Abstract
Purpose
Materials and Methods
Results
ACKNOWLEDGEMENTS
Notes
Author Contributions:
Conceptualization: Yun-Gyoung Hur, Sang-Nae Cho.
Data curation: Yun-Gyoung Hur, Ji Young Hong, Dong Hwan Choi.
Formal analysis: Yun-Gyoung Hur.
Funding acquisition: Sang-Nae Cho.
Investigation: Yun-Gyoung Hur, Ji Young Hong, Dong Hwan Choi, Ahreum Kim, Soyeong Park, Minsuk Kwon, Kyungjoon Kang, Jeong-Min Lee, Yoonsuk Lee.
Methodology: Yun-Gyoung Hur, Ji Young Hong, Dong Hwan Choi, Hoodon Joo, Sang-Nae Cho.
Project administration: Yun-Gyoung Hur.
Resources: Yun-Gyoung Hur, Ji Young Hong, Sang-Nae Cho.
Software: Jeong-Min Lee, Hoodon Joo.
Supervision: Yun-Gyoung Hur, Sang-Nae Cho.
Validation: Yun-Gyoung Hur, Ji Young Hong, Dong Hwan Choi, Ahreum Kim.
Visualization: Yun-Gyoung Hur.
Writing—original draft: Yun-Gyoung Hur.
Writing—review & editing: Yun-Gyoung Hur, Hazel M. Dockrell.
References
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Fig. 1
Diagnostic value of the ichroma™ IGRA-TB test in identifying M. tb infection. The IFN-γ responses measured by ichroma™ IGRA-TB cartridges distinguished infected individuals with an AUC of 0.9706 (95% CI, 0.92–1.00, p<0.001) in ROC curve analysis (p<0.001). IGRA, IFN-γ release assay; TB, tuberculosis; CI, confidence interval; AUC, area under the curve; ROC, receiver operating characteristic.
![ymj-60-375-g001](/upload/SynapseXML/0069ymj/thumb/ymj-60-375-g001.jpg)
Fig. 2
Association of IFN-γ release between two different assays. The association of the IFN-γ responses between the QFT-GIT and ichroma™ IGRA-TB tests was analyzed by Spearman's correlation coefficient. The graph shows a very strong positive correlation of IFN-γ values between the two tests (r=0.91, p<0.001). QFT-GIT, QuantiFERON-TB Gold In-Tube; IGRA, IFN-γ release assay; TB, tuberculosis.
![ymj-60-375-g002](/upload/SynapseXML/0069ymj/thumb/ymj-60-375-g002.jpg)
Table 1
Characteristics of the Study Participants
![ymj-60-375-i001](/upload/SynapseXML/0069ymj/thumb/ymj-60-375-i001.jpg)
Table 2
Percent Positivity and Percent Negativity by QFT-GIT and ichroma™ IGRA-TB Tests in Healthy Subjects
![ymj-60-375-i002](/upload/SynapseXML/0069ymj/thumb/ymj-60-375-i002.jpg)
Assay type | % positivity (n) | % negativity (n) |
---|---|---|
QFT-GIT IFN-γ ELISA | 53.3 (32) | 46.7 (28) |
ichroma™ IGRA-TB | 48.3 (29) | 51.7 (31) |
Table 3
Subjects Who Showed Differences in IFN-γ Positivity between the QFT-GIT and ichroma™ IGRA-TB Tests
![ymj-60-375-i003](/upload/SynapseXML/0069ymj/thumb/ymj-60-375-i003.jpg)
Sample | QFT-GIT ELISA | ichroma™ IGRA-TB cartridge | ||
---|---|---|---|---|
Result | IFN-γ (IU/mL) | Result | IFN-γ (IU/mL) | |
1 | Positive | 0.42 | Negative | 0.16 |
2 | Positive | 0.57 | Negative | -0.22 |
3 | Positive | 0.95 | Negative | 0.12 |
QFT-GIT, QuantiFERON-TB Gold In-Tube; ELISA, enzyme-linked immunosorbent assay; IGRA, IFN-γ release assay; TB, tuberculosis.
The three positive IFN-γ responders on the QFT-GIT test were weakly positive (≥0.35 but <1.0 IU/mL), and those responders did not show positive responses on the test with the ichroma™ IGRA-TB cartridge (≤0.21 IU/mL).