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Korean J Pediatr Gastroenterol Nutr. 2002 Mar;5(1):1-10. Korean.
Published online Mar 31, 2002.  https://doi.org/10.5223/kjpgn.2002.5.1.1
Copyright © 2002 The Korean Society of Pediatric Gastroenterology, Hepatology and Nutrition
Gastric Epithelial Cell Proliferation and Apoptosis in Children with Helicobacter pylori Infection
Ji Ah Jung, Jin Zhe,1 Woon Sup Han,2 and Jeong Wan Seo3
Department of Pediatrics, Pundang Jesaeng General Hospital, Sungnam, Korea.
1Department of Pathology, Cancer Hospital, Yanbian Jilin, China.
2Department of Pathology, Ewha Womans University College of Medicine, Seoul, Korea.
3Department of Pediatrics, Ewha Womans University College of Medicine, Seoul, Korea.
Abstract

PURPOSE

Dysregulation of gastric epithelial cell proliferation and apoptosis are important in development of ulcer, atrophy and neoplasia in Helicobacter pylori (H. pylori) infection. The aim of this study was to investigate the effect of infection of H. pylori on gastric epithelial cell proliferation and apoptosis in children.

METHODS

Histological grading by updated Sydney system, PCNA immunostaining and TUNEL method were performed in H. pylori positive (N=58) and negative (N=40) gastric biopsy specimens.

RESULTS

In H. pylori positive children, there were significantly higher grade of polymorphonuclear neutrophil activity (P=0.000), chronic inflammation (P=0.000), epithelial damage (P=0.000) and lymphoid follicles (P=0.000) than in H. pylori negative children. Intestinal metaplasia was not seen in H. pylori positive children. PCNA index was significantly different between H. pylori positive children (67.8±18.13) and H. pylori negative children (54.8±14.46, P=0.000). There was positive correlation between PCNA index and H. pylori density (r=0.277, P=0.007), polymorphonuclear neutrophil activity (r=0.280, P=0.007) and chronic inflammation (r=0.284, P=0.006). Apoptosis index of H. pylori positive children (0.44±0.447) was significantly higher than of H. pylori negative children (0.14±0.196, P=0.000). There was positive correlation between apoptosis index and H. pylori density (r=0.472, P=0.000), polymorphonuclear neutrophil activity (r=0.370, P=0.001) and chronic inflammation (r=0.483, P=0.000). There was positive correlation between PCNA index and apoptosis index (r=0.353, P=0.003).

CONCLUSION

The PCNA and apoptosis index in H. pylori positive children were significantly higher than in H. pylori negative children. This study suggested that gastric epithelial cell proliferation and apoptosis are important to pathogenesis of H. pylori infection in children.

Keywords: Helicobacter pylori; Gastric epithelial cell; Proliferation; Apoptosis; Chidren