expression of survivin, caspase 3 and p53 ptrotein in the squamous neoplasia of uterine cervix

CIN; cervical intraepithelial neoplasia, SCC; squamous cell carcinoma

Table 2

Ki-67 index in the squamous neoplasia of uterine cervix

CIN; cervical intraepithelial ceoplasia, SCC; squamous cell carcinoma

Table 3

Relationship between Ki-67 labelling index and survivin, caspase 3, and p53 in low grade cervical intraepithelial neoplasia

Table 4

Relationship between Ki-67 labelling index and survivin, caspase 3, and p53 in high grade cervical intraepithelial neoplasia

Table 5

Relationship between Ki-67 labelling index and survivin, caspase 3, and p53 in squamous cell carcinoma of uterine cervix

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