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Kim, Kim, Oh, Kwak, Kim, Kang, Cho, Shim, and Yoon: Cytotoxic Constituents from the Roots of Asarum sieboldii in Human Breast Cancer Cells
Original Article

Natural Product Sciences 2019;25(1):72-75.

Published online: 17 December 2019

DOI: https://doi.org/10.20307/nps.2019.25.1.72

Cytotoxic Constituents from the Roots of Asarum sieboldii in Human Breast Cancer Cells

Eunae Kim1, Hyun Jung Kim2, Ha-Na Oh2, Ah-Won Kwak2, Su-Nam Kim3, Bok Yun Kang4, Seung-Sik Cho2, Jung-Hyun Shim2, ∗, Goo Yoon2, ∗

1College of Pharmacy, Chosun University, Gwangju 61452, Republic of Korea

2College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, South Korea

3KIST Gangneung Institute, Gangneung 25451, Republic of Korea

4College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea

∗Author for correspondence Jung-Hyun Shim, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, South Korea Tel: +82-61-450-2684; E-mail: s1004jh@gmail.com Goo Yoon, College of Pharmacy, Mokpo National University, Muan, Jeonnam 58554, South Korea Tel: +82-61-450-2682; E-mail: gyoon@mokpo.ac.kr

Received 26 July 2018       Revised 29 November 2018       Accepted 1 December 2018

Copyright © 2019 The Korean Society of Pharmacognosy

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Bioassay-guided fractionation of the roots of Asarum sieboldii led to the isolation of the six compounds methylkakuol (1), sesamin (2), asarinin (3), xanthoxylol (4), and (2E,4E,8Z,10E/Z)-N-(2-methylpropyl) dodeca-2,4,8,10-tetraenamide (5/6). Among the isolates, xanthoxylol (4) exhibited significant cytotoxicity against human breast cancer cells MCF-7 and MDA-MB-231 in vitro with IC50 values of 9.15 and 13.95 µM, respectively.

Keywords: Asarum sieboldii, Cytotoxicity, Breast cancer, Xanthoxylol

REFERENCES

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Fig. 1.
Structures of isolated compounds (1–6) from A. sieboldii.
nps-25-72f1.tif
Table 1.
The anti-proliferative effect of compounds (1–6) on human breast cancer cells (MCF-7 and MDA-MB-231).
Compounds MCF-7 (IC50, µ M) MDA-MB-231 (IC50, µ M)
Methylkakoul (1) > 100 > 100
Sesamin (2) 98.57 > 100
Asarinin (3) 67.25 > 100
Xanthoxylol (4) 9.15 13.95
(2 E,4 E,8 Z,10 E)-N-(2-methylpropyl)dodeca-2,4,8,10-tetraenamide (5) > 100 > 100
(2 E,4 E,8 Z,10 Z)-N-(2-methylpropyl)dodeca-2,4,8,10-tetraenamide (6) > 100 > 100
5-Fluorouracil 42.09 27.19
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