Inactivation Efficacy of a Non-thermal Atmospheric Pressure Plasma Generator against Mycobacterium tuberculosis

Noori Lee; Sanghee Park; Jiyeon Kim; Keyyoung Kim; Daeyeon Kim

doi:10.14192/kjhaicp.2018.23.2.80

Journal List > Korean J Healthc Assoc Infect Control Prev > v.23(2) > 1110778

Go to TopGo to Top Go to BottomGo to Bottom

TOOLS

Lee, Park, Kim, Kim, and Kim: Inactivation Efficacy of a Non-thermal Atmospheric Pressure Plasma Generator against Mycobacterium tuberculosis

Original Article

Korean Journal of Healthcare-Associated Infection Control and Prevention 2018; 23(2): 80-85.

Published online: 27 December 2018

DOI: https://doi.org/10.14192/kjhaicp.2018.23.2.80

Inactivation Efficacy of a Non-thermal Atmospheric Pressure Plasma Generator against Mycobacterium tuberculosis

Noori Lee¹, Sanghee Park¹, Jiyeon Kim¹, Keyyoung Kim², Daeyeon Kim³

¹Clinical Research Center, Masan National Tuberculosis Hospital, Changwon, Korea.

²Institute of Environmental and Industrial Medicine, Hanyang University, Seoul, Korea.

³Masan National Tuberculosis Hospital, Changwon, Korea.

Correspondence to: Daeyeon Kim, Masan National Tuberculosis Hospital, 215 Gapo-ro, Masanhappo-gu, Changwon 51755, Korea. Tel: 055-249-5001, Fax: 055-249-5004, stoptb@korea.kr

Received 3 June 2018 Revised 2 July 2018 Accepted 4 July 2018

Korean Society for Healthcare-Associated Infection Control and Prevention

Abstract

Background

Tuberculosis is an infectious disease, -spreading from person to person through the air. When a person with infectious tuberculosis coughs or sneezes, tiny particles containing Mycobacterium tuberculosis are expelled into the air and remain suspended for several hours. Therefore, it is important to control the transmission of M. tuberculosis through air. This study was conducted to determine the inactivation efficacy of the plasma generator against the M. tuberculosis.

Methods

The attenuated M. tuberculosis H37Ra inoculated on the solid medium were placed in hospital wards and BL3 laboratory, with a plasma generator (Model TB-500, Shinyoung Airtech, Seongnam-si, Korea) being operated at specific time intervals. The growth of M. tuberculosis was determined by plasma exposure time.

Results

M. tuberculosis samples exposed to non-thermal plasma for 3, 6, 9, and 24 h showed inhibition effects of 45–75%, 69–86%, 93–100%, and 100%, respectively. Thus, it was found that the inhibition effect of plasma generators against M. tuberculosis were proportional to the plasma exposure time.

Conclusion

The non-thermal atmospheric pressure plasma generator technique may be applied as a promising technique for the prevention of M. tuberculosis infection in healthcare and other public facilities, when operated for more than 9 h.

Keywords: Inactivation, Mycobacterium tuberculosis, Plasma, Tuberculosis

Figures and Tables

Fig. 1

Inhibition of Mycobacterium tuberculosis growth by time of exposure to plasma.

Fig. 2

Inhibition of Mycobacterium tuberculosis growth by location.

Fig. 3

Inhibition of Mycobacterium tuberculosis growth by innoculated bacterial concentration.

Table 1

Inhibition of Mycobacterium tuberculosis growth by location and time exposed to plasma

^*t-test.

Table 2

Inhibition of Mycobacterium tuberculosis growth by concentration of initial innoculation

^*t-test. ^†Not Applicable.

References

1. Health Quality Ontario. Air cleaning technologies: an evidence-based analysis. Ont Health Technol Assess Ser. 2005; 5:1–52.

2. Bär W, Márquez de Bär G, Naumann A, Rüsch-Gerdes S. Contamination of bronchoscopes with Mycobacterium tuberculosis and successful sterilization by low-temperature hydrogen peroxide plasma sterilization. Am J Infect Control. 2001; 29:306–311.

3. Kyi MS, Holton J, Ridgway GL. Assessment of the efficacy of a low temperature hydrogen peroxide gas plasma sterilization system. J Hosp Infect. 1995; 31:275–284.

4. Venezia RA, Orrico M, Houston E, Yin SM, Naumova YY. Lethal activity of nonthermal plasma sterilization against microorganisms. Infect Control Hosp Epidemiol. 2008; 29:430–436.

5. Lloyd G, Friedman G, Jafri S, Schultz G, Fridman A, Harding K. Gas plasma: medical uses and developments in wound care. Plasma Process Polym. 2010; 7:194–211.

6. Steenken W, Oatway WH, Petroff SA. Biological studies of the tubercle bacillus : III. Dissociation and pathogenicity of the R and S variants of the human tubercle bacillus (H(37)). J Exp Med. 1934; 60:515–540.

7. Fontes B, Cattani Heimbecker AM, de Souza Brito G, Costa SF, van der Heijden IM, Levin AS, et al. Effect of low-dose gaseous ozone on pathogenic bacteria. BMC Infect Dis. 2012; 12:358.

8. Zimmermann JL, Shimizu T, Schmidt HU, Li YF, Morfill GE, Isbary G. Test for bacterial resistance build-up against plasma treatment. New J Phys. 2012; 14:073037.

9. Vladimirsky MA, Shipina LK, Makeeva ES, Alyapkina YS, Mikheev AY, Morozov VN. Application of water-soluble nanofilters for collection of airborne Mycobacterium tuberculosis DNA in hospital wards. J Hosp Infect. 2016; 93:100–104.

10. Lee ES, Yoon TH, Lee MY, Han SH, Ka JO. Inactivation of environmental mycobacteria by free chlorine and UV. Water Res. 2010; 44:1329–1334.

11. Riley RL, Knight M, Middlebrook G. Ultraviolet susceptibility of BCG and virulent tubercle bacilli. Am Rev Respir Dis. 1976; 113:413–418.

12. Kahnert A, Seiler P, Stein M, Aze B, McDonnell G, Kaufmann SH. Decontamination with vaporized hydrogen peroxide is effective against Mycobacterium tuberculosis. Lett Appl Microbiol. 2005; 40:448–452.

13. Krueger AP, Reed EJ. Biological impact of small air ions. Science. 1976; 193:1209–1213.

14. Shimizu T, Zimmermann JL, Morfill GE. The bactericidal effect of surface micro-discharge plasma under different ambient conditions. New J Phys. 2011; 13:023026.

15. Daeschlein G, Napp M, Majumdar A, Richter E, Rüsch-Gerdes S, Aly F, et al. In vitro killing of mycobacteria by low temperature atmospheric pressure plasma and dielectric barrier discharge plasma for treatment of tuberculosis. Clin Plasma Med. 2017; 5-6:1–7.

TOOLS

Similar articles