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Lee, Ko, Kim, Lee, Baek, and Kim: Comparison among Diagnostic Methods of Rotaviral Gastroenteritis in Children
Original Article

Korean Journal of Pediatric Gastroenterology and Nutrition 2001; 4(1): 34-40.

Published online: 30 April 2001

DOI: https://doi.org/10.5223/kjpgn.2001.4.1.34

Comparison among Diagnostic Methods of Rotaviral Gastroenteritis in Children

Jang Hun Lee, Eun Young Ko1, Jae Oong Kim2, Jung Hwa Lee, Lack Ju Baek1, Soon Kyum Kim

Department of Pediatrics, College of Medicine, Korea University, Seoul, Korea.

1Department of Microbiology, College of Medicine, Korea University, Seoul, Korea.

2Department of Pediatrics, Seoul Adventist Hospital, Seoul, Korea.

leejmd@chollian.net

Copyright © 2001 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition

Abstract

PURPOSE

The purpose of this study was to evaluate the clinical efficacy of reverse transcription- polymerase chain reaction (RT-PCR) in detecting rotaviral gastroenteritis in children comparing with that of commercial immunoassays.

METHODS

Stools from 79 children admitted Korea University Hospital due to diarrhea were collected from December 1999 to February 2000. Immunoassays were done using commercial rotavirus Latex kit and Rotatec (ELISA) kit. RT-PCR was performed to amplify group A rotavirus, most commonly pathogenic to human, using VP4- and VP7-specific primers. The detection rates of immunoassays and RT-PCR were compared.

RESULTS

ELISA assay was superior to LA assay and moderately concordant with RT-PCR in detecting rotaviral gastroenteritis.

CONCLUSION

Although RT-PCR is known very sensitive, it does not have significant advantage over immunoassay in detecting rotaviral gastroenteritis.

Keywords: Rotavirus, Gastroenteritis, Diagnosis, RT-PCR, ELISA, Latex agglutination, Child

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