Abstract
The three flavone glycosides, 4′-O-methylisoscutellarein 7-O-(6″′-O-acetyl)-β-D-allopyranosyl(1 → 2)-β-D-glucopyranoside (1), isoscutellarein 7-O-(6″′-O-acetyl)-β-D-allopyranosyl(1 → 2)-β-D-glucopyranoside (3), and isoscutellarein 7-O-β-D-allopyranosyl(1 → 2)-β-D-glucopyranoside (4) in addition to a flavonol glycoside, kaempferol 3-O-β-D-glucopyranoside (astragalin, 2), were isolated from Stachys japonica (Lamiaceae). In cholinesterase inhibition assay, compound 1 significantly inhibited aceylcholinesterase (AChE) and butyrylcholinesterase (BChE) activities (IC50 s, 39.94 μ g/ml for AChE and 86.98 μ g/ml for BChE). The content of isolated compounds were evaluated in this plant extract by HPLC analysis. Our experimental results suggest that the flavonoid glycosides of S. japonica could prevent the memory impairment of Alzheimer's disease.
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Table 1.
Table 2.
Table 3.
Samples | AChEa | BChE |
---|---|---|
Mean ± SEM | Mean ± SEM | |
Aq. MeOH extract | 86.09 ± 1.27 | 172.88 ± 0.71 |
CHCl3 fraction | 68.66 ± 1.32 | 133.96 ± 2.43 |
BuOH fraction | 74.20 ± 2.20 | 117.94 ± 7.61 |
1 | 39.94 ± 0.76 | 86.98 ± 1.72 |
2 | 66.76 ± 2.82 | > 200 |
3 | 65.40 ± 0.45 | 109.76 ± 2.79 |
4 | 59.55 ± 2.92 | 160.84 ± 3.26 |
2a | 32.19 ± 0.82 | 52.34 ± 1.51 |
3a | 58.19 ± 1.11 | 79.60 ± 0.28 |
Apigeninb | 12.80 ± 0.37 | 9.18 ± 0.67 |
Table 4.
Compound | t R (min) | Calibration equation (linear model)a | Linear range (µg/ml) | R2b | LODc (µg/ml) | LOQd (µg/ml) |
---|---|---|---|---|---|---|
1 | 20.11 | y = 157.68 x + 113.14 | 7.81–250.0 | 0.999 | 1.18 | 3.95 |
2 | 16.92 | y = 378.45 x + 106.78 | 7.81–250.0 | 0.999 | 0.51 | 1.70 |
2a | 22.10 | y = 957.20 x + 158.03 | 7.81–250.0 | 0.999 | 0.15 | 0.49 |
3 | 17.22 | y = 278.85 x + 122.48 | 7.81–250.0 | 0.999 | 0.63 | 2.12 |
3a | 17.98 | y = 313.95 x + 86.27 | 7.81–250.0 | 0.999 | 0.68 | 2.27 |
4 | 14.83 | y = 95.75 x + 55.73 | 7.81–250.0 | 0.999 | 2.03 | 6.76 |