The study was carried out to investigate the prevalence of Brucella antibodies in sera of 120 cows in Bangladesh Agricultural University Dairy Farm and adjacent villages, Bangladesh. The epidemiological history and blood was collected from the cows. The serum samples were subjected to Rose Bengal Test (RBT)and plate agglutination test (PAT) for initial screening of Brucella antibodies and the positive sera samples were then subjected to tube agglutination test (TAT)for further confirmation. The higher rate of Brucella antibody was recorded in rural farm (5.0%)than organized farm (2.5%)and in pregnant cows (5.9%)than non-pregnant cows (4.7%). A total of 3 (4%)Brucella positive antibody cases were recorded in cows of above four years of age whereas, 1 (2.3%)positive case was found in cows of less than 4 years of age. The study revealed that number of Red Shindi was the highest and the prevalence of brucellosis in Bangladesh cow population is not negligible and it is worthwhile to consider adoption of preventive measures.