We conducted an experiment to investigate the histologic chronologic changes of tendon to bone fixation using the Coles method, the role of periosteum to the tenodesis and also to find a clinical relevance of this technique for tumor salvage operation. The periosteum around the tenodesis was excised in group I, but it was incised longitudinally and sutured to tendon in group II. As a result, the tendons in the marrow cavity of tibia were firmly fixed by fibrosseous metaplasia in group I at 8 weeks after experiment, but not formed the fibrocartilagenous layer in both group as a transitional zone of internal stress transmission from tendon to bone in normal tendon to insertion site, and the group II show the firm connection between sutured periosteum and transferred tendon by external callus after 3 weeks. These mean the periosteum should be sutured over the inserted tendon to get .the early rigid fixation by the induction of external callus around the edges of the tendon to bone insertion. As a clinical trial, the tendon to implant fixation by screws as a common conventional method will fail to get the active joint motion after limb salvage operation for malignant bone and joint tumor, and so tendon-bone-porous coated implant fixation should be considered.