Journal List > Korean J Pediatr Gastroenterol Nutr > v.4(2) > 1110477

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Ahn, Kim, Lee, Hwang, and Choe: Helicobacter pylori vacA Mosaicism and New Primers for vacA Signal Sequence Indigenous to Korea
Original Article

Korean Journal of Pediatric Gastroenterology and Nutrition 2001; 4(2): 155-160.

Published online: 30 September 2001

DOI: https://doi.org/10.5223/kjpgn.2001.4.2.155

Helicobacter pylori vacA Mosaicism and New Primers for vacA Signal Sequence Indigenous to Korea

Yeon Hwa Ahn, Heung Ryel Kim, Ji Eun Lee1, Tae Sook Hwang2, Yon Ho Choe

Department of Pediatrics, Samsung Medical Center, Seoul, Korea.

1Department of Pediatrics, Inha University College of Medicine, Incheon, Korea.

2Department of Pathology, Inha University College of Medicine, Incheon, Korea.

cyh@smc.samsung.co.kr

Copyright © 2001 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition

Abstract

PURPOSE

Helicobacter pylori has been known to have diverse vacA allelic types. The purpose of the study was to identify vacA diversity in Korea and design new primers for signal sequence alleles indigenous to Korea.

METHODS

Fifty antral biopsy specimens, which had been proven to be H. pylori-positive, were examined for vacA status; signal sequence and mid-region. After PCR amplification and DNA sequencing, vacA alleles of Korean H. pylori strains were compared with those from other countries.

RESULTS

Among Korean H. pylori strains vacA alleles with all combinations of signal sequence and mid-region were found, with the exception of s1b or s2. vacA genotype s1c/m1 was predominant in Korea. We found that GGGAGCGTTR in s1a and GGGGYTATTG in s1c were the indigenous sequences to Korea and constructed the new Korean specific primers for the vacA signal sequence; VASK-F, VASK-R, S1AK-F, and S1CK-F.

CONCLUSION

This study showed that s1c/m1 is the predominant type of vacA allele in Korea. We designed new primers for the vacA signal sequence.

Keywords: Helicobacter pylori, vacA, Allele, Signal sequence, Mid-region

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